Cargando…
Monitoring Mixture Effects of Neurotoxicants in Surface Water and Wastewater Treatment Plant Effluents with Neurite Outgrowth Inhibition in SH-SY5Y Cells
[Image: see text] Cell-based assays covering environmentally relevant modes of action are widely used for water quality monitoring. However, no high-throughput assays are available for testing developmental neurotoxicity of water samples. We implemented an assay that quantifies neurite outgrowth, wh...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10125335/ https://www.ncbi.nlm.nih.gov/pubmed/37101724 http://dx.doi.org/10.1021/acsenvironau.2c00026 |
_version_ | 1785030005225422848 |
---|---|
author | Lee, Jungeun Schlichting, Rita König, Maria Scholz, Stefan Krauss, Martin Escher, Beate I. |
author_facet | Lee, Jungeun Schlichting, Rita König, Maria Scholz, Stefan Krauss, Martin Escher, Beate I. |
author_sort | Lee, Jungeun |
collection | PubMed |
description | [Image: see text] Cell-based assays covering environmentally relevant modes of action are widely used for water quality monitoring. However, no high-throughput assays are available for testing developmental neurotoxicity of water samples. We implemented an assay that quantifies neurite outgrowth, which is one of the neurodevelopmental key events, and cell viability in human neuroblastoma SH-SY5Y cells using imaging techniques. We used this assay for testing of extracts of surface water collected in agricultural areas during rain events and effluents from wastewater treatment plants (WWTPs), where more than 200 chemicals had been quantified. Forty-one chemicals were tested individually that were suspected to contribute to the mixture effects among the detected chemicals in environmental samples. Sample sensitivity distributions indicated higher neurotoxicity for surface water samples than for effluents, and the endpoint of neurite outgrowth inhibition was six times more sensitive than cytotoxicity in the surface water samples and only three times more sensitive in the effluent samples. Eight environmental pollutants showed high specificity, and those ranged from pharmaceuticals (mebendazole and verapamil) to pesticides (methiocarb and clomazone), biocides (1,2-benzisothiazolin-3-one), and industrial chemicals (N-methyl-2-pyrrolidone, 7-diethylamino-4-methylcoumarin, and 2-(4-morpholinyl)benzothiazole). Although neurotoxic effects were newly detected for some of our test chemicals, less than 1% of the measured effects were explained by the detected and toxicologically characterized chemicals. The neurotoxicity assay was benchmarked against other bioassays: activations of the aryl hydrocarbon receptor and the peroxisome proliferator-activated receptor were similar in sensitivity, highly sensitive and did not differ much between the two water types, with surface water having slightly higher effects than the WWTP effluent. Oxidative stress response mirrored neurotoxicity quite well but was caused by different chemicals in the two water types. Overall, the new cell-based neurotoxicity assay is a valuable complement to the existing battery of effect-based monitoring tools. |
format | Online Article Text |
id | pubmed-10125335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-101253352023-04-25 Monitoring Mixture Effects of Neurotoxicants in Surface Water and Wastewater Treatment Plant Effluents with Neurite Outgrowth Inhibition in SH-SY5Y Cells Lee, Jungeun Schlichting, Rita König, Maria Scholz, Stefan Krauss, Martin Escher, Beate I. ACS Environ Au [Image: see text] Cell-based assays covering environmentally relevant modes of action are widely used for water quality monitoring. However, no high-throughput assays are available for testing developmental neurotoxicity of water samples. We implemented an assay that quantifies neurite outgrowth, which is one of the neurodevelopmental key events, and cell viability in human neuroblastoma SH-SY5Y cells using imaging techniques. We used this assay for testing of extracts of surface water collected in agricultural areas during rain events and effluents from wastewater treatment plants (WWTPs), where more than 200 chemicals had been quantified. Forty-one chemicals were tested individually that were suspected to contribute to the mixture effects among the detected chemicals in environmental samples. Sample sensitivity distributions indicated higher neurotoxicity for surface water samples than for effluents, and the endpoint of neurite outgrowth inhibition was six times more sensitive than cytotoxicity in the surface water samples and only three times more sensitive in the effluent samples. Eight environmental pollutants showed high specificity, and those ranged from pharmaceuticals (mebendazole and verapamil) to pesticides (methiocarb and clomazone), biocides (1,2-benzisothiazolin-3-one), and industrial chemicals (N-methyl-2-pyrrolidone, 7-diethylamino-4-methylcoumarin, and 2-(4-morpholinyl)benzothiazole). Although neurotoxic effects were newly detected for some of our test chemicals, less than 1% of the measured effects were explained by the detected and toxicologically characterized chemicals. The neurotoxicity assay was benchmarked against other bioassays: activations of the aryl hydrocarbon receptor and the peroxisome proliferator-activated receptor were similar in sensitivity, highly sensitive and did not differ much between the two water types, with surface water having slightly higher effects than the WWTP effluent. Oxidative stress response mirrored neurotoxicity quite well but was caused by different chemicals in the two water types. Overall, the new cell-based neurotoxicity assay is a valuable complement to the existing battery of effect-based monitoring tools. American Chemical Society 2022-08-17 /pmc/articles/PMC10125335/ /pubmed/37101724 http://dx.doi.org/10.1021/acsenvironau.2c00026 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Lee, Jungeun Schlichting, Rita König, Maria Scholz, Stefan Krauss, Martin Escher, Beate I. Monitoring Mixture Effects of Neurotoxicants in Surface Water and Wastewater Treatment Plant Effluents with Neurite Outgrowth Inhibition in SH-SY5Y Cells |
title | Monitoring
Mixture Effects of Neurotoxicants in Surface
Water and Wastewater Treatment Plant Effluents with Neurite Outgrowth
Inhibition in SH-SY5Y Cells |
title_full | Monitoring
Mixture Effects of Neurotoxicants in Surface
Water and Wastewater Treatment Plant Effluents with Neurite Outgrowth
Inhibition in SH-SY5Y Cells |
title_fullStr | Monitoring
Mixture Effects of Neurotoxicants in Surface
Water and Wastewater Treatment Plant Effluents with Neurite Outgrowth
Inhibition in SH-SY5Y Cells |
title_full_unstemmed | Monitoring
Mixture Effects of Neurotoxicants in Surface
Water and Wastewater Treatment Plant Effluents with Neurite Outgrowth
Inhibition in SH-SY5Y Cells |
title_short | Monitoring
Mixture Effects of Neurotoxicants in Surface
Water and Wastewater Treatment Plant Effluents with Neurite Outgrowth
Inhibition in SH-SY5Y Cells |
title_sort | monitoring
mixture effects of neurotoxicants in surface
water and wastewater treatment plant effluents with neurite outgrowth
inhibition in sh-sy5y cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10125335/ https://www.ncbi.nlm.nih.gov/pubmed/37101724 http://dx.doi.org/10.1021/acsenvironau.2c00026 |
work_keys_str_mv | AT leejungeun monitoringmixtureeffectsofneurotoxicantsinsurfacewaterandwastewatertreatmentplanteffluentswithneuriteoutgrowthinhibitioninshsy5ycells AT schlichtingrita monitoringmixtureeffectsofneurotoxicantsinsurfacewaterandwastewatertreatmentplanteffluentswithneuriteoutgrowthinhibitioninshsy5ycells AT konigmaria monitoringmixtureeffectsofneurotoxicantsinsurfacewaterandwastewatertreatmentplanteffluentswithneuriteoutgrowthinhibitioninshsy5ycells AT scholzstefan monitoringmixtureeffectsofneurotoxicantsinsurfacewaterandwastewatertreatmentplanteffluentswithneuriteoutgrowthinhibitioninshsy5ycells AT kraussmartin monitoringmixtureeffectsofneurotoxicantsinsurfacewaterandwastewatertreatmentplanteffluentswithneuriteoutgrowthinhibitioninshsy5ycells AT escherbeatei monitoringmixtureeffectsofneurotoxicantsinsurfacewaterandwastewatertreatmentplanteffluentswithneuriteoutgrowthinhibitioninshsy5ycells |