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Transcriptomics unravels molecular changes associated with cilia and COVID-19 in chronic rhinosinusitis with nasal polyps

Chronic rhinosinusitis with nasal polyps (CRSwNP) is a common upper respiratory tract complication where the pathogenesis is largely unknown. Herein, we investigated the transcriptome profile in nasal mucosa biopsies of CRSwNP patients and healthy individuals. We further integrated the transcriptomi...

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Autores principales: Torinsson Naluai, Åsa, Östensson, Malin, Fowler, Philippa C., Abrahamsson, Sanna, Andersson, Björn, Lassesson, Stina, Jacobsson, Frida, Oscarsson, Martin, Bohman, Anton, Harandi, Ali M., Bende, Mats
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10121071/
https://www.ncbi.nlm.nih.gov/pubmed/37085563
http://dx.doi.org/10.1038/s41598-023-32944-3
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author Torinsson Naluai, Åsa
Östensson, Malin
Fowler, Philippa C.
Abrahamsson, Sanna
Andersson, Björn
Lassesson, Stina
Jacobsson, Frida
Oscarsson, Martin
Bohman, Anton
Harandi, Ali M.
Bende, Mats
author_facet Torinsson Naluai, Åsa
Östensson, Malin
Fowler, Philippa C.
Abrahamsson, Sanna
Andersson, Björn
Lassesson, Stina
Jacobsson, Frida
Oscarsson, Martin
Bohman, Anton
Harandi, Ali M.
Bende, Mats
author_sort Torinsson Naluai, Åsa
collection PubMed
description Chronic rhinosinusitis with nasal polyps (CRSwNP) is a common upper respiratory tract complication where the pathogenesis is largely unknown. Herein, we investigated the transcriptome profile in nasal mucosa biopsies of CRSwNP patients and healthy individuals. We further integrated the transcriptomics data with genes located in chromosomal regions containing genome-wide significant gene variants for COVID-19. Among the most significantly upregulated genes in polyp mucosa were CCL18, CLEC4G, CCL13 and SLC9A3. Pathways involving “Ciliated epithelial cells” were the most differentially expressed molecular pathways when polyp mucosa and non-polyp mucosa from the same patient was compared. Natural killer T-cell (NKT) and viral pathways were the most statistically significant pathways in the mucosa of CRSwNP patients compared with those of healthy control individuals. Upregulated genes in polyp mucosa, located within the genome-wide associated regions of COVID-19, included LZTFL1, CCR9, SLC6A20, IFNAR1, IFNAR2 and IL10RB. Interestingly, the second most over-expressed gene in our study, CLEC4G, has been shown to bind directly to SARS-CoV-2 spike's N-terminal domain and mediate its entry and infection. Our results on altered expression of genes related to cilia and viruses point to the de-regulation of viral defenses in CRSwNP patients, and may give clues to future intervention strategies.
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spelling pubmed-101210712023-04-23 Transcriptomics unravels molecular changes associated with cilia and COVID-19 in chronic rhinosinusitis with nasal polyps Torinsson Naluai, Åsa Östensson, Malin Fowler, Philippa C. Abrahamsson, Sanna Andersson, Björn Lassesson, Stina Jacobsson, Frida Oscarsson, Martin Bohman, Anton Harandi, Ali M. Bende, Mats Sci Rep Article Chronic rhinosinusitis with nasal polyps (CRSwNP) is a common upper respiratory tract complication where the pathogenesis is largely unknown. Herein, we investigated the transcriptome profile in nasal mucosa biopsies of CRSwNP patients and healthy individuals. We further integrated the transcriptomics data with genes located in chromosomal regions containing genome-wide significant gene variants for COVID-19. Among the most significantly upregulated genes in polyp mucosa were CCL18, CLEC4G, CCL13 and SLC9A3. Pathways involving “Ciliated epithelial cells” were the most differentially expressed molecular pathways when polyp mucosa and non-polyp mucosa from the same patient was compared. Natural killer T-cell (NKT) and viral pathways were the most statistically significant pathways in the mucosa of CRSwNP patients compared with those of healthy control individuals. Upregulated genes in polyp mucosa, located within the genome-wide associated regions of COVID-19, included LZTFL1, CCR9, SLC6A20, IFNAR1, IFNAR2 and IL10RB. Interestingly, the second most over-expressed gene in our study, CLEC4G, has been shown to bind directly to SARS-CoV-2 spike's N-terminal domain and mediate its entry and infection. Our results on altered expression of genes related to cilia and viruses point to the de-regulation of viral defenses in CRSwNP patients, and may give clues to future intervention strategies. Nature Publishing Group UK 2023-04-21 /pmc/articles/PMC10121071/ /pubmed/37085563 http://dx.doi.org/10.1038/s41598-023-32944-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Torinsson Naluai, Åsa
Östensson, Malin
Fowler, Philippa C.
Abrahamsson, Sanna
Andersson, Björn
Lassesson, Stina
Jacobsson, Frida
Oscarsson, Martin
Bohman, Anton
Harandi, Ali M.
Bende, Mats
Transcriptomics unravels molecular changes associated with cilia and COVID-19 in chronic rhinosinusitis with nasal polyps
title Transcriptomics unravels molecular changes associated with cilia and COVID-19 in chronic rhinosinusitis with nasal polyps
title_full Transcriptomics unravels molecular changes associated with cilia and COVID-19 in chronic rhinosinusitis with nasal polyps
title_fullStr Transcriptomics unravels molecular changes associated with cilia and COVID-19 in chronic rhinosinusitis with nasal polyps
title_full_unstemmed Transcriptomics unravels molecular changes associated with cilia and COVID-19 in chronic rhinosinusitis with nasal polyps
title_short Transcriptomics unravels molecular changes associated with cilia and COVID-19 in chronic rhinosinusitis with nasal polyps
title_sort transcriptomics unravels molecular changes associated with cilia and covid-19 in chronic rhinosinusitis with nasal polyps
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10121071/
https://www.ncbi.nlm.nih.gov/pubmed/37085563
http://dx.doi.org/10.1038/s41598-023-32944-3
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