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Protective effects of calorie restriction and 17-β estradiol on cardiac hypertrophy in ovariectomized obese rats

Obesity and menopause lead to cardiovascular diseases. Calorie restriction (CR) can modulate estrogen deficiency and obesity-related cardiovascular diseases. The protective effects of CR and estradiol on cardiac hypertrophy in ovariectomized obese rats were explored in this study. The adult female W...

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Autores principales: Hajializadeh, Zahra, Khaksari, Mohammad, Dabiri, Shahriar, Darvishzadeh Mahani, Fatemeh, Raji-Amirhasani, Alireza, Bejeshk, Mohammad Abbas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10128952/
https://www.ncbi.nlm.nih.gov/pubmed/37098007
http://dx.doi.org/10.1371/journal.pone.0282089
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author Hajializadeh, Zahra
Khaksari, Mohammad
Dabiri, Shahriar
Darvishzadeh Mahani, Fatemeh
Raji-Amirhasani, Alireza
Bejeshk, Mohammad Abbas
author_facet Hajializadeh, Zahra
Khaksari, Mohammad
Dabiri, Shahriar
Darvishzadeh Mahani, Fatemeh
Raji-Amirhasani, Alireza
Bejeshk, Mohammad Abbas
author_sort Hajializadeh, Zahra
collection PubMed
description Obesity and menopause lead to cardiovascular diseases. Calorie restriction (CR) can modulate estrogen deficiency and obesity-related cardiovascular diseases. The protective effects of CR and estradiol on cardiac hypertrophy in ovariectomized obese rats were explored in this study. The adult female Wistar rats were divided into sham and ovariectomized (OVX) groups that received a high-fat diet (60% HFD) or standard diet (SD) or 30% CR for 16 weeks, and then, 1mg/kg E2 (17-β estradiol) was injected intraperitoneally every 4 days for four weeks in OVX-rats. Hemodynamic parameters were evaluated before and after each diet. Heart tissues were collected for biochemical, histological, and molecular analysis. HFD consumption led to weight gain in sham and OVX rats. In contrast, CR and E2 led to body weight loss in these animals. Also, heart weight (HW), heart weight/body weight (HW/BW) ratio, and left ventricular weight (LVW) were enhanced in OVX rats that received SD and HFD. E2 reduced these indexes in both diet conditions but reduction effects of CR were seen only in HFD groups. HFD and SD feeding increased hemodynamic parameters, ANP (atrial natriuretic peptide) mRNA expression, and TGF-β1(transforming growth factor-beta 1) protein level in the OVX animals, while CR and E2 reduced these factors. Cardiomyocyte diameter and hydroxyproline content were increased in the OVX-HFD groups. Nevertheless, CR and E2 decreased these indicators. The results showed that CR and E2 treatment reduced obesity-induced-cardiac hypertrophy in ovariectomized groups (20% and 24% respectively). CR appears to have almost as reducing effects as estrogen therapy on cardiac hypertrophy. The findings suggest that CR can be considered a therapeutic candidate for postmenopausal cardiovascular disease.
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spelling pubmed-101289522023-04-26 Protective effects of calorie restriction and 17-β estradiol on cardiac hypertrophy in ovariectomized obese rats Hajializadeh, Zahra Khaksari, Mohammad Dabiri, Shahriar Darvishzadeh Mahani, Fatemeh Raji-Amirhasani, Alireza Bejeshk, Mohammad Abbas PLoS One Research Article Obesity and menopause lead to cardiovascular diseases. Calorie restriction (CR) can modulate estrogen deficiency and obesity-related cardiovascular diseases. The protective effects of CR and estradiol on cardiac hypertrophy in ovariectomized obese rats were explored in this study. The adult female Wistar rats were divided into sham and ovariectomized (OVX) groups that received a high-fat diet (60% HFD) or standard diet (SD) or 30% CR for 16 weeks, and then, 1mg/kg E2 (17-β estradiol) was injected intraperitoneally every 4 days for four weeks in OVX-rats. Hemodynamic parameters were evaluated before and after each diet. Heart tissues were collected for biochemical, histological, and molecular analysis. HFD consumption led to weight gain in sham and OVX rats. In contrast, CR and E2 led to body weight loss in these animals. Also, heart weight (HW), heart weight/body weight (HW/BW) ratio, and left ventricular weight (LVW) were enhanced in OVX rats that received SD and HFD. E2 reduced these indexes in both diet conditions but reduction effects of CR were seen only in HFD groups. HFD and SD feeding increased hemodynamic parameters, ANP (atrial natriuretic peptide) mRNA expression, and TGF-β1(transforming growth factor-beta 1) protein level in the OVX animals, while CR and E2 reduced these factors. Cardiomyocyte diameter and hydroxyproline content were increased in the OVX-HFD groups. Nevertheless, CR and E2 decreased these indicators. The results showed that CR and E2 treatment reduced obesity-induced-cardiac hypertrophy in ovariectomized groups (20% and 24% respectively). CR appears to have almost as reducing effects as estrogen therapy on cardiac hypertrophy. The findings suggest that CR can be considered a therapeutic candidate for postmenopausal cardiovascular disease. Public Library of Science 2023-04-25 /pmc/articles/PMC10128952/ /pubmed/37098007 http://dx.doi.org/10.1371/journal.pone.0282089 Text en © 2023 Hajializadeh et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hajializadeh, Zahra
Khaksari, Mohammad
Dabiri, Shahriar
Darvishzadeh Mahani, Fatemeh
Raji-Amirhasani, Alireza
Bejeshk, Mohammad Abbas
Protective effects of calorie restriction and 17-β estradiol on cardiac hypertrophy in ovariectomized obese rats
title Protective effects of calorie restriction and 17-β estradiol on cardiac hypertrophy in ovariectomized obese rats
title_full Protective effects of calorie restriction and 17-β estradiol on cardiac hypertrophy in ovariectomized obese rats
title_fullStr Protective effects of calorie restriction and 17-β estradiol on cardiac hypertrophy in ovariectomized obese rats
title_full_unstemmed Protective effects of calorie restriction and 17-β estradiol on cardiac hypertrophy in ovariectomized obese rats
title_short Protective effects of calorie restriction and 17-β estradiol on cardiac hypertrophy in ovariectomized obese rats
title_sort protective effects of calorie restriction and 17-β estradiol on cardiac hypertrophy in ovariectomized obese rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10128952/
https://www.ncbi.nlm.nih.gov/pubmed/37098007
http://dx.doi.org/10.1371/journal.pone.0282089
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