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FliL Functions in Diverse Microbes to Negatively Modulate Motor Output via Its N-Terminal Region
The flagellar motor protein FliL is conserved across many microbes, but its exact role has been obscured by varying fliL mutant phenotypes. We reanalyzed results from fliL studies and found they utilized alleles that differed in the amount of N- and C-terminal regions that were retained. Alleles tha...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127578/ https://www.ncbi.nlm.nih.gov/pubmed/36852985 http://dx.doi.org/10.1128/mbio.00283-23 |
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author | Liu, Xiaolin Roujeinikova, Anna Ottemann, Karen M. |
author_facet | Liu, Xiaolin Roujeinikova, Anna Ottemann, Karen M. |
author_sort | Liu, Xiaolin |
collection | PubMed |
description | The flagellar motor protein FliL is conserved across many microbes, but its exact role has been obscured by varying fliL mutant phenotypes. We reanalyzed results from fliL studies and found they utilized alleles that differed in the amount of N- and C-terminal regions that were retained. Alleles that retain the N-terminal cytoplasmic and transmembrane helix (TM) regions in the absence of the C-terminal periplasmic domain result in loss of motility, while alleles that completely lack the N-terminal region, independent of the periplasmic domain, retain motility. We then tested this prediction in Helicobacter pylori fliL and found support for the idea. This analysis suggests that FliL function may be more conserved across bacteria than previously thought, that it is not essential for motility, and that the N-terminal region has the negative ability to regulate motor function. |
format | Online Article Text |
id | pubmed-10127578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-101275782023-04-26 FliL Functions in Diverse Microbes to Negatively Modulate Motor Output via Its N-Terminal Region Liu, Xiaolin Roujeinikova, Anna Ottemann, Karen M. mBio Observation The flagellar motor protein FliL is conserved across many microbes, but its exact role has been obscured by varying fliL mutant phenotypes. We reanalyzed results from fliL studies and found they utilized alleles that differed in the amount of N- and C-terminal regions that were retained. Alleles that retain the N-terminal cytoplasmic and transmembrane helix (TM) regions in the absence of the C-terminal periplasmic domain result in loss of motility, while alleles that completely lack the N-terminal region, independent of the periplasmic domain, retain motility. We then tested this prediction in Helicobacter pylori fliL and found support for the idea. This analysis suggests that FliL function may be more conserved across bacteria than previously thought, that it is not essential for motility, and that the N-terminal region has the negative ability to regulate motor function. American Society for Microbiology 2023-02-28 /pmc/articles/PMC10127578/ /pubmed/36852985 http://dx.doi.org/10.1128/mbio.00283-23 Text en Copyright © 2023 Liu et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Observation Liu, Xiaolin Roujeinikova, Anna Ottemann, Karen M. FliL Functions in Diverse Microbes to Negatively Modulate Motor Output via Its N-Terminal Region |
title | FliL Functions in Diverse Microbes to Negatively Modulate Motor Output via Its N-Terminal Region |
title_full | FliL Functions in Diverse Microbes to Negatively Modulate Motor Output via Its N-Terminal Region |
title_fullStr | FliL Functions in Diverse Microbes to Negatively Modulate Motor Output via Its N-Terminal Region |
title_full_unstemmed | FliL Functions in Diverse Microbes to Negatively Modulate Motor Output via Its N-Terminal Region |
title_short | FliL Functions in Diverse Microbes to Negatively Modulate Motor Output via Its N-Terminal Region |
title_sort | flil functions in diverse microbes to negatively modulate motor output via its n-terminal region |
topic | Observation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127578/ https://www.ncbi.nlm.nih.gov/pubmed/36852985 http://dx.doi.org/10.1128/mbio.00283-23 |
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