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Oxidative Stress Induced Dysfunction of Protein Synthesis in 661W Mice Photoreceptor Cells

Photoreceptor cells are highly susceptible to oxidative-stress-induced damage due to their high metabolic rate. Oxidative stress plays a key role in driving pathological events in several different ocular diseases, which lead to retinal degeneration and ultimately blindness. A growing number of stud...

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Autores principales: Deng, Liting, Gupta, Vivek, Abyadeh, Morteza, Chitranshi, Nitin, Pushpitha, Kanishka, Wu, Yunqi, Gupta, Veer, You, Yuyi, Paulo, Joao A., Graham, Stuart L., Mirzaei, Mehdi, Haynes, Paul A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123756/
https://www.ncbi.nlm.nih.gov/pubmed/37092453
http://dx.doi.org/10.3390/proteomes11020012
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author Deng, Liting
Gupta, Vivek
Abyadeh, Morteza
Chitranshi, Nitin
Pushpitha, Kanishka
Wu, Yunqi
Gupta, Veer
You, Yuyi
Paulo, Joao A.
Graham, Stuart L.
Mirzaei, Mehdi
Haynes, Paul A.
author_facet Deng, Liting
Gupta, Vivek
Abyadeh, Morteza
Chitranshi, Nitin
Pushpitha, Kanishka
Wu, Yunqi
Gupta, Veer
You, Yuyi
Paulo, Joao A.
Graham, Stuart L.
Mirzaei, Mehdi
Haynes, Paul A.
author_sort Deng, Liting
collection PubMed
description Photoreceptor cells are highly susceptible to oxidative-stress-induced damage due to their high metabolic rate. Oxidative stress plays a key role in driving pathological events in several different ocular diseases, which lead to retinal degeneration and ultimately blindness. A growing number of studies have been performed to understand downstream events caused by ROS induced oxidative stress in photoreceptor cells; however, the underlying mechanisms of ROS toxicity are not fully understood. To shed light on ROS induced downstream pathological events, we employed a tandem mass tag (TMT) labelling-based quantitative mass-spectrometric approach to determine proteome changes in 661W photoreceptor cells following oxidative stress induction via the application of different concentrations of H(2)O(2) at different time points. Overall, 5920 proteins were identified and quantified, and 450 differentially expressed proteins (DEPs) were identified, which were altered in a dose and time dependent manner in all treatment groups compared to the control group. These proteins were involved in several biological pathways, including spliceosome and ribosome response, activated glutathione metabolism, decreased ECM-receptor interaction, oxidative phosphorylation, abnormally regulated lysosome, apoptosis, and ribosome biogenesis. Our results highlighted ECM receptor interaction, oxidative phosphorylation and spliceosome pathways as the major targets of oxidative stress that might mediate vascular dysfunction and cellular senescence.
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spelling pubmed-101237562023-04-25 Oxidative Stress Induced Dysfunction of Protein Synthesis in 661W Mice Photoreceptor Cells Deng, Liting Gupta, Vivek Abyadeh, Morteza Chitranshi, Nitin Pushpitha, Kanishka Wu, Yunqi Gupta, Veer You, Yuyi Paulo, Joao A. Graham, Stuart L. Mirzaei, Mehdi Haynes, Paul A. Proteomes Article Photoreceptor cells are highly susceptible to oxidative-stress-induced damage due to their high metabolic rate. Oxidative stress plays a key role in driving pathological events in several different ocular diseases, which lead to retinal degeneration and ultimately blindness. A growing number of studies have been performed to understand downstream events caused by ROS induced oxidative stress in photoreceptor cells; however, the underlying mechanisms of ROS toxicity are not fully understood. To shed light on ROS induced downstream pathological events, we employed a tandem mass tag (TMT) labelling-based quantitative mass-spectrometric approach to determine proteome changes in 661W photoreceptor cells following oxidative stress induction via the application of different concentrations of H(2)O(2) at different time points. Overall, 5920 proteins were identified and quantified, and 450 differentially expressed proteins (DEPs) were identified, which were altered in a dose and time dependent manner in all treatment groups compared to the control group. These proteins were involved in several biological pathways, including spliceosome and ribosome response, activated glutathione metabolism, decreased ECM-receptor interaction, oxidative phosphorylation, abnormally regulated lysosome, apoptosis, and ribosome biogenesis. Our results highlighted ECM receptor interaction, oxidative phosphorylation and spliceosome pathways as the major targets of oxidative stress that might mediate vascular dysfunction and cellular senescence. MDPI 2023-04-03 /pmc/articles/PMC10123756/ /pubmed/37092453 http://dx.doi.org/10.3390/proteomes11020012 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Deng, Liting
Gupta, Vivek
Abyadeh, Morteza
Chitranshi, Nitin
Pushpitha, Kanishka
Wu, Yunqi
Gupta, Veer
You, Yuyi
Paulo, Joao A.
Graham, Stuart L.
Mirzaei, Mehdi
Haynes, Paul A.
Oxidative Stress Induced Dysfunction of Protein Synthesis in 661W Mice Photoreceptor Cells
title Oxidative Stress Induced Dysfunction of Protein Synthesis in 661W Mice Photoreceptor Cells
title_full Oxidative Stress Induced Dysfunction of Protein Synthesis in 661W Mice Photoreceptor Cells
title_fullStr Oxidative Stress Induced Dysfunction of Protein Synthesis in 661W Mice Photoreceptor Cells
title_full_unstemmed Oxidative Stress Induced Dysfunction of Protein Synthesis in 661W Mice Photoreceptor Cells
title_short Oxidative Stress Induced Dysfunction of Protein Synthesis in 661W Mice Photoreceptor Cells
title_sort oxidative stress induced dysfunction of protein synthesis in 661w mice photoreceptor cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123756/
https://www.ncbi.nlm.nih.gov/pubmed/37092453
http://dx.doi.org/10.3390/proteomes11020012
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