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Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer

Cellular interactions within the bone marrow microenvironment modulate the properties of subsets of leukemic cells leading to the development of drug-resistant phenotypes. The intercellular transfer of proteins and organelles contributes to this process but the set of transferred proteins and their...

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Autores principales: Nevárez-Ramírez, Abraham Josué, Guzmán-Ortiz, Ana Laura, Cortes-Reynosa, Pedro, Perez-Salazar, Eduardo, Jaimes-Ortega, Gustavo Alberto, Valle-Rios, Ricardo, Marín-Hernández, Álvaro, Rodríguez-Zavala, José S., Ruiz-May, Eliel, Castrejón-Flores, José Luis, Quezada, Héctor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123657/
https://www.ncbi.nlm.nih.gov/pubmed/37092456
http://dx.doi.org/10.3390/proteomes11020015
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author Nevárez-Ramírez, Abraham Josué
Guzmán-Ortiz, Ana Laura
Cortes-Reynosa, Pedro
Perez-Salazar, Eduardo
Jaimes-Ortega, Gustavo Alberto
Valle-Rios, Ricardo
Marín-Hernández, Álvaro
Rodríguez-Zavala, José S.
Ruiz-May, Eliel
Castrejón-Flores, José Luis
Quezada, Héctor
author_facet Nevárez-Ramírez, Abraham Josué
Guzmán-Ortiz, Ana Laura
Cortes-Reynosa, Pedro
Perez-Salazar, Eduardo
Jaimes-Ortega, Gustavo Alberto
Valle-Rios, Ricardo
Marín-Hernández, Álvaro
Rodríguez-Zavala, José S.
Ruiz-May, Eliel
Castrejón-Flores, José Luis
Quezada, Héctor
author_sort Nevárez-Ramírez, Abraham Josué
collection PubMed
description Cellular interactions within the bone marrow microenvironment modulate the properties of subsets of leukemic cells leading to the development of drug-resistant phenotypes. The intercellular transfer of proteins and organelles contributes to this process but the set of transferred proteins and their effects in the receiving cells remain unclear. This study aimed to detect the intercellular protein transfer from mouse bone marrow stromal cells (OP9 cell line) to human T-lymphoblasts (CCRF-CEM cell line) using nanoLC-MS/MS-based shotgun proteomics in a 3D co-culture system. After 24 h of co-culture, 1513 and 67 proteins from human and mouse origin, respectively, were identified in CCRF-CEM cells. The presence of mouse proteins in the human cell line, detected by analyzing the differences in amino acid sequences of orthologous peptides, was interpreted as the result of intercellular transfer. The transferred proteins might have contributed to the observed resistance to vincristine, methotrexate, and hydrogen peroxide in the co-cultured leukemic cells. Our results suggest that shotgun proteomic analyses of co-cultured cells from different species could be a simple option to get a preliminary survey of the proteins exchanged among interacting cells.
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spelling pubmed-101236572023-04-25 Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer Nevárez-Ramírez, Abraham Josué Guzmán-Ortiz, Ana Laura Cortes-Reynosa, Pedro Perez-Salazar, Eduardo Jaimes-Ortega, Gustavo Alberto Valle-Rios, Ricardo Marín-Hernández, Álvaro Rodríguez-Zavala, José S. Ruiz-May, Eliel Castrejón-Flores, José Luis Quezada, Héctor Proteomes Article Cellular interactions within the bone marrow microenvironment modulate the properties of subsets of leukemic cells leading to the development of drug-resistant phenotypes. The intercellular transfer of proteins and organelles contributes to this process but the set of transferred proteins and their effects in the receiving cells remain unclear. This study aimed to detect the intercellular protein transfer from mouse bone marrow stromal cells (OP9 cell line) to human T-lymphoblasts (CCRF-CEM cell line) using nanoLC-MS/MS-based shotgun proteomics in a 3D co-culture system. After 24 h of co-culture, 1513 and 67 proteins from human and mouse origin, respectively, were identified in CCRF-CEM cells. The presence of mouse proteins in the human cell line, detected by analyzing the differences in amino acid sequences of orthologous peptides, was interpreted as the result of intercellular transfer. The transferred proteins might have contributed to the observed resistance to vincristine, methotrexate, and hydrogen peroxide in the co-cultured leukemic cells. Our results suggest that shotgun proteomic analyses of co-cultured cells from different species could be a simple option to get a preliminary survey of the proteins exchanged among interacting cells. MDPI 2023-04-05 /pmc/articles/PMC10123657/ /pubmed/37092456 http://dx.doi.org/10.3390/proteomes11020015 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nevárez-Ramírez, Abraham Josué
Guzmán-Ortiz, Ana Laura
Cortes-Reynosa, Pedro
Perez-Salazar, Eduardo
Jaimes-Ortega, Gustavo Alberto
Valle-Rios, Ricardo
Marín-Hernández, Álvaro
Rodríguez-Zavala, José S.
Ruiz-May, Eliel
Castrejón-Flores, José Luis
Quezada, Héctor
Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer
title Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer
title_full Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer
title_fullStr Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer
title_full_unstemmed Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer
title_short Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer
title_sort shotgun proteomics of co-cultured leukemic and bone marrow stromal cells from different species as a preliminary approach to detect intercellular protein transfer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123657/
https://www.ncbi.nlm.nih.gov/pubmed/37092456
http://dx.doi.org/10.3390/proteomes11020015
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