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Cas9 off-target binding to the promoter of bacterial genes leads to silencing and toxicity
Genetic tools derived from the Cas9 RNA-guided nuclease are providing essential capabilities to study and engineer bacteria. While the importance of off-target effects was noted early in Cas9’s application to mammalian cells, off-target cleavage by Cas9 in bacterial genomes is easily avoided due to...
Autores principales: | Rostain, William, Grebert, Theophile, Vyhovskyi, Danylo, Pizarro, Paula Thiel, Tshinsele-Van Bellingen, Gatwa, Cui, Lun, Bikard, David |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123097/ https://www.ncbi.nlm.nih.gov/pubmed/36929199 http://dx.doi.org/10.1093/nar/gkad170 |
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