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Computational design and molecular dynamics simulations suggest the mode of substrate binding in ceramide synthases

Until now, membrane-protein stabilization has relied on iterations of mutations and screening. We now validate a one-step algorithm, mPROSS, for stabilizing membrane proteins directly from an AlphaFold2 model structure. Applied to the lipid-generating enzyme, ceramide synthase, 37 designed mutations...

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Detalles Bibliográficos
Autores principales:	Zelnik, Iris D., Mestre, Beatriz, Weinstein, Jonathan J., Dingjan, Tamir, Izrailov, Stav, Ben-Dor, Shifra, Fleishman, Sarel J., Futerman, Anthony H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10122649/ https://www.ncbi.nlm.nih.gov/pubmed/37087500 http://dx.doi.org/10.1038/s41467-023-38047-x

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