Millisecond cryo-trapping by the spitrobot crystal plunger simplifies time-resolved crystallography

We introduce the spitrobot, a protein crystal plunger, enabling reaction quenching via cryo-trapping with a time-resolution in the millisecond range. Protein crystals are mounted on canonical micromeshes on an electropneumatic piston, where the crystals are kept in a humidity and temperature-control...

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Detalles Bibliográficos
Autores principales: Mehrabi, Pedram, Sung, Sihyun, von Stetten, David, Prester, Andreas, