A Quick DNA Extraction Method for High Throughput Screening in Gram-positive Bacteria

In this study, a sonication-based DNA extraction method was developed, in which the whole process can be finished within 10 min. This method is almost zero cost and time-saving, which is useful for high throughput screening, especially in the screening of mutants generated in random mutagenesis. Thi...

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Detalles Bibliográficos
Autores principales: Chen, Nuo, Yuan, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127043/
https://www.ncbi.nlm.nih.gov/pubmed/37113329
http://dx.doi.org/10.21769/BioProtoc.4653
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author Chen, Nuo
Yuan, Xiaoming
author_facet Chen, Nuo
Yuan, Xiaoming
author_sort Chen, Nuo
collection PubMed
description In this study, a sonication-based DNA extraction method was developed, in which the whole process can be finished within 10 min. This method is almost zero cost and time-saving, which is useful for high throughput screening, especially in the screening of mutants generated in random mutagenesis. This method is effective in genomic DNA extraction for PCR amplification in several Gram-positive bacteria, including Bacillus cereus, Bacillus thuringiensis, Bacillus subtilis, and Listeria monocytogenes.
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spelling pubmed-101270432023-04-26 A Quick DNA Extraction Method for High Throughput Screening in Gram-positive Bacteria Chen, Nuo Yuan, Xiaoming Bio Protoc Methods Article In this study, a sonication-based DNA extraction method was developed, in which the whole process can be finished within 10 min. This method is almost zero cost and time-saving, which is useful for high throughput screening, especially in the screening of mutants generated in random mutagenesis. This method is effective in genomic DNA extraction for PCR amplification in several Gram-positive bacteria, including Bacillus cereus, Bacillus thuringiensis, Bacillus subtilis, and Listeria monocytogenes. Bio-Protocol 2023-04-20 /pmc/articles/PMC10127043/ /pubmed/37113329 http://dx.doi.org/10.21769/BioProtoc.4653 Text en Copyright © 2023 The Authors; https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Methods Article
Chen, Nuo
Yuan, Xiaoming
A Quick DNA Extraction Method for High Throughput Screening in Gram-positive Bacteria
title A Quick DNA Extraction Method for High Throughput Screening in Gram-positive Bacteria
title_full A Quick DNA Extraction Method for High Throughput Screening in Gram-positive Bacteria
title_fullStr A Quick DNA Extraction Method for High Throughput Screening in Gram-positive Bacteria
title_full_unstemmed A Quick DNA Extraction Method for High Throughput Screening in Gram-positive Bacteria
title_short A Quick DNA Extraction Method for High Throughput Screening in Gram-positive Bacteria
title_sort quick dna extraction method for high throughput screening in gram-positive bacteria
topic Methods Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127043/
https://www.ncbi.nlm.nih.gov/pubmed/37113329
http://dx.doi.org/10.21769/BioProtoc.4653
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