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Phylogeography of Francisella tularensis subspecies holarctica and epidemiology of tularemia in Switzerland
Tularemia, an endemic disease that mainly affects wild animals and humans, is caused by Francisella tularensis subsp. holarctica (Fth) in Switzerland. The Swiss Fth population consist of multiple different subclades which are distributed throughout the country. The aim of this study is to characteri...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126411/ https://www.ncbi.nlm.nih.gov/pubmed/37113234 http://dx.doi.org/10.3389/fmicb.2023.1151049 |
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author | Schütz, Sara Doina Liechti, Nicole Altpeter, Ekkehardt Labutin, Anton Wütrich, Tsering Schmidt, Kristina Maria Buettcher, Michael Moser, Michel Bruggmann, Rémy Wittwer, Matthias |
author_facet | Schütz, Sara Doina Liechti, Nicole Altpeter, Ekkehardt Labutin, Anton Wütrich, Tsering Schmidt, Kristina Maria Buettcher, Michael Moser, Michel Bruggmann, Rémy Wittwer, Matthias |
author_sort | Schütz, Sara Doina |
collection | PubMed |
description | Tularemia, an endemic disease that mainly affects wild animals and humans, is caused by Francisella tularensis subsp. holarctica (Fth) in Switzerland. The Swiss Fth population consist of multiple different subclades which are distributed throughout the country. The aim of this study is to characterize the genetic diversity of Fth in Switzerland and to describe the phylogeographic relationship of isolates by single nucleotide polymorphism (SNP) analysis. This analysis is combined with human surveillance data from reported cases over the last 10 years and in vitro and in silico antibiotic resistance tests to provide insight into the epidemiology of tularemia in Switzerland. We sequenced the whole genomes of 52 Fth strains of human or tick origin collected in Switzerland between 2009 and 2022 and analyzed together with all publicly available sequencing data of Swiss and European Fth. Next, we performed a preliminary classification with the established canonical single nucleotide polymorphism nomenclature. Furthermore, we tested 20 isolates from all main Swiss clades for antimicrobial susceptibility against a panel of antimicrobial agents. All 52 sequenced isolates from Switzerland belong to major clade B.6, specifically subclades B.45 and B.46, previously described in Western Europe. We were able to accurately reconstruct the population structure according to the global phylogenetic framework. No resistance to clinically recommended antibiotics could be identified in vitro or in silico in the western B.6 strains. |
format | Online Article Text |
id | pubmed-10126411 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101264112023-04-26 Phylogeography of Francisella tularensis subspecies holarctica and epidemiology of tularemia in Switzerland Schütz, Sara Doina Liechti, Nicole Altpeter, Ekkehardt Labutin, Anton Wütrich, Tsering Schmidt, Kristina Maria Buettcher, Michael Moser, Michel Bruggmann, Rémy Wittwer, Matthias Front Microbiol Microbiology Tularemia, an endemic disease that mainly affects wild animals and humans, is caused by Francisella tularensis subsp. holarctica (Fth) in Switzerland. The Swiss Fth population consist of multiple different subclades which are distributed throughout the country. The aim of this study is to characterize the genetic diversity of Fth in Switzerland and to describe the phylogeographic relationship of isolates by single nucleotide polymorphism (SNP) analysis. This analysis is combined with human surveillance data from reported cases over the last 10 years and in vitro and in silico antibiotic resistance tests to provide insight into the epidemiology of tularemia in Switzerland. We sequenced the whole genomes of 52 Fth strains of human or tick origin collected in Switzerland between 2009 and 2022 and analyzed together with all publicly available sequencing data of Swiss and European Fth. Next, we performed a preliminary classification with the established canonical single nucleotide polymorphism nomenclature. Furthermore, we tested 20 isolates from all main Swiss clades for antimicrobial susceptibility against a panel of antimicrobial agents. All 52 sequenced isolates from Switzerland belong to major clade B.6, specifically subclades B.45 and B.46, previously described in Western Europe. We were able to accurately reconstruct the population structure according to the global phylogenetic framework. No resistance to clinically recommended antibiotics could be identified in vitro or in silico in the western B.6 strains. Frontiers Media S.A. 2023-04-11 /pmc/articles/PMC10126411/ /pubmed/37113234 http://dx.doi.org/10.3389/fmicb.2023.1151049 Text en Copyright © 2023 Schütz, Liechti, Altpeter, Labutin, Wütrich, Schmidt, Buettcher, Moser, Bruggmann and Wittwer. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Schütz, Sara Doina Liechti, Nicole Altpeter, Ekkehardt Labutin, Anton Wütrich, Tsering Schmidt, Kristina Maria Buettcher, Michael Moser, Michel Bruggmann, Rémy Wittwer, Matthias Phylogeography of Francisella tularensis subspecies holarctica and epidemiology of tularemia in Switzerland |
title | Phylogeography of Francisella tularensis subspecies holarctica and epidemiology of tularemia in Switzerland |
title_full | Phylogeography of Francisella tularensis subspecies holarctica and epidemiology of tularemia in Switzerland |
title_fullStr | Phylogeography of Francisella tularensis subspecies holarctica and epidemiology of tularemia in Switzerland |
title_full_unstemmed | Phylogeography of Francisella tularensis subspecies holarctica and epidemiology of tularemia in Switzerland |
title_short | Phylogeography of Francisella tularensis subspecies holarctica and epidemiology of tularemia in Switzerland |
title_sort | phylogeography of francisella tularensis subspecies holarctica and epidemiology of tularemia in switzerland |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126411/ https://www.ncbi.nlm.nih.gov/pubmed/37113234 http://dx.doi.org/10.3389/fmicb.2023.1151049 |
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