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Hydroxyanthracene derivates citotoxicity: A differential evaluation between single molecule and whole plant extract

Hydroxyanthracene derivates (HADs) are a group of natural or synthetic compounds with a wide range of biological activities (for instance, anti-inflammatory, antibacterial, and antiarthritic). In addition, because of their properties for helping the normal bowel function, HADs are widely used in con...

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Autores principales: Tinti, Laura, Cicaloni, Vittoria, Nezi, Paola, Isoldi, Giovanni, Etiope, Paolo, Barlozzini, Barbara, Pecorari, Rita, Salvini, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126332/
https://www.ncbi.nlm.nih.gov/pubmed/37113593
http://dx.doi.org/10.3389/fpls.2023.1166075
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author Tinti, Laura
Cicaloni, Vittoria
Nezi, Paola
Isoldi, Giovanni
Etiope, Paolo
Barlozzini, Barbara
Pecorari, Rita
Salvini, Laura
author_facet Tinti, Laura
Cicaloni, Vittoria
Nezi, Paola
Isoldi, Giovanni
Etiope, Paolo
Barlozzini, Barbara
Pecorari, Rita
Salvini, Laura
author_sort Tinti, Laura
collection PubMed
description Hydroxyanthracene derivates (HADs) are a group of natural or synthetic compounds with a wide range of biological activities (for instance, anti-inflammatory, antibacterial, and antiarthritic). In addition, because of their properties for helping the normal bowel function, HADs are widely used in constipation as pharmacological drugs and nutritional supplements. Nevertheless, during the past years, a safety usage of HAD products has been under consideration because some studies reported that HADs are not lacking toxicity (i.e., genotoxic and carcinogenic activity). Thus, the first objective of this study is to shed light on the large variability in composition of botanical food supplements containing HAD by a systematic analysis of the qualitative and quantitative composition of a cohort of extracts and raw materials of plants with high levels of anthraquinones commercially available (Cassia angustifolia, Rhamnus purshiana, Rhamnus frangula, Rheum palmatum, and Rheum raponticum). To date, the investigation of HAD toxicity was based on in vitro and in vivo studies conducted mainly on the use of the single molecules (emodin, aloe-emodin, and rhein) rather than on the whole plant extract. The qualitative-quantitative characterization was the starting point to select the most appropriate products to be used as treatment for our in vitro cell studies. Thus, the second objective of this study is the investigation, for the first time, of the toxic events of HAD used as single molecule in comparison with the whole plant extracts containing HAD in an intestinal in vitro model using human colorectal adenocarcinoma cells (Caco-2). In addition, a shotgun proteomics approach was applied to profile the differential protein expression in the Caco-2 cells after a single-HAD or whole–plant extract treatment to fully understand the potential targets and signaling pathways. In conclusion, the combination of a detailed phytochemical characterization of HAD products and a largely accurate analysis of the proteomic profile of intestinal cells treated with HAD products provided the opportunity to investigate their effects in the intestinal system.
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spelling pubmed-101263322023-04-26 Hydroxyanthracene derivates citotoxicity: A differential evaluation between single molecule and whole plant extract Tinti, Laura Cicaloni, Vittoria Nezi, Paola Isoldi, Giovanni Etiope, Paolo Barlozzini, Barbara Pecorari, Rita Salvini, Laura Front Plant Sci Plant Science Hydroxyanthracene derivates (HADs) are a group of natural or synthetic compounds with a wide range of biological activities (for instance, anti-inflammatory, antibacterial, and antiarthritic). In addition, because of their properties for helping the normal bowel function, HADs are widely used in constipation as pharmacological drugs and nutritional supplements. Nevertheless, during the past years, a safety usage of HAD products has been under consideration because some studies reported that HADs are not lacking toxicity (i.e., genotoxic and carcinogenic activity). Thus, the first objective of this study is to shed light on the large variability in composition of botanical food supplements containing HAD by a systematic analysis of the qualitative and quantitative composition of a cohort of extracts and raw materials of plants with high levels of anthraquinones commercially available (Cassia angustifolia, Rhamnus purshiana, Rhamnus frangula, Rheum palmatum, and Rheum raponticum). To date, the investigation of HAD toxicity was based on in vitro and in vivo studies conducted mainly on the use of the single molecules (emodin, aloe-emodin, and rhein) rather than on the whole plant extract. The qualitative-quantitative characterization was the starting point to select the most appropriate products to be used as treatment for our in vitro cell studies. Thus, the second objective of this study is the investigation, for the first time, of the toxic events of HAD used as single molecule in comparison with the whole plant extracts containing HAD in an intestinal in vitro model using human colorectal adenocarcinoma cells (Caco-2). In addition, a shotgun proteomics approach was applied to profile the differential protein expression in the Caco-2 cells after a single-HAD or whole–plant extract treatment to fully understand the potential targets and signaling pathways. In conclusion, the combination of a detailed phytochemical characterization of HAD products and a largely accurate analysis of the proteomic profile of intestinal cells treated with HAD products provided the opportunity to investigate their effects in the intestinal system. Frontiers Media S.A. 2023-04-11 /pmc/articles/PMC10126332/ /pubmed/37113593 http://dx.doi.org/10.3389/fpls.2023.1166075 Text en Copyright © 2023 Tinti, Cicaloni, Nezi, Isoldi, Etiope, Barlozzini, Pecorari and Salvini https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Tinti, Laura
Cicaloni, Vittoria
Nezi, Paola
Isoldi, Giovanni
Etiope, Paolo
Barlozzini, Barbara
Pecorari, Rita
Salvini, Laura
Hydroxyanthracene derivates citotoxicity: A differential evaluation between single molecule and whole plant extract
title Hydroxyanthracene derivates citotoxicity: A differential evaluation between single molecule and whole plant extract
title_full Hydroxyanthracene derivates citotoxicity: A differential evaluation between single molecule and whole plant extract
title_fullStr Hydroxyanthracene derivates citotoxicity: A differential evaluation between single molecule and whole plant extract
title_full_unstemmed Hydroxyanthracene derivates citotoxicity: A differential evaluation between single molecule and whole plant extract
title_short Hydroxyanthracene derivates citotoxicity: A differential evaluation between single molecule and whole plant extract
title_sort hydroxyanthracene derivates citotoxicity: a differential evaluation between single molecule and whole plant extract
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126332/
https://www.ncbi.nlm.nih.gov/pubmed/37113593
http://dx.doi.org/10.3389/fpls.2023.1166075
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