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Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer
Cellular interactions within the bone marrow microenvironment modulate the properties of subsets of leukemic cells leading to the development of drug-resistant phenotypes. The intercellular transfer of proteins and organelles contributes to this process but the set of transferred proteins and their...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123657/ https://www.ncbi.nlm.nih.gov/pubmed/37092456 http://dx.doi.org/10.3390/proteomes11020015 |
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author | Nevárez-Ramírez, Abraham Josué Guzmán-Ortiz, Ana Laura Cortes-Reynosa, Pedro Perez-Salazar, Eduardo Jaimes-Ortega, Gustavo Alberto Valle-Rios, Ricardo Marín-Hernández, Álvaro Rodríguez-Zavala, José S. Ruiz-May, Eliel Castrejón-Flores, José Luis Quezada, Héctor |
author_facet | Nevárez-Ramírez, Abraham Josué Guzmán-Ortiz, Ana Laura Cortes-Reynosa, Pedro Perez-Salazar, Eduardo Jaimes-Ortega, Gustavo Alberto Valle-Rios, Ricardo Marín-Hernández, Álvaro Rodríguez-Zavala, José S. Ruiz-May, Eliel Castrejón-Flores, José Luis Quezada, Héctor |
author_sort | Nevárez-Ramírez, Abraham Josué |
collection | PubMed |
description | Cellular interactions within the bone marrow microenvironment modulate the properties of subsets of leukemic cells leading to the development of drug-resistant phenotypes. The intercellular transfer of proteins and organelles contributes to this process but the set of transferred proteins and their effects in the receiving cells remain unclear. This study aimed to detect the intercellular protein transfer from mouse bone marrow stromal cells (OP9 cell line) to human T-lymphoblasts (CCRF-CEM cell line) using nanoLC-MS/MS-based shotgun proteomics in a 3D co-culture system. After 24 h of co-culture, 1513 and 67 proteins from human and mouse origin, respectively, were identified in CCRF-CEM cells. The presence of mouse proteins in the human cell line, detected by analyzing the differences in amino acid sequences of orthologous peptides, was interpreted as the result of intercellular transfer. The transferred proteins might have contributed to the observed resistance to vincristine, methotrexate, and hydrogen peroxide in the co-cultured leukemic cells. Our results suggest that shotgun proteomic analyses of co-cultured cells from different species could be a simple option to get a preliminary survey of the proteins exchanged among interacting cells. |
format | Online Article Text |
id | pubmed-10123657 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101236572023-04-25 Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer Nevárez-Ramírez, Abraham Josué Guzmán-Ortiz, Ana Laura Cortes-Reynosa, Pedro Perez-Salazar, Eduardo Jaimes-Ortega, Gustavo Alberto Valle-Rios, Ricardo Marín-Hernández, Álvaro Rodríguez-Zavala, José S. Ruiz-May, Eliel Castrejón-Flores, José Luis Quezada, Héctor Proteomes Article Cellular interactions within the bone marrow microenvironment modulate the properties of subsets of leukemic cells leading to the development of drug-resistant phenotypes. The intercellular transfer of proteins and organelles contributes to this process but the set of transferred proteins and their effects in the receiving cells remain unclear. This study aimed to detect the intercellular protein transfer from mouse bone marrow stromal cells (OP9 cell line) to human T-lymphoblasts (CCRF-CEM cell line) using nanoLC-MS/MS-based shotgun proteomics in a 3D co-culture system. After 24 h of co-culture, 1513 and 67 proteins from human and mouse origin, respectively, were identified in CCRF-CEM cells. The presence of mouse proteins in the human cell line, detected by analyzing the differences in amino acid sequences of orthologous peptides, was interpreted as the result of intercellular transfer. The transferred proteins might have contributed to the observed resistance to vincristine, methotrexate, and hydrogen peroxide in the co-cultured leukemic cells. Our results suggest that shotgun proteomic analyses of co-cultured cells from different species could be a simple option to get a preliminary survey of the proteins exchanged among interacting cells. MDPI 2023-04-05 /pmc/articles/PMC10123657/ /pubmed/37092456 http://dx.doi.org/10.3390/proteomes11020015 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nevárez-Ramírez, Abraham Josué Guzmán-Ortiz, Ana Laura Cortes-Reynosa, Pedro Perez-Salazar, Eduardo Jaimes-Ortega, Gustavo Alberto Valle-Rios, Ricardo Marín-Hernández, Álvaro Rodríguez-Zavala, José S. Ruiz-May, Eliel Castrejón-Flores, José Luis Quezada, Héctor Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer |
title | Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer |
title_full | Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer |
title_fullStr | Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer |
title_full_unstemmed | Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer |
title_short | Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer |
title_sort | shotgun proteomics of co-cultured leukemic and bone marrow stromal cells from different species as a preliminary approach to detect intercellular protein transfer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123657/ https://www.ncbi.nlm.nih.gov/pubmed/37092456 http://dx.doi.org/10.3390/proteomes11020015 |
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