Cargando…

A small-diameter vascular graft immobilized peptides for capturing endothelial colony-forming cells

Combining synthetic polymers and biomacromolecules prevents the occurrence of thrombogenicity and intimal hyperplasia in small-diameter vascular grafts (SDVGs). In the present study, an electrospinning poly (L)-lactic acid (PLLA) bilayered scaffold is developed to prevent thrombosis after implantati...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Yaqi, Yin, Lu, Gao, Shuai, Long, Xiaojing, Du, Zhanhui, Zhou, Yingchao, Zhao, Shuiyan, Cao, Yue, Pan, Silin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123284/
https://www.ncbi.nlm.nih.gov/pubmed/37101749
http://dx.doi.org/10.3389/fbioe.2023.1154986
_version_ 1785029641000452096
author Tang, Yaqi
Yin, Lu
Gao, Shuai
Long, Xiaojing
Du, Zhanhui
Zhou, Yingchao
Zhao, Shuiyan
Cao, Yue
Pan, Silin
author_facet Tang, Yaqi
Yin, Lu
Gao, Shuai
Long, Xiaojing
Du, Zhanhui
Zhou, Yingchao
Zhao, Shuiyan
Cao, Yue
Pan, Silin
author_sort Tang, Yaqi
collection PubMed
description Combining synthetic polymers and biomacromolecules prevents the occurrence of thrombogenicity and intimal hyperplasia in small-diameter vascular grafts (SDVGs). In the present study, an electrospinning poly (L)-lactic acid (PLLA) bilayered scaffold is developed to prevent thrombosis after implantation by promoting the capture and differentiation of endothelial colony-forming cells (ECFCs). The scaffold consists of an outer PLLA scaffold and an inner porous PLLA biomimetic membrane combined with heparin (Hep), peptide Gly-Gly-Gly-Arg-Glu-Asp-Val (GGG-REDV), and vascular endothelial growth factor (VEGF). Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and contact angle goniometry were performed to determine successful synthesis. The tensile strength of the outer layer was obtained using the recorded stress/strain curves, and hemocompatibility was evaluated using the blood clotting test. The proliferation, function, and differentiation properties of ECFCs were measured on various surfaces. Scanning electronic microscopy (SEM) was used to observe the morphology of ECFCs on the surface. The outer layer of scaffolds exhibited a similar strain and stress performance as the human saphenous vein via the tensile experiment. The contact angle decreased continuously until it reached 56° after REDV/VEGF modification, and SEM images of platelet adhesion showed a better hemocompatibility surface after modification. The ECFCs were captured using the REDV + VEGF + surface successfully under flow conditions. The expression of mature ECs was constantly increased with the culture of ECFCs on REDV + VEGF + surfaces. SEM images showed that the ECFCs captured by the REDV + VEGF + surface formed capillary-like structures after 4 weeks of culture. The SDVGs modified by REDV combined with VEGF promoted ECFC capture and rapid differentiation into ECs, forming capillary-like structures in vitro. The bilayered SDVGs could be used as vascular devices that achieved a high patency rate and rapid re-endothelialization.
format Online
Article
Text
id pubmed-10123284
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-101232842023-04-25 A small-diameter vascular graft immobilized peptides for capturing endothelial colony-forming cells Tang, Yaqi Yin, Lu Gao, Shuai Long, Xiaojing Du, Zhanhui Zhou, Yingchao Zhao, Shuiyan Cao, Yue Pan, Silin Front Bioeng Biotechnol Bioengineering and Biotechnology Combining synthetic polymers and biomacromolecules prevents the occurrence of thrombogenicity and intimal hyperplasia in small-diameter vascular grafts (SDVGs). In the present study, an electrospinning poly (L)-lactic acid (PLLA) bilayered scaffold is developed to prevent thrombosis after implantation by promoting the capture and differentiation of endothelial colony-forming cells (ECFCs). The scaffold consists of an outer PLLA scaffold and an inner porous PLLA biomimetic membrane combined with heparin (Hep), peptide Gly-Gly-Gly-Arg-Glu-Asp-Val (GGG-REDV), and vascular endothelial growth factor (VEGF). Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and contact angle goniometry were performed to determine successful synthesis. The tensile strength of the outer layer was obtained using the recorded stress/strain curves, and hemocompatibility was evaluated using the blood clotting test. The proliferation, function, and differentiation properties of ECFCs were measured on various surfaces. Scanning electronic microscopy (SEM) was used to observe the morphology of ECFCs on the surface. The outer layer of scaffolds exhibited a similar strain and stress performance as the human saphenous vein via the tensile experiment. The contact angle decreased continuously until it reached 56° after REDV/VEGF modification, and SEM images of platelet adhesion showed a better hemocompatibility surface after modification. The ECFCs were captured using the REDV + VEGF + surface successfully under flow conditions. The expression of mature ECs was constantly increased with the culture of ECFCs on REDV + VEGF + surfaces. SEM images showed that the ECFCs captured by the REDV + VEGF + surface formed capillary-like structures after 4 weeks of culture. The SDVGs modified by REDV combined with VEGF promoted ECFC capture and rapid differentiation into ECs, forming capillary-like structures in vitro. The bilayered SDVGs could be used as vascular devices that achieved a high patency rate and rapid re-endothelialization. Frontiers Media S.A. 2023-04-10 /pmc/articles/PMC10123284/ /pubmed/37101749 http://dx.doi.org/10.3389/fbioe.2023.1154986 Text en Copyright © 2023 Tang, Yin, Gao, Long, Du, Zhou, Zhao, Cao and Pan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Tang, Yaqi
Yin, Lu
Gao, Shuai
Long, Xiaojing
Du, Zhanhui
Zhou, Yingchao
Zhao, Shuiyan
Cao, Yue
Pan, Silin
A small-diameter vascular graft immobilized peptides for capturing endothelial colony-forming cells
title A small-diameter vascular graft immobilized peptides for capturing endothelial colony-forming cells
title_full A small-diameter vascular graft immobilized peptides for capturing endothelial colony-forming cells
title_fullStr A small-diameter vascular graft immobilized peptides for capturing endothelial colony-forming cells
title_full_unstemmed A small-diameter vascular graft immobilized peptides for capturing endothelial colony-forming cells
title_short A small-diameter vascular graft immobilized peptides for capturing endothelial colony-forming cells
title_sort small-diameter vascular graft immobilized peptides for capturing endothelial colony-forming cells
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123284/
https://www.ncbi.nlm.nih.gov/pubmed/37101749
http://dx.doi.org/10.3389/fbioe.2023.1154986
work_keys_str_mv AT tangyaqi asmalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT yinlu asmalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT gaoshuai asmalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT longxiaojing asmalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT duzhanhui asmalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT zhouyingchao asmalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT zhaoshuiyan asmalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT caoyue asmalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT pansilin asmalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT tangyaqi smalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT yinlu smalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT gaoshuai smalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT longxiaojing smalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT duzhanhui smalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT zhouyingchao smalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT zhaoshuiyan smalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT caoyue smalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells
AT pansilin smalldiametervasculargraftimmobilizedpeptidesforcapturingendothelialcolonyformingcells