Cargando…

Detection of antimicrobial producing Staphylococcus from migratory birds: Potential role in nasotracheal microbiota modulation

A collection of 259 staphylococci of 13 different species [212 coagulase-negative (CoNS) and 47 coagulase-positive (CoPS)] recovered from nasotracheal samples of 87 healthy nestling white storks was tested by the spot-on-lawn method for antimicrobial-activity (AA) against 14 indicator bacteria. More...

Descripción completa

Detalles Bibliográficos
Autores principales: Fernández-Fernández, Rosa, Abdullahi, Idris Nasir, González-Azcona, Carmen, Ulloa, Adriana, Martínez, Agustí, García-Vela, Sara, Höfle, Ursula, Zarazaga, Myriam, Lozano, Carmen, Torres, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126283/
https://www.ncbi.nlm.nih.gov/pubmed/37113241
http://dx.doi.org/10.3389/fmicb.2023.1144975
_version_ 1785030206649532416
author Fernández-Fernández, Rosa
Abdullahi, Idris Nasir
González-Azcona, Carmen
Ulloa, Adriana
Martínez, Agustí
García-Vela, Sara
Höfle, Ursula
Zarazaga, Myriam
Lozano, Carmen
Torres, Carmen
author_facet Fernández-Fernández, Rosa
Abdullahi, Idris Nasir
González-Azcona, Carmen
Ulloa, Adriana
Martínez, Agustí
García-Vela, Sara
Höfle, Ursula
Zarazaga, Myriam
Lozano, Carmen
Torres, Carmen
author_sort Fernández-Fernández, Rosa
collection PubMed
description A collection of 259 staphylococci of 13 different species [212 coagulase-negative (CoNS) and 47 coagulase-positive (CoPS)] recovered from nasotracheal samples of 87 healthy nestling white storks was tested by the spot-on-lawn method for antimicrobial-activity (AA) against 14 indicator bacteria. Moreover, extracts of AP isolates were obtained [cell-free-supernatants (CFS) both crude and concentrated and butanol extracts] and tested against the 14 indicator bacteria. The microbiota modulation capacity of AP isolates was tested considering: (a) intra-sample AA, against all Gram-positive bacteria recovered in the same stork nasotracheal sample; (b) inter-sample AA against a selection of representative Gram-positive bacteria of the nasotracheal microbiota of all the storks (30 isolates of 29 different species and nine genera). In addition, enzymatic susceptibility test was carried out in selected AP isolates and bacteriocin encoding genes was studied by PCR/sequencing. In this respect, nine isolates (3.5%; seven CoNS and two CoPS) showed AA against at least one indicator bacteria and were considered antimicrobial-producing (AP) isolates. The AP isolates showed AA only for Gram-positive bacteria. Three of these AP isolates (S. hominis X3764, S. sciuri X4000, and S. chromogenes X4620) revealed AA on all extract conditions; other four AP isolates only showed activity in extracts after concentration; the remaining two AP isolates did not show AA in any of extract conditions. As for the microbiota modulation evaluation, three of the nine AP-isolates revealed intra-sample AA. It is to highlight the potent inter-sample AA of the X3764 isolate inhibiting 73% of the 29 representative Gram-positive species of the nasotracheal stork microbiota population. On the other hand, enzymatic analysis carried out in the two highest AP isolates (X3764 and X4000) verified the proteinaceous nature of the antimicrobial compound and PCR analysis revealed the presence of lantibiotic-like encoding genes in the nine AP isolates. In conclusion, these results show that nasotracheal staphylococci of healthy storks, and especially CoNS, produce antimicrobial substances that could be important in the modulations of their nasal microbiota.
format Online
Article
Text
id pubmed-10126283
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-101262832023-04-26 Detection of antimicrobial producing Staphylococcus from migratory birds: Potential role in nasotracheal microbiota modulation Fernández-Fernández, Rosa Abdullahi, Idris Nasir González-Azcona, Carmen Ulloa, Adriana Martínez, Agustí García-Vela, Sara Höfle, Ursula Zarazaga, Myriam Lozano, Carmen Torres, Carmen Front Microbiol Microbiology A collection of 259 staphylococci of 13 different species [212 coagulase-negative (CoNS) and 47 coagulase-positive (CoPS)] recovered from nasotracheal samples of 87 healthy nestling white storks was tested by the spot-on-lawn method for antimicrobial-activity (AA) against 14 indicator bacteria. Moreover, extracts of AP isolates were obtained [cell-free-supernatants (CFS) both crude and concentrated and butanol extracts] and tested against the 14 indicator bacteria. The microbiota modulation capacity of AP isolates was tested considering: (a) intra-sample AA, against all