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Miniscope-LFOV: A large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals

Imaging large-population, single-cell fluorescent dynamics in freely behaving animals larger than mice remains a key endeavor of neuroscience. We present a large-field-of-view open-source miniature microscope (MiniLFOV) designed for large-scale (3.6 mm × 2.7 mm), cellular resolution neural imaging i...

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Autores principales: Guo, Changliang, Blair, Garrett J., Sehgal, Megha, Sangiuliano Jimka, Federico N., Bellafard, Arash, Silva, Alcino J., Golshani, Peyman, Basso, Michele A., Blair, Hugh Tad, Aharoni, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10121160/
https://www.ncbi.nlm.nih.gov/pubmed/37083539
http://dx.doi.org/10.1126/sciadv.adg3918
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author Guo, Changliang
Blair, Garrett J.
Sehgal, Megha
Sangiuliano Jimka, Federico N.
Bellafard, Arash
Silva, Alcino J.
Golshani, Peyman
Basso, Michele A.
Blair, Hugh Tad
Aharoni, Daniel
author_facet Guo, Changliang
Blair, Garrett J.
Sehgal, Megha
Sangiuliano Jimka, Federico N.
Bellafard, Arash
Silva, Alcino J.
Golshani, Peyman
Basso, Michele A.
Blair, Hugh Tad
Aharoni, Daniel
author_sort Guo, Changliang
collection PubMed
description Imaging large-population, single-cell fluorescent dynamics in freely behaving animals larger than mice remains a key endeavor of neuroscience. We present a large-field-of-view open-source miniature microscope (MiniLFOV) designed for large-scale (3.6 mm × 2.7 mm), cellular resolution neural imaging in freely behaving rats. It has an electrically adjustable working distance of up to 3.5 mm ± 100 μm, incorporates an absolute head orientation sensor, and weighs only 13.9 g. The MiniLFOV is capable of both deep brain and cortical imaging and has been validated in freely behaving rats by simultaneously imaging >1000 GCaMP7s-expressing neurons in the hippocampal CA1 layer and in head-fixed mice by simultaneously imaging ~2000 neurons in the dorsal cortex through a cranial window. The MiniLFOV also supports optional wire-free operation using a novel, wire-free data acquisition expansion board. We expect that this new open-source implementation of the UCLA Miniscope platform will enable researchers to address novel hypotheses concerning brain function in freely behaving animals.
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spelling pubmed-101211602023-04-22 Miniscope-LFOV: A large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals Guo, Changliang Blair, Garrett J. Sehgal, Megha Sangiuliano Jimka, Federico N. Bellafard, Arash Silva, Alcino J. Golshani, Peyman Basso, Michele A. Blair, Hugh Tad Aharoni, Daniel Sci Adv Physical and Materials Sciences Imaging large-population, single-cell fluorescent dynamics in freely behaving animals larger than mice remains a key endeavor of neuroscience. We present a large-field-of-view open-source miniature microscope (MiniLFOV) designed for large-scale (3.6 mm × 2.7 mm), cellular resolution neural imaging in freely behaving rats. It has an electrically adjustable working distance of up to 3.5 mm ± 100 μm, incorporates an absolute head orientation sensor, and weighs only 13.9 g. The MiniLFOV is capable of both deep brain and cortical imaging and has been validated in freely behaving rats by simultaneously imaging >1000 GCaMP7s-expressing neurons in the hippocampal CA1 layer and in head-fixed mice by simultaneously imaging ~2000 neurons in the dorsal cortex through a cranial window. The MiniLFOV also supports optional wire-free operation using a novel, wire-free data acquisition expansion board. We expect that this new open-source implementation of the UCLA Miniscope platform will enable researchers to address novel hypotheses concerning brain function in freely behaving animals. American Association for the Advancement of Science 2023-04-21 /pmc/articles/PMC10121160/ /pubmed/37083539 http://dx.doi.org/10.1126/sciadv.adg3918 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Physical and Materials Sciences
Guo, Changliang
Blair, Garrett J.
Sehgal, Megha
Sangiuliano Jimka, Federico N.
Bellafard, Arash
Silva, Alcino J.
Golshani, Peyman
Basso, Michele A.
Blair, Hugh Tad
Aharoni, Daniel
Miniscope-LFOV: A large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals
title Miniscope-LFOV: A large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals
title_full Miniscope-LFOV: A large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals
title_fullStr Miniscope-LFOV: A large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals
title_full_unstemmed Miniscope-LFOV: A large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals
title_short Miniscope-LFOV: A large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals
title_sort miniscope-lfov: a large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals
topic Physical and Materials Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10121160/
https://www.ncbi.nlm.nih.gov/pubmed/37083539
http://dx.doi.org/10.1126/sciadv.adg3918
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