Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles

[Image: see text] In cell-free gene expression, low input DNA concentration severely limits the phenotypic output, which may impair in vitro protein evolution efforts. We address this challenge by developing CADGE, a strategy that is based on clonal isothermal amplification of a linear gene-encoding...

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Detalles Bibliográficos
Autores principales: Abil, Zhanar, Restrepo Sierra, Ana Maria, Danelon, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127449/
https://www.ncbi.nlm.nih.gov/pubmed/37014369
http://dx.doi.org/10.1021/acssynbio.2c00668

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127449/
https://www.ncbi.nlm.nih.gov/pubmed/37014369
http://dx.doi.org/10.1021/acssynbio.2c00668

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