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Evaluation of bone growth around bioactive glass S53P4 by scanning acoustic microscopy co-registered with optical interferometry and elemental analysis

Bioactive glass (BAG) is a bone substitute that can be used in orthopaedic surgery. Following implantation, the BAG is expected to be replaced by bone via bone growth and gradual degradation of the BAG. However, the hydroxyapatite mineral forming on BAG resembles bone mineral, not providing sufficie...

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Autores principales: Holmström, Axi, Meriläinen, Antti, Hyvönen, Jere, Nolvi, Anton, Ylitalo, Tuomo, Steffen, Kari, Björkenheim, Robert, Strömberg, Gustav, Nieminen, Heikki J., Kassamakov, Ivan, Pajarinen, Jukka, Hupa, Leena, Salmi, Ari, Hæggström, Edward, Lindfors, Nina C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126192/
https://www.ncbi.nlm.nih.gov/pubmed/37095138
http://dx.doi.org/10.1038/s41598-023-33454-y
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author Holmström, Axi
Meriläinen, Antti
Hyvönen, Jere
Nolvi, Anton
Ylitalo, Tuomo
Steffen, Kari
Björkenheim, Robert
Strömberg, Gustav
Nieminen, Heikki J.
Kassamakov, Ivan
Pajarinen, Jukka
Hupa, Leena
Salmi, Ari
Hæggström, Edward
Lindfors, Nina C.
author_facet Holmström, Axi
Meriläinen, Antti
Hyvönen, Jere
Nolvi, Anton
Ylitalo, Tuomo
Steffen, Kari
Björkenheim, Robert
Strömberg, Gustav
Nieminen, Heikki J.
Kassamakov, Ivan
Pajarinen, Jukka
Hupa, Leena
Salmi, Ari
Hæggström, Edward
Lindfors, Nina C.
author_sort Holmström, Axi
collection PubMed
description Bioactive glass (BAG) is a bone substitute that can be used in orthopaedic surgery. Following implantation, the BAG is expected to be replaced by bone via bone growth and gradual degradation of the BAG. However, the hydroxyapatite mineral forming on BAG resembles bone mineral, not providing sufficient contrast to distinguish the two in X-ray images. In this study, we co-registered coded-excitation scanning acoustic microscopy (CESAM), scanning white light interferometry (SWLI), and scanning electron microscopy with elemental analysis (Energy Dispersive X-ray Spectroscopy) (SEM–EDX) to investigate the bone growth and BAG reactions on a micron scale in a rabbit bone ex vivo. The acoustic impedance map recorded by the CESAM provides high elasticity-associated contrast to study materials and their combinations, while simultaneously producing a topography map of the sample. The acoustic impedance map correlated with the elemental analysis from SEM–EDX. SWLI also produces a topography map, but with higher resolution than CESAM. The two topography maps (CESAM and SWLI) were in good agreement. Furthermore, using information from both maps simultaneously produced by the CESAM (acoustic impedance and topography) allowed determining regions-of-interest related to bone formation around the BAG with greater ease than from either map alone. CESAM is therefore a promising tool for evaluating the degradation of bone substitutes and the bone healing process ex vivo.
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spelling pubmed-101261922023-04-26 Evaluation of bone growth around bioactive glass S53P4 by scanning acoustic microscopy co-registered with optical interferometry and elemental analysis Holmström, Axi Meriläinen, Antti Hyvönen, Jere Nolvi, Anton Ylitalo, Tuomo Steffen, Kari Björkenheim, Robert Strömberg, Gustav Nieminen, Heikki J. Kassamakov, Ivan Pajarinen, Jukka Hupa, Leena Salmi, Ari Hæggström, Edward Lindfors, Nina C. Sci Rep Article Bioactive glass (BAG) is a bone substitute that can be used in orthopaedic surgery. Following implantation, the BAG is expected to be replaced by bone via bone growth and gradual degradation of the BAG. However, the hydroxyapatite mineral forming on BAG resembles bone mineral, not providing sufficient contrast to distinguish the two in X-ray images. In this study, we co-registered coded-excitation scanning acoustic microscopy (CESAM), scanning white light interferometry (SWLI), and scanning electron microscopy with elemental analysis (Energy Dispersive X-ray Spectroscopy) (SEM–EDX) to investigate the bone growth and BAG reactions on a micron scale in a rabbit bone ex vivo. The acoustic impedance map recorded by the CESAM provides high elasticity-associated contrast to study materials and their combinations, while simultaneously producing a topography map of the sample. The acoustic impedance map correlated with the elemental analysis from SEM–EDX. SWLI also produces a topography map, but with higher resolution than CESAM. The two topography maps (CESAM and SWLI) were in good agreement. Furthermore, using information from both maps simultaneously produced by the CESAM (acoustic impedance and topography) allowed determining regions-of-interest related to bone formation around the BAG with greater ease than from either map alone. CESAM is therefore a promising tool for evaluating the degradation of bone substitutes and the bone healing process ex vivo. Nature Publishing Group UK 2023-04-24 /pmc/articles/PMC10126192/ /pubmed/37095138 http://dx.doi.org/10.1038/s41598-023-33454-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Holmström, Axi
Meriläinen, Antti
Hyvönen, Jere
Nolvi, Anton
Ylitalo, Tuomo
Steffen, Kari
Björkenheim, Robert
Strömberg, Gustav
Nieminen, Heikki J.
Kassamakov, Ivan
Pajarinen, Jukka
Hupa, Leena
Salmi, Ari
Hæggström, Edward
Lindfors, Nina C.
Evaluation of bone growth around bioactive glass S53P4 by scanning acoustic microscopy co-registered with optical interferometry and elemental analysis
title Evaluation of bone growth around bioactive glass S53P4 by scanning acoustic microscopy co-registered with optical interferometry and elemental analysis
title_full Evaluation of bone growth around bioactive glass S53P4 by scanning acoustic microscopy co-registered with optical interferometry and elemental analysis
title_fullStr Evaluation of bone growth around bioactive glass S53P4 by scanning acoustic microscopy co-registered with optical interferometry and elemental analysis
title_full_unstemmed Evaluation of bone growth around bioactive glass S53P4 by scanning acoustic microscopy co-registered with optical interferometry and elemental analysis
title_short Evaluation of bone growth around bioactive glass S53P4 by scanning acoustic microscopy co-registered with optical interferometry and elemental analysis
title_sort evaluation of bone growth around bioactive glass s53p4 by scanning acoustic microscopy co-registered with optical interferometry and elemental analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126192/
https://www.ncbi.nlm.nih.gov/pubmed/37095138
http://dx.doi.org/10.1038/s41598-023-33454-y
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