Cargando…
Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles
[Image: see text] In cell-free gene expression, low input DNA concentration severely limits the phenotypic output, which may impair in vitro protein evolution efforts. We address this challenge by developing CADGE, a strategy that is based on clonal isothermal amplification of a linear gene-encoding...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2023
|
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127449/ https://www.ncbi.nlm.nih.gov/pubmed/37014369 http://dx.doi.org/10.1021/acssynbio.2c00668 |