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Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes
Developing effective tools to control mosquito populations is essential for reducing the incidence of diseases like malaria and dengue. Biopesticides of microbial origin are a rich, underexplored source of mosquitocidal compounds. We previously developed a biopesticide from the bacterium Chromobacte...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127667/ https://www.ncbi.nlm.nih.gov/pubmed/37017525 http://dx.doi.org/10.1128/mbio.00480-23 |
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author | Engdahl, Cecilia Springer Caragata, Eric P. Tavadia, Mihra Dimopoulos, George |
author_facet | Engdahl, Cecilia Springer Caragata, Eric P. Tavadia, Mihra Dimopoulos, George |
author_sort | Engdahl, Cecilia Springer |
collection | PubMed |
description | Developing effective tools to control mosquito populations is essential for reducing the incidence of diseases like malaria and dengue. Biopesticides of microbial origin are a rich, underexplored source of mosquitocidal compounds. We previously developed a biopesticide from the bacterium Chromobacterium sp. Panama that rapidly kills vector mosquito larvae, including Aedes aegypti and Anopheles gambiae. Here, we demonstrate that two independent Ae. aegypti colonies exposed to a sublethal dose of that biopesticide over consecutive generations persistently exhibited high mortality and developmental delays, indicating that resistance did not develop during the study period. Critically, the descendants of biopesticide-exposed mosquitoes experienced decreased longevity and did not display increased susceptibility to dengue virus or decreased susceptibility to common chemical insecticides. Through RNA sequencing, we observed no link between biopesticide exposure and the increased activity of xenobiotic metabolism and detoxification genes typically associated with insecticide resistance. These findings indicate that the Chromobacterium biopesticide is an exciting, emerging mosquito control tool. |
format | Online Article Text |
id | pubmed-10127667 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-101276672023-04-26 Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes Engdahl, Cecilia Springer Caragata, Eric P. Tavadia, Mihra Dimopoulos, George mBio Research Article Developing effective tools to control mosquito populations is essential for reducing the incidence of diseases like malaria and dengue. Biopesticides of microbial origin are a rich, underexplored source of mosquitocidal compounds. We previously developed a biopesticide from the bacterium Chromobacterium sp. Panama that rapidly kills vector mosquito larvae, including Aedes aegypti and Anopheles gambiae. Here, we demonstrate that two independent Ae. aegypti colonies exposed to a sublethal dose of that biopesticide over consecutive generations persistently exhibited high mortality and developmental delays, indicating that resistance did not develop during the study period. Critically, the descendants of biopesticide-exposed mosquitoes experienced decreased longevity and did not display increased susceptibility to dengue virus or decreased susceptibility to common chemical insecticides. Through RNA sequencing, we observed no link between biopesticide exposure and the increased activity of xenobiotic metabolism and detoxification genes typically associated with insecticide resistance. These findings indicate that the Chromobacterium biopesticide is an exciting, emerging mosquito control tool. American Society for Microbiology 2023-04-05 /pmc/articles/PMC10127667/ /pubmed/37017525 http://dx.doi.org/10.1128/mbio.00480-23 Text en Copyright © 2023 Engdahl et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Engdahl, Cecilia Springer Caragata, Eric P. Tavadia, Mihra Dimopoulos, George Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes |
title | Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes |
title_full | Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes |
title_fullStr | Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes |
title_full_unstemmed | Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes |
title_short | Chromobacterium Biopesticide Exposure Does Not Select for Resistance in Aedes Mosquitoes |
title_sort | chromobacterium biopesticide exposure does not select for resistance in aedes mosquitoes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127667/ https://www.ncbi.nlm.nih.gov/pubmed/37017525 http://dx.doi.org/10.1128/mbio.00480-23 |
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