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RT-IVT method allows multiplex real-time quantification of in vitro transcriptional mRNA production
For the past 30 years, in vitro transcription (IVT) technology has been extensively used for RNA production or for basic transcriptional mechanism research. However, methods for mRNA quantification still need to be improved. In this study, we designed a RT-IVT method using binary fluorescence quench...
Autores principales: | Zhang, Fengyu, Wang, Yipeng, Wang, Xiaomeng, Dong, Hongjie, Chen, Min, Du, Ning, Wang, Hongwei, Hu, Wei, Zhang, Kundi, Gu, Lichuan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10124930/ https://www.ncbi.nlm.nih.gov/pubmed/37095292 http://dx.doi.org/10.1038/s42003-023-04830-1 |
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