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Senescent epithelial cells remodel the microenvironment for the progression of oral submucous fibrosis through secreting TGF-β1
OBJECTIVES: Cellular senescence is strongly associated with fibrosis and tumorigenesis. However, whether the epithelium of oral submucous fibrosis (OSF) undergoes premature senescence remains unclear. This study investigates the roles of senescent epithelial cells in OSF. METHODS: The immunohistoche...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10122456/ https://www.ncbi.nlm.nih.gov/pubmed/37096061 http://dx.doi.org/10.7717/peerj.15158 |
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author | Wang, Zijia Han, Ying Peng, Ying Shao, Shuhui Nie, Huanquan Xia, Kun Xiong, Haofeng Su, Tong |
author_facet | Wang, Zijia Han, Ying Peng, Ying Shao, Shuhui Nie, Huanquan Xia, Kun Xiong, Haofeng Su, Tong |
author_sort | Wang, Zijia |
collection | PubMed |
description | OBJECTIVES: Cellular senescence is strongly associated with fibrosis and tumorigenesis. However, whether the epithelium of oral submucous fibrosis (OSF) undergoes premature senescence remains unclear. This study investigates the roles of senescent epithelial cells in OSF. METHODS: The immunohistochemistry and Sudan black B staining were performed to identify epithelium senescence in OSF tissues. Arecoline was used to induce human oral keratinocytes (HOKs) senescence. The cell morphology, senescence-associated β galactosidase activity, cell counting Kit 8, immunofluorescence, quantitative real-time PCR, and western blot assay were used to identification of senescent HOKs. The enzyme-linked immunosorbent assay was exerted to evaluate the levels of transforming growth factor β1 (TGF-β1) in the supernatants of HOKs treated with or without arecoline. RESULTS: The senescence-associated markers, p16 and p21, were overexpressed in OSF epithelium. These expressions were correlated with alpha-smooth actin (α-SMA) positively and proliferating cell nuclear antigen (PCNA) negatively. Moreover, Sudan black staining showed that there was more lipofuscin in OSF epithelium. In vitro, HOKs treated with arecoline showed senescence-associated characteristics including enlarged and flattened morphology, senescence-associated β galactosidase staining, cell growth arrest, γH2A.X foci, upregulation of p53, p21, and TGF-β1 protein levels. Moreover, senescent HOKs secreted more TGF-β1. CONCLUSIONS: Senescent epithelial cells are involved in OSF progression and may become a promising target for OSF treatment. |
format | Online Article Text |
id | pubmed-10122456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101224562023-04-23 Senescent epithelial cells remodel the microenvironment for the progression of oral submucous fibrosis through secreting TGF-β1 Wang, Zijia Han, Ying Peng, Ying Shao, Shuhui Nie, Huanquan Xia, Kun Xiong, Haofeng Su, Tong PeerJ Biochemistry OBJECTIVES: Cellular senescence is strongly associated with fibrosis and tumorigenesis. However, whether the epithelium of oral submucous fibrosis (OSF) undergoes premature senescence remains unclear. This study investigates the roles of senescent epithelial cells in OSF. METHODS: The immunohistochemistry and Sudan black B staining were performed to identify epithelium senescence in OSF tissues. Arecoline was used to induce human oral keratinocytes (HOKs) senescence. The cell morphology, senescence-associated β galactosidase activity, cell counting Kit 8, immunofluorescence, quantitative real-time PCR, and western blot assay were used to identification of senescent HOKs. The enzyme-linked immunosorbent assay was exerted to evaluate the levels of transforming growth factor β1 (TGF-β1) in the supernatants of HOKs treated with or without arecoline. RESULTS: The senescence-associated markers, p16 and p21, were overexpressed in OSF epithelium. These expressions were correlated with alpha-smooth actin (α-SMA) positively and proliferating cell nuclear antigen (PCNA) negatively. Moreover, Sudan black staining showed that there was more lipofuscin in OSF epithelium. In vitro, HOKs treated with arecoline showed senescence-associated characteristics including enlarged and flattened morphology, senescence-associated β galactosidase staining, cell growth arrest, γH2A.X foci, upregulation of p53, p21, and TGF-β1 protein levels. Moreover, senescent HOKs secreted more TGF-β1. CONCLUSIONS: Senescent epithelial cells are involved in OSF progression and may become a promising target for OSF treatment. PeerJ Inc. 2023-04-19 /pmc/articles/PMC10122456/ /pubmed/37096061 http://dx.doi.org/10.7717/peerj.15158 Text en ©2023 Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Biochemistry Wang, Zijia Han, Ying Peng, Ying Shao, Shuhui Nie, Huanquan Xia, Kun Xiong, Haofeng Su, Tong Senescent epithelial cells remodel the microenvironment for the progression of oral submucous fibrosis through secreting TGF-β1 |
title | Senescent epithelial cells remodel the microenvironment for the progression of oral submucous fibrosis through secreting TGF-β1 |
title_full | Senescent epithelial cells remodel the microenvironment for the progression of oral submucous fibrosis through secreting TGF-β1 |
title_fullStr | Senescent epithelial cells remodel the microenvironment for the progression of oral submucous fibrosis through secreting TGF-β1 |
title_full_unstemmed | Senescent epithelial cells remodel the microenvironment for the progression of oral submucous fibrosis through secreting TGF-β1 |
title_short | Senescent epithelial cells remodel the microenvironment for the progression of oral submucous fibrosis through secreting TGF-β1 |
title_sort | senescent epithelial cells remodel the microenvironment for the progression of oral submucous fibrosis through secreting tgf-β1 |
topic | Biochemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10122456/ https://www.ncbi.nlm.nih.gov/pubmed/37096061 http://dx.doi.org/10.7717/peerj.15158 |
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