Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cells

BACKGROUND: Multiple genetic modifications may be required to develop potent off-the-shelf chimeric antigen receptor (CAR) T cell therapies. Conventional CRISPR-Cas nucleases install sequence-specific DNA double-strand breaks (DSBs), enabling gene knock-out or targeted transgene knock-in. However, s...

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Detalles Bibliográficos
Autores principales: Glaser, Viktor, Flugel, Christian, Kath, Jonas, Du, Weijie, Drosdek, Vanessa, Franke, Clemens, Stein, Maik, Pruß, Axel, Schmueck-Henneresse, Michael, Volk, Hans-Dieter, Reinke, Petra, Wagner, Dimitrios L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123993/
https://www.ncbi.nlm.nih.gov/pubmed/37095570
http://dx.doi.org/10.1186/s13059-023-02928-7

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