Fundamental insights in early‐stage inclusion body formation

Early‐stage inclusion body formation is still mysterious. Literature is ambiguous about the existence of rod‐shaped protein aggregates, a potential sponge‐like inclusion body scaffold as well as the number of inclusion bodies per Escherichia coli cell. In this study, we verified the existence of rod...

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Autores principales: Kopp, Julian, Bayer, Benjamin, Slouka, Christoph, Striedner, Gerald, Dürkop, Mark, Spadiut, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10128139/
https://www.ncbi.nlm.nih.gov/pubmed/35830603
http://dx.doi.org/10.1111/1751-7915.14117
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author Kopp, Julian
Bayer, Benjamin
Slouka, Christoph
Striedner, Gerald
Dürkop, Mark
Spadiut, Oliver
author_facet Kopp, Julian
Bayer, Benjamin
Slouka, Christoph
Striedner, Gerald
Dürkop, Mark
Spadiut, Oliver
author_sort Kopp, Julian
collection PubMed
description Early‐stage inclusion body formation is still mysterious. Literature is ambiguous about the existence of rod‐shaped protein aggregates, a potential sponge‐like inclusion body scaffold as well as the number of inclusion bodies per Escherichia coli cell. In this study, we verified the existence of rod‐shaped inclusion bodies, confirmed their porous morphology, the presence of multiple protein aggregates per cell and modelled inclusion body formation as function of the number of generations.
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spelling pubmed-101281392023-04-26 Fundamental insights in early‐stage inclusion body formation Kopp, Julian Bayer, Benjamin Slouka, Christoph Striedner, Gerald Dürkop, Mark Spadiut, Oliver Microb Biotechnol Article Early‐stage inclusion body formation is still mysterious. Literature is ambiguous about the existence of rod‐shaped protein aggregates, a potential sponge‐like inclusion body scaffold as well as the number of inclusion bodies per Escherichia coli cell. In this study, we verified the existence of rod‐shaped inclusion bodies, confirmed their porous morphology, the presence of multiple protein aggregates per cell and modelled inclusion body formation as function of the number of generations. John Wiley and Sons Inc. 2022-07-13 /pmc/articles/PMC10128139/ /pubmed/35830603 http://dx.doi.org/10.1111/1751-7915.14117 Text en © 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Kopp, Julian
Bayer, Benjamin
Slouka, Christoph
Striedner, Gerald
Dürkop, Mark
Spadiut, Oliver
Fundamental insights in early‐stage inclusion body formation
title Fundamental insights in early‐stage inclusion body formation
title_full Fundamental insights in early‐stage inclusion body formation
title_fullStr Fundamental insights in early‐stage inclusion body formation
title_full_unstemmed Fundamental insights in early‐stage inclusion body formation
title_short Fundamental insights in early‐stage inclusion body formation
title_sort fundamental insights in early‐stage inclusion body formation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10128139/
https://www.ncbi.nlm.nih.gov/pubmed/35830603
http://dx.doi.org/10.1111/1751-7915.14117
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