Gram-positive bacteria recovered in the same stork nasotracheal sample; (b) inter-sample AA against a selection of representative Gram-positive bacteria of the nasotracheal microbiota of all the storks (30 isolates of 29 different species and nine genera). In addition, enzymatic susceptibility test was carried out in selected AP isolates and bacteriocin encoding genes was studied by PCR/sequencing. In this respect, nine isolates (3.5%; seven CoNS and two CoPS) showed AA against at least one indicator bacteria and were considered antimicrobial-producing (AP) isolates. The AP isolates showed AA only for Gram-positive bacteria. Three of these AP isolates (S. hominis X3764, S. sciuri X4000, and S. chromogenes X4620) revealed AA on all extract conditions; other four AP isolates only showed activity in extracts after concentration; the remaining two AP isolates did not show AA in any of extract conditions. As for the microbiota modulation evaluation, three of the nine AP-isolates revealed intra-sample AA. It is to highlight the potent inter-sample AA of the X3764 isolate inhibiting 73% of the 29 representative Gram-positive species of the nasotracheal stork microbiota population. On the other hand, enzymatic analysis carried out in the two highest AP isolates (X3764 and X4000) verified the proteinaceous nature of the antimicrobial compound and PCR analysis revealed the presence of lantibiotic-like encoding genes in the nine AP isolates. In conclusion, these results show that nasotracheal staphylococci of healthy storks, and especially CoNS, produce antimicrobial substances that could be important in the modulations of their nasal microbiota. Frontiers Media S.A. 2023-04-11 /pmc/articles/PMC10126283/ /pubmed/37113241 http://dx.doi.org/10.3389/fmicb.2023.1144975 Text en Copyright © 2023 Fernández-Fernández, Abdullahi, González-Azcona, Ulloa, Martínez, García-Vela, Höfle, Zarazaga, Lozano and Torres. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Fernández-Fernández, Rosa
Abdullahi, Idris Nasir
González-Azcona, Carmen
Ulloa, Adriana
Martínez, Agustí
García-Vela, Sara
Höfle, Ursula
Zarazaga, Myriam
Lozano, Carmen
Torres, Carmen
Detection of antimicrobial producing Staphylococcus from migratory birds: Potential role in nasotracheal microbiota modulation
title Detection of antimicrobial producing Staphylococcus from migratory birds: Potential role in nasotracheal microbiota modulation
title_full Detection of antimicrobial producing Staphylococcus from migratory birds: Potential role in nasotracheal microbiota modulation
title_fullStr Detection of antimicrobial producing Staphylococcus from migratory birds: Potential role in nasotracheal microbiota modulation
title_full_unstemmed Detection of antimicrobial producing Staphylococcus from migratory birds: Potential role in nasotracheal microbiota modulation
title_short Detection of antimicrobial producing Staphylococcus from migratory birds: Potential role in nasotracheal microbiota modulation
title_sort detection of antimicrobial producing staphylococcus from migratory birds: potential role in nasotracheal microbiota modulation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126283/
https://www.ncbi.nlm.nih.gov/pubmed/37113241
http://dx.doi.org/10.3389/fmicb.2023.1144975
work_keys_str_mv AT fernandezfernandezrosa detectionofantimicrobialproducingstaphylococcusfrommigratorybirdspotentialroleinnasotrachealmicrobiotamodulation
AT abdullahiidrisnasir detectionofantimicrobialproducingstaphylococcusfrommigratorybirdspotentialroleinnasotrachealmicrobiotamodulation
AT gonzalezazconacarmen detectionofantimicrobialproducingstaphylococcusfrommigratorybirdspotentialroleinnasotrachealmicrobiotamodulation
AT ulloaadriana detectionofantimicrobialproducingstaphylococcusfrommigratorybirdspotentialroleinnasotrachealmicrobiotamodulation
AT martinezagusti detectionofantimicrobialproducingstaphylococcusfrommigratorybirdspotentialroleinnasotrachealmicrobiotamodulation
AT garciavelasara detectionofantimicrobialproducingstaphylococcusfrommigratorybirdspotentialroleinnasotrachealmicrobiotamodulation
AT hofleursula detectionofantimicrobialproducingstaphylococcusfrommigratorybirdspotentialroleinnasotrachealmicrobiotamodulation
AT zarazagamyriam detectionofantimicrobialproducingstaphylococcusfrommigratorybirdspotentialroleinnasotrachealmicrobiotamodulation
AT lozanocarmen detectionofantimicrobialproducingstaphylococcusfrommigratorybirdspotentialroleinnasotrachealmicrobiotamodulation
AT torrescarmen detectionofantimicrobialproducingstaphylococcusfrommigratorybirdspotentialroleinnasotrachealmicrobiotamodulation