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Correlation between caries activity and salivary microbiota in preschool children

Early childhood caries (ECC) is the most common chronic infectious oral disease in preschool children worldwide. It is closely related to the caries activity (CA) of children. However, the distribution characteristics of oral saliva microbiomes in children with different CA are largely underexplored...

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Autores principales: Lin, Xiuyan, Wang, Yuan, Ma, Zhe, Xie, Meng, Liu, Zhuo, Cheng, Jinghui, Tian, Yuzhao, Shi, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126836/
https://www.ncbi.nlm.nih.gov/pubmed/37113131
http://dx.doi.org/10.3389/fcimb.2023.1141474
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author Lin, Xiuyan
Wang, Yuan
Ma, Zhe
Xie, Meng
Liu, Zhuo
Cheng, Jinghui
Tian, Yuzhao
Shi, Hong
author_facet Lin, Xiuyan
Wang, Yuan
Ma, Zhe
Xie, Meng
Liu, Zhuo
Cheng, Jinghui
Tian, Yuzhao
Shi, Hong
author_sort Lin, Xiuyan
collection PubMed
description Early childhood caries (ECC) is the most common chronic infectious oral disease in preschool children worldwide. It is closely related to the caries activity (CA) of children. However, the distribution characteristics of oral saliva microbiomes in children with different CA are largely underexplored. The aim of this study was to investigate the microbial community in saliva of preschool children with different CA and caries status, and to analyze the difference of microbial community in saliva of children with different CA and its correlation with ECC. Subjects were divided into 3 groups based on the Cariostat caries activity test: Group H, high CA (n=30); Group M, medium CA (n = 30); Group L, low CA (n=30). Questionnaire survey was used to explore the related influencing factors of CA. According to the caries status (on the basis of decayed mising filled teeth), these subjects were divided into caries-free group (dmft=0, n=19), caries-low group (0 < dmft ≤ 4, n=27) and caries-high group (dmft > 4, n=44). Microbial profiles of oral saliva were analyzed using 16S rRNA gene sequencing. There were significant differences in the microbial structure (P < 0.05). Scardovia and Selenomonas were the biomarkers of both H group and high caries group. The genus Abiotrophia and Lautropia were the biomarkers of both the L group and the low caries group, while the Lactobacillus and Arthrospira spp. were significantly enriched in the M group. The area under the ROC curve of the combined application of dmft score, age, frequency of sugary beverage intake, and the genus Scardovia, Selenomonas, and Campylobacter in screening children with high CA was 0.842. Moreover, function prediction using the MetaCyc database showed that there were significant differences in 11 metabolic pathways of salivary microbiota among different CA groups. Certain bacteria genera in saliva such as Scardovia and Selenomonas may be helpful in screening children with high CA.
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spelling pubmed-101268362023-04-26 Correlation between caries activity and salivary microbiota in preschool children Lin, Xiuyan Wang, Yuan Ma, Zhe Xie, Meng Liu, Zhuo Cheng, Jinghui Tian, Yuzhao Shi, Hong Front Cell Infect Microbiol Cellular and Infection Microbiology Early childhood caries (ECC) is the most common chronic infectious oral disease in preschool children worldwide. It is closely related to the caries activity (CA) of children. However, the distribution characteristics of oral saliva microbiomes in children with different CA are largely underexplored. The aim of this study was to investigate the microbial community in saliva of preschool children with different CA and caries status, and to analyze the difference of microbial community in saliva of children with different CA and its correlation with ECC. Subjects were divided into 3 groups based on the Cariostat caries activity test: Group H, high CA (n=30); Group M, medium CA (n = 30); Group L, low CA (n=30). Questionnaire survey was used to explore the related influencing factors of CA. According to the caries status (on the basis of decayed mising filled teeth), these subjects were divided into caries-free group (dmft=0, n=19), caries-low group (0 < dmft ≤ 4, n=27) and caries-high group (dmft > 4, n=44). Microbial profiles of oral saliva were analyzed using 16S rRNA gene sequencing. There were significant differences in the microbial structure (P < 0.05). Scardovia and Selenomonas were the biomarkers of both H group and high caries group. The genus Abiotrophia and Lautropia were the biomarkers of both the L group and the low caries group, while the Lactobacillus and Arthrospira spp. were significantly enriched in the M group. The area under the ROC curve of the combined application of dmft score, age, frequency of sugary beverage intake, and the genus Scardovia, Selenomonas, and Campylobacter in screening children with high CA was 0.842. Moreover, function prediction using the MetaCyc database showed that there were significant differences in 11 metabolic pathways of salivary microbiota among different CA groups. Certain bacteria genera in saliva such as Scardovia and Selenomonas may be helpful in screening children with high CA. Frontiers Media S.A. 2023-04-11 /pmc/articles/PMC10126836/ /pubmed/37113131 http://dx.doi.org/10.3389/fcimb.2023.1141474 Text en Copyright © 2023 Lin, Wang, Ma, Xie, Liu, Cheng, Tian and Shi https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Lin, Xiuyan
Wang, Yuan
Ma, Zhe
Xie, Meng
Liu, Zhuo
Cheng, Jinghui
Tian, Yuzhao
Shi, Hong
Correlation between caries activity and salivary microbiota in preschool children
title Correlation between caries activity and salivary microbiota in preschool children
title_full Correlation between caries activity and salivary microbiota in preschool children
title_fullStr Correlation between caries activity and salivary microbiota in preschool children
title_full_unstemmed Correlation between caries activity and salivary microbiota in preschool children
title_short Correlation between caries activity and salivary microbiota in preschool children
title_sort correlation between caries activity and salivary microbiota in preschool children
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126836/
https://www.ncbi.nlm.nih.gov/pubmed/37113131
http://dx.doi.org/10.3389/fcimb.2023.1141474
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