Validation of portable electronic equipment (Accutrend(®) Plus) to determine glucose, total cholesterol, and triglycerides in rats (Rattus) and dogs (Canis lupus familiaris)

OBJECTIVE: This study aimed to validate the analytical precision of the Accutrend(®) Plus portable electronic equipment (PE) to determine glucose (GLU), total cholesterol (TC), and triglycerides (TG) in rats and dogs using the conventional laboratory method (CM) as a reference. MATERIALS AND METHODS: To determine the analytical accuracy of the Accutrend(®) Plus in the measurement of GLU, CT, and TG. The EP-9-A2 guide (Clinical and Laboratory Standards Institute), Bland-Altman graphical analysis, and Lin’s correlation coefficient of concordance (CCC) were implemented. RESULTS: The average differences (p > 0.05) between PE and CM for GLU, TC, and TG were 2.21, 1.20, and 0.72 mg·dl(−1), respectively, in rats and 1.06, 4.30, and 2.41 mg·dl(−1), respectively, in dogs (p > 0.05). Both methods showed a linear relationship with Pearson’s correlation coefficients > 0.96 and R(2) > 0.97 for the three biochemical indicators evaluated in both species. The GLU, TC, and TG values obtained by the PE were substantial, as evident from Lin’s CCC > 0.96. CONCLUSION: The PE Accutrend(®) Plus is potent for monitoring GLU, TC, and TG in rats and dogs because of its precision and ability to facilitate measurement by reducing stress in animals during sampling.