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In Vitro CRISPR-Cas12a-Based Detection of Cancer-Associated TP53 Hotspot Mutations Beyond the crRNA Seed Region

Cost-effective and time-efficient detection of oncogenic mutations supports improved presymptomatic cancer diagnostics and post-treatment disease monitoring. Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a is an RNA-guided endonuclease that, upon protospacer adjacent motif...

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Detalles Bibliográficos
Autores principales:	Kohabir, Kavish A.V., Nooi, Lars O., Brink, Arjen, Brakenhoff, Ruud H., Sistermans, Erik A., Wolthuis, Rob M.F.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Mary Ann Liebert, Inc., publishers 2023
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123810/ https://www.ncbi.nlm.nih.gov/pubmed/36637878 http://dx.doi.org/10.1089/crispr.2022.0077

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