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Engraftment of Kidney Organoids In Vivo
PURPOSE OF REVIEW: Kidney organoids are heterocellular structures grown in vitro that resemble nephrons. Organoids contain diverse cell types, including podocytes, proximal tubules, and distal tubules in contiguous segments, patterned along a proximal-to-distal axis. Human organoids are being explor...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126570/ https://www.ncbi.nlm.nih.gov/pubmed/37128257 http://dx.doi.org/10.1007/s40472-023-00397-2 |
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author | Freedman, Benjamin S. Dekel, Benjamin |
author_facet | Freedman, Benjamin S. Dekel, Benjamin |
author_sort | Freedman, Benjamin S. |
collection | PubMed |
description | PURPOSE OF REVIEW: Kidney organoids are heterocellular structures grown in vitro that resemble nephrons. Organoids contain diverse cell types, including podocytes, proximal tubules, and distal tubules in contiguous segments, patterned along a proximal-to-distal axis. Human organoids are being explored for their potential as regenerative grafts, as an alternative to allograft transplants and hemodialysis. Earlier work, analyzing grafts of developing human kidney tissue and whole human embryonic kidney rudiments, serves as a baseline for organoid implantation experiments. RECENT FINDINGS: When transplanted into immunodeficient mice beneath the kidney capsule, kidney organoid xenografts can form vascularized, glomerulus-like structures, which exhibit a degree of filtration function. However, the absence of an appropriate collecting duct outlet and the presence of abundant stromal-like cells limits the functionality of such grafts and raises safety concerns. Recently, ureteric-like organoids have also been generated, which extend projections that resemble collecting ducts. SUMMARY: Combining nephron-like and ureteric-like organoids, along with renal stromal cells, may provide a path towards more functional grafts. |
format | Online Article Text |
id | pubmed-10126570 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-101265702023-04-27 Engraftment of Kidney Organoids In Vivo Freedman, Benjamin S. Dekel, Benjamin Curr Transplant Rep Article PURPOSE OF REVIEW: Kidney organoids are heterocellular structures grown in vitro that resemble nephrons. Organoids contain diverse cell types, including podocytes, proximal tubules, and distal tubules in contiguous segments, patterned along a proximal-to-distal axis. Human organoids are being explored for their potential as regenerative grafts, as an alternative to allograft transplants and hemodialysis. Earlier work, analyzing grafts of developing human kidney tissue and whole human embryonic kidney rudiments, serves as a baseline for organoid implantation experiments. RECENT FINDINGS: When transplanted into immunodeficient mice beneath the kidney capsule, kidney organoid xenografts can form vascularized, glomerulus-like structures, which exhibit a degree of filtration function. However, the absence of an appropriate collecting duct outlet and the presence of abundant stromal-like cells limits the functionality of such grafts and raises safety concerns. Recently, ureteric-like organoids have also been generated, which extend projections that resemble collecting ducts. SUMMARY: Combining nephron-like and ureteric-like organoids, along with renal stromal cells, may provide a path towards more functional grafts. Springer International Publishing 2023-04-25 2023 /pmc/articles/PMC10126570/ /pubmed/37128257 http://dx.doi.org/10.1007/s40472-023-00397-2 Text en © The Author(s), under exclusive licence to Springer Nature Switzerland AG 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Freedman, Benjamin S. Dekel, Benjamin Engraftment of Kidney Organoids In Vivo |
title | Engraftment of Kidney Organoids In Vivo |
title_full | Engraftment of Kidney Organoids In Vivo |
title_fullStr | Engraftment of Kidney Organoids In Vivo |
title_full_unstemmed | Engraftment of Kidney Organoids In Vivo |
title_short | Engraftment of Kidney Organoids In Vivo |
title_sort | engraftment of kidney organoids in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126570/ https://www.ncbi.nlm.nih.gov/pubmed/37128257 http://dx.doi.org/10.1007/s40472-023-00397-2 |
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