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mRNA produced by VSW-3 RNAP has high-level translation efficiency with low inflammatory stimulation

In vitro preparation of mRNA is a key step for mRNA therapeutics. The widely used T7 RNA polymerase (RNAP) was shown to have many by-products during in vitro transcription (IVT) process, among which double-stranded RNA (dsRNA) is the major by-product to activate the intracellular immune response. He...

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Detalles Bibliográficos
Autores principales:	Wang, Guoquan, Cheng, Rui, Chen, Qiubing, Xu, Yuandong, Yu, Bingbing, Zhu, Bin, Yin, Hao, Xia, Heng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10120321/ https://www.ncbi.nlm.nih.gov/pubmed/37193555 http://dx.doi.org/10.1016/j.cellin.2022.100056

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