Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes

CRISPR-mediated genome editing of primary human lymphocytes is typically carried out via electroporation, which can be cytotoxic, cumbersome and costly. Here we show that the yields of edited primary human lymphocytes can be increased substantially by delivering a CRISPR ribonucleoprotein mixed with...

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Detalles Bibliográficos
Autores principales: Foss, Dana V., Muldoon, Joseph J., Nguyen, David N., Carr, Daniel, Sahu, Srishti U., Hunsinger, John M., Wyman, Stacia K., Krishnappa, Netravathi, Mendonsa, Rima, Schanzer, Elaine V., Shy, Brian R., Vykunta, Vivasvan S., Allain, Vincent, Li, Zhongmei, Marson, Alexander, Eyquem, Justin, Wilson, Ross C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10129304/
https://www.ncbi.nlm.nih.gov/pubmed/37147433
http://dx.doi.org/10.1038/s41551-023-01032-2

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