In situ isolation of nuclei or nuclear proteins from adherent cells: a simple, effective method with less cytoplasmic contamination

BACKGROUND: Isolation of nuclei or nuclear proteins is a prerequisite for western blot, nuclear proteome profiling, and other evaluations of nuclear proteins. Here, we developed a simple method for in situ isolation of nuclei or nuclear proteins by in situ removing the extranuclear part of adherent...

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Detalles Bibliográficos
Autores principales: Qin, Ying, Zhou, Yun, Wang, Kun, Gu, Jiaxuan, Xiong, Zhihao, Zhang, Wendiao, Chen, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10120145/
https://www.ncbi.nlm.nih.gov/pubmed/37081568
http://dx.doi.org/10.1186/s40659-023-00429-2

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