Cas9 off-target binding to the promoter of bacterial genes leads to silencing and toxicity

Genetic tools derived from the Cas9 RNA-guided nuclease are providing essential capabilities to study and engineer bacteria. While the importance of off-target effects was noted early in Cas9’s application to mammalian cells, off-target cleavage by Cas9 in bacterial genomes is easily avoided due to...

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Detalles Bibliográficos
Autores principales: Rostain, William, Grebert, Theophile, Vyhovskyi, Danylo, Pizarro, Paula Thiel, Tshinsele-Van Bellingen, Gatwa, Cui, Lun, Bikard, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123097/
https://www.ncbi.nlm.nih.gov/pubmed/36929199
http://dx.doi.org/10.1093/nar/gkad170

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123097/
https://www.ncbi.nlm.nih.gov/pubmed/36929199
http://dx.doi.org/10.1093/nar/gkad170

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