A Quick DNA Extraction Method for High Throughput Screening in Gram-positive Bacteria

In this study, a sonication-based DNA extraction method was developed, in which the whole process can be finished within 10 min. This method is almost zero cost and time-saving, which is useful for high throughput screening, especially in the screening of mutants generated in random mutagenesis. Thi...

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Detalles Bibliográficos
Autores principales: Chen, Nuo, Yuan, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127043/
https://www.ncbi.nlm.nih.gov/pubmed/37113329
http://dx.doi.org/10.21769/BioProtoc.4653
Descripción
Sumario:In this study, a sonication-based DNA extraction method was developed, in which the whole process can be finished within 10 min. This method is almost zero cost and time-saving, which is useful for high throughput screening, especially in the screening of mutants generated in random mutagenesis. This method is effective in genomic DNA extraction for PCR amplification in several Gram-positive bacteria, including Bacillus cereus, Bacillus thuringiensis, Bacillus subtilis, and Listeria monocytogenes.

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