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Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system
Despite the advances in in vitro embryo production (IVP) over the years, the technique still has limitations that need to be overcome. In cell cultures, it is already well established that three-dimensional culture techniques are more physiological and similar to the in vivo development. Liquid marb...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10121032/ https://www.ncbi.nlm.nih.gov/pubmed/37083878 http://dx.doi.org/10.1371/journal.pone.0284809 |
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author | Ferronato, Giuliana de Avila dos Santos, Carolina Mônica Rosa, Paola Maria da S. Bridi, Alessandra Perecin, Felipe Meirelles, Flávio Vieira Sangalli, Juliano Rodrigues da Silveira, Juliano Coelho |
author_facet | Ferronato, Giuliana de Avila dos Santos, Carolina Mônica Rosa, Paola Maria da S. Bridi, Alessandra Perecin, Felipe Meirelles, Flávio Vieira Sangalli, Juliano Rodrigues da Silveira, Juliano Coelho |
author_sort | Ferronato, Giuliana de Avila |
collection | PubMed |
description | Despite the advances in in vitro embryo production (IVP) over the years, the technique still has limitations that need to be overcome. In cell cultures, it is already well established that three-dimensional culture techniques are more physiological and similar to the in vivo development. Liquid marble (LM) is a three-dimensional system based on the use of a hydrophobic substance to create in vitro microbioreactors. Thus, we hypothesized that the LM system improves bovine in vitro oocyte maturation and embryo culture. In experiment I, bovine cumulus-oocyte complexes (COCs) were placed for in vitro maturation for 22h in two different groups: control (conventional 2D culture) and LM (three-dimensional culture). We found that oocyte nuclear maturation was not altered by the LM system, however it was observed a decrease in expression of genes important in the oocyte maturation process in cumulus cells of LM group (BCL2, EIF4E, and GAPDH). In experiment II, the COCs were conventionally matured and fertilized, and for culture, they were divided into LM or control groups. There was a decrease in blastocyst rate and cell counting, a down-regulation of miR-615 expression, and an increase in the DNA global methylation and hydroxymethylation in embryos of LM group. Therefore, for the bovine in vitro embryo production, this specific three-dimensional system did not present the advantages that we expected, but demonstrated that the embryos changed their development and epigenetics according to the culture system. |
format | Online Article Text |
id | pubmed-10121032 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-101210322023-04-22 Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system Ferronato, Giuliana de Avila dos Santos, Carolina Mônica Rosa, Paola Maria da S. Bridi, Alessandra Perecin, Felipe Meirelles, Flávio Vieira Sangalli, Juliano Rodrigues da Silveira, Juliano Coelho PLoS One Research Article Despite the advances in in vitro embryo production (IVP) over the years, the technique still has limitations that need to be overcome. In cell cultures, it is already well established that three-dimensional culture techniques are more physiological and similar to the in vivo development. Liquid marble (LM) is a three-dimensional system based on the use of a hydrophobic substance to create in vitro microbioreactors. Thus, we hypothesized that the LM system improves bovine in vitro oocyte maturation and embryo culture. In experiment I, bovine cumulus-oocyte complexes (COCs) were placed for in vitro maturation for 22h in two different groups: control (conventional 2D culture) and LM (three-dimensional culture). We found that oocyte nuclear maturation was not altered by the LM system, however it was observed a decrease in expression of genes important in the oocyte maturation process in cumulus cells of LM group (BCL2, EIF4E, and GAPDH). In experiment II, the COCs were conventionally matured and fertilized, and for culture, they were divided into LM or control groups. There was a decrease in blastocyst rate and cell counting, a down-regulation of miR-615 expression, and an increase in the DNA global methylation and hydroxymethylation in embryos of LM group. Therefore, for the bovine in vitro embryo production, this specific three-dimensional system did not present the advantages that we expected, but demonstrated that the embryos changed their development and epigenetics according to the culture system. Public Library of Science 2023-04-21 /pmc/articles/PMC10121032/ /pubmed/37083878 http://dx.doi.org/10.1371/journal.pone.0284809 Text en © 2023 Ferronato et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ferronato, Giuliana de Avila dos Santos, Carolina Mônica Rosa, Paola Maria da S. Bridi, Alessandra Perecin, Felipe Meirelles, Flávio Vieira Sangalli, Juliano Rodrigues da Silveira, Juliano Coelho Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system |
title | Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system |
title_full | Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system |
title_fullStr | Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system |
title_full_unstemmed | Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system |
title_short | Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system |
title_sort | bovine in vitro oocyte maturation and embryo culture in liquid marbles 3d culture system |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10121032/ https://www.ncbi.nlm.nih.gov/pubmed/37083878 http://dx.doi.org/10.1371/journal.pone.0284809 |
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