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Meclofenamate Suppresses MUC5AC Mucin Gene Expression by Regulating the NF-kB Signaling Pathway in Human Pulmonary Mucoepidermoid NCI-H292 Cells

The current study aimed to reveal the potential effect of meclofenamate, a nonsteroidal anti-inflammatory drug, on the gene expression of airway MUC5AC mucin. Human pulmonary mucoepidermoid NCI-H292 cells were pretreated with meclofenamate for 30 min and stimulated with phorbol 12-myristate 13-aceta...

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Autores principales: Ryu, Jiho, Kim, Kyung-il, Hossain, Rajib, Lee, Misoon, Hong, Jin Tae, Lee, Hyun Jae, Lee, Choong Jae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Applied Pharmacology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10129850/
https://www.ncbi.nlm.nih.gov/pubmed/37024262
http://dx.doi.org/10.4062/biomolther.2023.035
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author Ryu, Jiho
Kim, Kyung-il
Hossain, Rajib
Lee, Misoon
Hong, Jin Tae
Lee, Hyun Jae
Lee, Choong Jae
author_facet Ryu, Jiho
Kim, Kyung-il
Hossain, Rajib
Lee, Misoon
Hong, Jin Tae
Lee, Hyun Jae
Lee, Choong Jae
author_sort Ryu, Jiho
collection PubMed
description The current study aimed to reveal the potential effect of meclofenamate, a nonsteroidal anti-inflammatory drug, on the gene expression of airway MUC5AC mucin. Human pulmonary mucoepidermoid NCI-H292 cells were pretreated with meclofenamate for 30 min and stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 h. Thereafter, the effect of meclofenamate on the PMA-induced nuclear factor kappa B (NF-kB) signaling pathway was assessed. Meclofenamate inhibited glycoprotein production and mRNA expression of MUC5AC mucins induced by PMA by inhibiting the degradation of inhibitory kappa Bα (IkBα) and NF-kB p65 nuclear translocation. These results suggest meclofenamate suppresses mucin gene expression by regulating NF-kB signaling pathway in human pulmonary epithelial cells.
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spelling pubmed-101298502023-04-27 Meclofenamate Suppresses MUC5AC Mucin Gene Expression by Regulating the NF-kB Signaling Pathway in Human Pulmonary Mucoepidermoid NCI-H292 Cells Ryu, Jiho Kim, Kyung-il Hossain, Rajib Lee, Misoon Hong, Jin Tae Lee, Hyun Jae Lee, Choong Jae Biomol Ther (Seoul) Original Article The current study aimed to reveal the potential effect of meclofenamate, a nonsteroidal anti-inflammatory drug, on the gene expression of airway MUC5AC mucin. Human pulmonary mucoepidermoid NCI-H292 cells were pretreated with meclofenamate for 30 min and stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 h. Thereafter, the effect of meclofenamate on the PMA-induced nuclear factor kappa B (NF-kB) signaling pathway was assessed. Meclofenamate inhibited glycoprotein production and mRNA expression of MUC5AC mucins induced by PMA by inhibiting the degradation of inhibitory kappa Bα (IkBα) and NF-kB p65 nuclear translocation. These results suggest meclofenamate suppresses mucin gene expression by regulating NF-kB signaling pathway in human pulmonary epithelial cells. The Korean Society of Applied Pharmacology 2023-05-01 2023-04-07 /pmc/articles/PMC10129850/ /pubmed/37024262 http://dx.doi.org/10.4062/biomolther.2023.035 Text en Copyright © 2023, The Korean Society of Applied Pharmacology https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ryu, Jiho
Kim, Kyung-il
Hossain, Rajib
Lee, Misoon
Hong, Jin Tae
Lee, Hyun Jae
Lee, Choong Jae
Meclofenamate Suppresses MUC5AC Mucin Gene Expression by Regulating the NF-kB Signaling Pathway in Human Pulmonary Mucoepidermoid NCI-H292 Cells
title Meclofenamate Suppresses MUC5AC Mucin Gene Expression by Regulating the NF-kB Signaling Pathway in Human Pulmonary Mucoepidermoid NCI-H292 Cells
title_full Meclofenamate Suppresses MUC5AC Mucin Gene Expression by Regulating the NF-kB Signaling Pathway in Human Pulmonary Mucoepidermoid NCI-H292 Cells
title_fullStr Meclofenamate Suppresses MUC5AC Mucin Gene Expression by Regulating the NF-kB Signaling Pathway in Human Pulmonary Mucoepidermoid NCI-H292 Cells
title_full_unstemmed Meclofenamate Suppresses MUC5AC Mucin Gene Expression by Regulating the NF-kB Signaling Pathway in Human Pulmonary Mucoepidermoid NCI-H292 Cells
title_short Meclofenamate Suppresses MUC5AC Mucin Gene Expression by Regulating the NF-kB Signaling Pathway in Human Pulmonary Mucoepidermoid NCI-H292 Cells
title_sort meclofenamate suppresses muc5ac mucin gene expression by regulating the nf-kb signaling pathway in human pulmonary mucoepidermoid nci-h292 cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10129850/
https://www.ncbi.nlm.nih.gov/pubmed/37024262
http://dx.doi.org/10.4062/biomolther.2023.035
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