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Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system

Despite the advances in in vitro embryo production (IVP) over the years, the technique still has limitations that need to be overcome. In cell cultures, it is already well established that three-dimensional culture techniques are more physiological and similar to the in vivo development. Liquid marb...

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Autores principales: Ferronato, Giuliana de Avila, dos Santos, Carolina Mônica, Rosa, Paola Maria da S., Bridi, Alessandra, Perecin, Felipe, Meirelles, Flávio Vieira, Sangalli, Juliano Rodrigues, da Silveira, Juliano Coelho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10121032/
https://www.ncbi.nlm.nih.gov/pubmed/37083878
http://dx.doi.org/10.1371/journal.pone.0284809
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author Ferronato, Giuliana de Avila
dos Santos, Carolina Mônica
Rosa, Paola Maria da S.
Bridi, Alessandra
Perecin, Felipe
Meirelles, Flávio Vieira
Sangalli, Juliano Rodrigues
da Silveira, Juliano Coelho
author_facet Ferronato, Giuliana de Avila
dos Santos, Carolina Mônica
Rosa, Paola Maria da S.
Bridi, Alessandra
Perecin, Felipe
Meirelles, Flávio Vieira
Sangalli, Juliano Rodrigues
da Silveira, Juliano Coelho
author_sort Ferronato, Giuliana de Avila
collection PubMed
description Despite the advances in in vitro embryo production (IVP) over the years, the technique still has limitations that need to be overcome. In cell cultures, it is already well established that three-dimensional culture techniques are more physiological and similar to the in vivo development. Liquid marble (LM) is a three-dimensional system based on the use of a hydrophobic substance to create in vitro microbioreactors. Thus, we hypothesized that the LM system improves bovine in vitro oocyte maturation and embryo culture. In experiment I, bovine cumulus-oocyte complexes (COCs) were placed for in vitro maturation for 22h in two different groups: control (conventional 2D culture) and LM (three-dimensional culture). We found that oocyte nuclear maturation was not altered by the LM system, however it was observed a decrease in expression of genes important in the oocyte maturation process in cumulus cells of LM group (BCL2, EIF4E, and GAPDH). In experiment II, the COCs were conventionally matured and fertilized, and for culture, they were divided into LM or control groups. There was a decrease in blastocyst rate and cell counting, a down-regulation of miR-615 expression, and an increase in the DNA global methylation and hydroxymethylation in embryos of LM group. Therefore, for the bovine in vitro embryo production, this specific three-dimensional system did not present the advantages that we expected, but demonstrated that the embryos changed their development and epigenetics according to the culture system.
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spelling pubmed-101210322023-04-22 Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system Ferronato, Giuliana de Avila dos Santos, Carolina Mônica Rosa, Paola Maria da S. Bridi, Alessandra Perecin, Felipe Meirelles, Flávio Vieira Sangalli, Juliano Rodrigues da Silveira, Juliano Coelho PLoS One Research Article Despite the advances in in vitro embryo production (IVP) over the years, the technique still has limitations that need to be overcome. In cell cultures, it is already well established that three-dimensional culture techniques are more physiological and similar to the in vivo development. Liquid marble (LM) is a three-dimensional system based on the use of a hydrophobic substance to create in vitro microbioreactors. Thus, we hypothesized that the LM system improves bovine in vitro oocyte maturation and embryo culture. In experiment I, bovine cumulus-oocyte complexes (COCs) were placed for in vitro maturation for 22h in two different groups: control (conventional 2D culture) and LM (three-dimensional culture). We found that oocyte nuclear maturation was not altered by the LM system, however it was observed a decrease in expression of genes important in the oocyte maturation process in cumulus cells of LM group (BCL2, EIF4E, and GAPDH). In experiment II, the COCs were conventionally matured and fertilized, and for culture, they were divided into LM or control groups. There was a decrease in blastocyst rate and cell counting, a down-regulation of miR-615 expression, and an increase in the DNA global methylation and hydroxymethylation in embryos of LM group. Therefore, for the bovine in vitro embryo production, this specific three-dimensional system did not present the advantages that we expected, but demonstrated that the embryos changed their development and epigenetics according to the culture system. Public Library of Science 2023-04-21 /pmc/articles/PMC10121032/ /pubmed/37083878 http://dx.doi.org/10.1371/journal.pone.0284809 Text en © 2023 Ferronato et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ferronato, Giuliana de Avila
dos Santos, Carolina Mônica
Rosa, Paola Maria da S.
Bridi, Alessandra
Perecin, Felipe
Meirelles, Flávio Vieira
Sangalli, Juliano Rodrigues
da Silveira, Juliano Coelho
Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system
title Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system
title_full Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system
title_fullStr Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system
title_full_unstemmed Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system
title_short Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system
title_sort bovine in vitro oocyte maturation and embryo culture in liquid marbles 3d culture system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10121032/
https://www.ncbi.nlm.nih.gov/pubmed/37083878
http://dx.doi.org/10.1371/journal.pone.0284809
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