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Concordance of adenosine deaminase with immunoglobulins and lymphocyte subsets in EBV-related diseases
BACKGROUND: Clinical manifestations of Epstein–Barr virus (EBV) infection are diverse. This study aimed to explore the immune response in EBV-related diseases and the correlation between immune cells and adenosine deaminase (ADA) levels. METHODS: This study was conducted at the Children’s Hospital o...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127006/ https://www.ncbi.nlm.nih.gov/pubmed/37095577 http://dx.doi.org/10.1186/s13052-023-01457-0 |
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author | Shi, Ting Ding, Qi Liu, Xinglou Ai, Guo Zhou, Hua Huang, Linlin |
author_facet | Shi, Ting Ding, Qi Liu, Xinglou Ai, Guo Zhou, Hua Huang, Linlin |
author_sort | Shi, Ting |
collection | PubMed |
description | BACKGROUND: Clinical manifestations of Epstein–Barr virus (EBV) infection are diverse. This study aimed to explore the immune response in EBV-related diseases and the correlation between immune cells and adenosine deaminase (ADA) levels. METHODS: This study was conducted at the Children’s Hospital of Soochow University. In total, 104 patients with EBV-associated respiratory tract infection (EBV-RTI), 32 patients with atypical EBV infection, 54 patients with EBV-associated infectious mononucleosis (IM1, with normal alanine aminotransferase [ALT] levels), 50 patients with EBV-IM2 (with elevated ALT levels), 50 patients with acute respiratory infection (AURI, with other pathogens), and 30 healthy controls were enrolled in this study. Indicators of ADA, immunoglobulins (Igs), and lymphocyte subsets were analyzed for EBV-related diseases. RESULTS: Differences in the white blood cell, lymphocyte counts, ADA levels, IgA, IgG and IgM titers, percentage of CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), CD16(+)CD56(+), CD3(−)CD19(+), and CD19(+)CD23(+) lymphocytes, and CD4(+)/CD8(+) ratio between EBV-related disease groups were all statistically significant (P < 0.01). ADA levels in the EBV-related disease groups were significantly higher than those in the control group (P < 0.01). The lymphocyte count, ADA levels, IgA and IgG titers, and percentage of CD3(+) and CD3(+)CD8 + lymphocytes in the atypical EBV infection, EBV-IM1, and EBV-IM2 groups were significantly higher than those in the EBV-RTI, AUTI, and control groups (P < 0.01), whereas the percentage of CD3(+)CD4(+), CD3(−)CD19(+), and CD19(+)CD23(+) lymphocytes and CD4(+)/CD8(+) ratio showed the opposite trend. ADA levels were consistent with and closely related to the viral load and cellular and humoral immunity in EBV-related diseases. CONCLUSIONS: ADA levels, humoral immunity, and cellular immunity were diverse in EBV-related diseases, and ADA was closely related to Igs and lymphocyte subsets. |
format | Online Article Text |
id | pubmed-10127006 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-101270062023-04-26 Concordance of adenosine deaminase with immunoglobulins and lymphocyte subsets in EBV-related diseases Shi, Ting Ding, Qi Liu, Xinglou Ai, Guo Zhou, Hua Huang, Linlin Ital J Pediatr Research BACKGROUND: Clinical manifestations of Epstein–Barr virus (EBV) infection are diverse. This study aimed to explore the immune response in EBV-related diseases and the correlation between immune cells and adenosine deaminase (ADA) levels. METHODS: This study was conducted at the Children’s Hospital of Soochow University. In total, 104 patients with EBV-associated respiratory tract infection (EBV-RTI), 32 patients with atypical EBV infection, 54 patients with EBV-associated infectious mononucleosis (IM1, with normal alanine aminotransferase [ALT] levels), 50 patients with EBV-IM2 (with elevated ALT levels), 50 patients with acute respiratory infection (AURI, with other pathogens), and 30 healthy controls were enrolled in this study. Indicators of ADA, immunoglobulins (Igs), and lymphocyte subsets were analyzed for EBV-related diseases. RESULTS: Differences in the white blood cell, lymphocyte counts, ADA levels, IgA, IgG and IgM titers, percentage of CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), CD16(+)CD56(+), CD3(−)CD19(+), and CD19(+)CD23(+) lymphocytes, and CD4(+)/CD8(+) ratio between EBV-related disease groups were all statistically significant (P < 0.01). ADA levels in the EBV-related disease groups were significantly higher than those in the control group (P < 0.01). The lymphocyte count, ADA levels, IgA and IgG titers, and percentage of CD3(+) and CD3(+)CD8 + lymphocytes in the atypical EBV infection, EBV-IM1, and EBV-IM2 groups were significantly higher than those in the EBV-RTI, AUTI, and control groups (P < 0.01), whereas the percentage of CD3(+)CD4(+), CD3(−)CD19(+), and CD19(+)CD23(+) lymphocytes and CD4(+)/CD8(+) ratio showed the opposite trend. ADA levels were consistent with and closely related to the viral load and cellular and humoral immunity in EBV-related diseases. CONCLUSIONS: ADA levels, humoral immunity, and cellular immunity were diverse in EBV-related diseases, and ADA was closely related to Igs and lymphocyte subsets. BioMed Central 2023-04-24 /pmc/articles/PMC10127006/ /pubmed/37095577 http://dx.doi.org/10.1186/s13052-023-01457-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Shi, Ting Ding, Qi Liu, Xinglou Ai, Guo Zhou, Hua Huang, Linlin Concordance of adenosine deaminase with immunoglobulins and lymphocyte subsets in EBV-related diseases |
title | Concordance of adenosine deaminase with immunoglobulins and lymphocyte subsets in EBV-related diseases |
title_full | Concordance of adenosine deaminase with immunoglobulins and lymphocyte subsets in EBV-related diseases |
title_fullStr | Concordance of adenosine deaminase with immunoglobulins and lymphocyte subsets in EBV-related diseases |
title_full_unstemmed | Concordance of adenosine deaminase with immunoglobulins and lymphocyte subsets in EBV-related diseases |
title_short | Concordance of adenosine deaminase with immunoglobulins and lymphocyte subsets in EBV-related diseases |
title_sort | concordance of adenosine deaminase with immunoglobulins and lymphocyte subsets in ebv-related diseases |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127006/ https://www.ncbi.nlm.nih.gov/pubmed/37095577 http://dx.doi.org/10.1186/s13052-023-01457-0 |
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