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Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes

Developing effective tools to control mosquito populations is essential for reducing the incidence of diseases like malaria and dengue. Biopesticides of microbial origin are a rich, underexplored source of mosquitocidal compounds. We previously developed a biopesticide from the bacterium Chromobacte...

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Autores principales: Engdahl, Cecilia Springer, Caragata, Eric P., Tavadia, Mihra, Dimopoulos, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127667/
https://www.ncbi.nlm.nih.gov/pubmed/37017525
http://dx.doi.org/10.1128/mbio.00480-23
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author Engdahl, Cecilia Springer
Caragata, Eric P.
Tavadia, Mihra
Dimopoulos, George
author_facet Engdahl, Cecilia Springer
Caragata, Eric P.
Tavadia, Mihra
Dimopoulos, George
author_sort Engdahl, Cecilia Springer
collection PubMed
description Developing effective tools to control mosquito populations is essential for reducing the incidence of diseases like malaria and dengue. Biopesticides of microbial origin are a rich, underexplored source of mosquitocidal compounds. We previously developed a biopesticide from the bacterium Chromobacterium sp. Panama that rapidly kills vector mosquito larvae, including Aedes aegypti and Anopheles gambiae. Here, we demonstrate that two independent Ae. aegypti colonies exposed to a sublethal dose of that biopesticide over consecutive generations persistently exhibited high mortality and developmental delays, indicating that resistance did not develop during the study period. Critically, the descendants of biopesticide-exposed mosquitoes experienced decreased longevity and did not display increased susceptibility to dengue virus or decreased susceptibility to common chemical insecticides. Through RNA sequencing, we observed no link between biopesticide exposure and the increased activity of xenobiotic metabolism and detoxification genes typically associated with insecticide resistance. These findings indicate that the Chromobacterium biopesticide is an exciting, emerging mosquito control tool.
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spelling pubmed-101276672023-04-26 Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes Engdahl, Cecilia Springer Caragata, Eric P. Tavadia, Mihra Dimopoulos, George mBio Research Article Developing effective tools to control mosquito populations is essential for reducing the incidence of diseases like malaria and dengue. Biopesticides of microbial origin are a rich, underexplored source of mosquitocidal compounds. We previously developed a biopesticide from the bacterium Chromobacterium sp. Panama that rapidly kills vector mosquito larvae, including Aedes aegypti and Anopheles gambiae. Here, we demonstrate that two independent Ae. aegypti colonies exposed to a sublethal dose of that biopesticide over consecutive generations persistently exhibited high mortality and developmental delays, indicating that resistance did not develop during the study period. Critically, the descendants of biopesticide-exposed mosquitoes experienced decreased longevity and did not display increased susceptibility to dengue virus or decreased susceptibility to common chemical insecticides. Through RNA sequencing, we observed no link between biopesticide exposure and the increased activity of xenobiotic metabolism and detoxification genes typically associated with insecticide resistance. These findings indicate that the Chromobacterium biopesticide is an exciting, emerging mosquito control tool. American Society for Microbiology 2023-04-05 /pmc/articles/PMC10127667/ /pubmed/37017525 http://dx.doi.org/10.1128/mbio.00480-23 Text en Copyright © 2023 Engdahl et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Engdahl, Cecilia Springer
Caragata, Eric P.
Tavadia, Mihra
Dimopoulos, George
Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes
title Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes
title_full Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes
title_fullStr Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes
title_full_unstemmed Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes
title_short Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes
title_sort chromobacterium biopesticide exposure does not select for resistance in aedes mosquitoes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127667/
https://www.ncbi.nlm.nih.gov/pubmed/37017525
http://dx.doi.org/10.1128/mbio.00480-23
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