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Non-spike and spike-specific memory T cell responses after the third dose of inactivated COVID-19 vaccine
BACKGROUND: During the COVID-19 epidemic, vaccination has become the most safe and effective way to prevent severe illness and death. Inactivated vaccines are the most widely used type of COVID-19 vaccines in the world. In contrast to spike-based mRNA/protein COVID-19 vaccines, inactivated vaccines...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126277/ https://www.ncbi.nlm.nih.gov/pubmed/37114058 http://dx.doi.org/10.3389/fimmu.2023.1139620 |
Sumario: | BACKGROUND: During the COVID-19 epidemic, vaccination has become the most safe and effective way to prevent severe illness and death. Inactivated vaccines are the most widely used type of COVID-19 vaccines in the world. In contrast to spike-based mRNA/protein COVID-19 vaccines, inactivated vaccines generate antibodies and T cell responses against both spike and non-spike antigens. However, the knowledge of inactivated vaccines in inducing non-spike-specific T cell response is very limited. METHODS: In this study, eighteen healthcare volunteers received a homogenous booster (third) dose of the CoronaVac vaccine at least 6 months after the second dose. CD4(+) and CD8(+) T cell responses against a peptide pool from wild-type (WT) non-spike proteins and spike peptide pools from WT, Delta, and Omicron SARS-CoV-2 were examined before and 1-2 weeks after the booster dose. RESULTS: The booster dose elevated cytokine response in CD4(+) and CD8(+) T cells as well as expression of cytotoxic marker CD107a in CD8(+) T cells in response to non-spike and spike antigens. The frequencies of cytokine-secreting non-spike-specific CD4(+) and CD8(+) T cells correlated well with those of spike-specific from WT, Delta, and Omicron. Activation-induced markers (AIM) assay also revealed that booster vaccination elicited non-spike-specific CD4(+) and CD8(+) T cell responses. In addition, booster vaccination produced similar spike-specific AIM(+)CD4(+) and AIM(+)CD8(+) T cell responses to WT, Delta, and Omicron, indicting strong cross-reactivity of functional cellular response between WT and variants. Furthermore, booster vaccination induced effector memory phenotypes of spike-specific and non-spike-specific CD4(+) and CD8(+) T cells. CONCLUSIONS: These data suggest that the booster dose of inactive vaccines broadens both non-spike-specific and spike-specific T cell responses against SARS-CoV-2. |
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