Single-molecule analysis of DNA-binding proteins from nuclear extracts (SMADNE)

Single-molecule characterization of protein–DNA dynamics provides unprecedented mechanistic details about numerous nuclear processes. Here, we describe a new method that rapidly generates single-molecule information with fluorescently tagged proteins isolated from nuclear extracts of human cells. We...

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Detalles Bibliográficos
Autores principales: Schaich, Matthew A, Schnable, Brittani L, Kumar, Namrata, Roginskaya, Vera, Jakielski, Rachel C, Urban, Roman, Zhong, Zhou, Kad, Neil M, Van Houten, Bennett
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123111/
https://www.ncbi.nlm.nih.gov/pubmed/36861323
http://dx.doi.org/10.1093/nar/gkad095

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