Fundamental insights in early‐stage inclusion body formation

Early‐stage inclusion body formation is still mysterious. Literature is ambiguous about the existence of rod‐shaped protein aggregates, a potential sponge‐like inclusion body scaffold as well as the number of inclusion bodies per Escherichia coli cell. In this study, we verified the existence of rod...

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Detalles Bibliográficos
Autores principales: Kopp, Julian, Bayer, Benjamin, Slouka, Christoph, Striedner, Gerald, Dürkop, Mark, Spadiut, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10128139/
https://www.ncbi.nlm.nih.gov/pubmed/35830603
http://dx.doi.org/10.1111/1751-7915.14117
Descripción
Sumario:Early‐stage inclusion body formation is still mysterious. Literature is ambiguous about the existence of rod‐shaped protein aggregates, a potential sponge‐like inclusion body scaffold as well as the number of inclusion bodies per Escherichia coli cell. In this study, we verified the existence of rod‐shaped inclusion bodies, confirmed their porous morphology, the presence of multiple protein aggregates per cell and modelled inclusion body formation as function of the number of generations.

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