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Genomic capacities for Reactive Oxygen Species metabolism across marine phytoplankton
Marine phytoplankton produce and scavenge Reactive Oxygen Species, to support cellular processes, while limiting damaging reactions. Some prokaryotic picophytoplankton have, however, lost all genes encoding scavenging of hydrogen peroxide. Such losses of metabolic function can only apply to Reactive...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10128935/ https://www.ncbi.nlm.nih.gov/pubmed/37098087 http://dx.doi.org/10.1371/journal.pone.0284580 |
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author | Omar, Naaman M. Fleury, Katherine Beardsall, Brian Prášil, Ondřej Campbell, Douglas A. |
author_facet | Omar, Naaman M. Fleury, Katherine Beardsall, Brian Prášil, Ondřej Campbell, Douglas A. |
author_sort | Omar, Naaman M. |
collection | PubMed |
description | Marine phytoplankton produce and scavenge Reactive Oxygen Species, to support cellular processes, while limiting damaging reactions. Some prokaryotic picophytoplankton have, however, lost all genes encoding scavenging of hydrogen peroxide. Such losses of metabolic function can only apply to Reactive Oxygen Species which potentially traverse the cell membrane outwards, before provoking damaging intracellular reactions. We hypothesized that cell radius influences which elements of Reactive Oxygen Species metabolism are partially or fully dispensable from a cell. We therefore investigated genomes and transcriptomes from diverse marine eukaryotic phytoplankton, ranging from 0.4 to 44 μm radius, to analyze the genomic allocations encoding enzymes metabolizing Reactive Oxygen Species. Superoxide has high reactivity, short lifetimes and limited membrane permeability. Genes encoding superoxide scavenging are ubiquitous across phytoplankton, but the fractional gene allocation decreased with increasing cell radius, consistent with a nearly fixed set of core genes for scavenging superoxide pools. Hydrogen peroxide has lower reactivity, longer intracellular and extracellular lifetimes and readily crosses cell membranes. Genomic allocations to both hydrogen peroxide production and scavenging decrease with increasing cell radius. Nitric Oxide has low reactivity, long intracellular and extracellular lifetimes and readily crosses cell membranes. Neither Nitric Oxide production nor scavenging genomic allocations changed with increasing cell radius. Many taxa, however, lack the genomic capacity for nitric oxide production or scavenging. The probability of presence of capacity to produce nitric oxide decreases with increasing cell size, and is influenced by flagella and colony formation. In contrast, the probability of presence of capacity to scavenge nitric oxide increases with increasing cell size, and is again influenced by flagella and colony formation. |
format | Online Article Text |
id | pubmed-10128935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-101289352023-04-26 Genomic capacities for Reactive Oxygen Species metabolism across marine phytoplankton Omar, Naaman M. Fleury, Katherine Beardsall, Brian Prášil, Ondřej Campbell, Douglas A. PLoS One Research Article Marine phytoplankton produce and scavenge Reactive Oxygen Species, to support cellular processes, while limiting damaging reactions. Some prokaryotic picophytoplankton have, however, lost all genes encoding scavenging of hydrogen peroxide. Such losses of metabolic function can only apply to Reactive Oxygen Species which potentially traverse the cell membrane outwards, before provoking damaging intracellular reactions. We hypothesized that cell radius influences which elements of Reactive Oxygen Species metabolism are partially or fully dispensable from a cell. We therefore investigated genomes and transcriptomes from diverse marine eukaryotic phytoplankton, ranging from 0.4 to 44 μm radius, to analyze the genomic allocations encoding enzymes metabolizing Reactive Oxygen Species. Superoxide has high reactivity, short lifetimes and limited membrane permeability. Genes encoding superoxide scavenging are ubiquitous across phytoplankton, but the fractional gene allocation decreased with increasing cell radius, consistent with a nearly fixed set of core genes for scavenging superoxide pools. Hydrogen peroxide has lower reactivity, longer intracellular and extracellular lifetimes and readily crosses cell membranes. Genomic allocations to both hydrogen peroxide production and scavenging decrease with increasing cell radius. Nitric Oxide has low reactivity, long intracellular and extracellular lifetimes and readily crosses cell membranes. Neither Nitric Oxide production nor scavenging genomic allocations changed with increasing cell radius. Many taxa, however, lack the genomic capacity for nitric oxide production or scavenging. The probability of presence of capacity to produce nitric oxide decreases with increasing cell size, and is influenced by flagella and colony formation. In contrast, the probability of presence of capacity to scavenge nitric oxide increases with increasing cell size, and is again influenced by flagella and colony formation. Public Library of Science 2023-04-25 /pmc/articles/PMC10128935/ /pubmed/37098087 http://dx.doi.org/10.1371/journal.pone.0284580 Text en © 2023 Omar et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Omar, Naaman M. Fleury, Katherine Beardsall, Brian Prášil, Ondřej Campbell, Douglas A. Genomic capacities for Reactive Oxygen Species metabolism across marine phytoplankton |
title | Genomic capacities for Reactive Oxygen Species metabolism across marine phytoplankton |
title_full | Genomic capacities for Reactive Oxygen Species metabolism across marine phytoplankton |
title_fullStr | Genomic capacities for Reactive Oxygen Species metabolism across marine phytoplankton |
title_full_unstemmed | Genomic capacities for Reactive Oxygen Species metabolism across marine phytoplankton |
title_short | Genomic capacities for Reactive Oxygen Species metabolism across marine phytoplankton |
title_sort | genomic capacities for reactive oxygen species metabolism across marine phytoplankton |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10128935/ https://www.ncbi.nlm.nih.gov/pubmed/37098087 http://dx.doi.org/10.1371/journal.pone.0284580 |
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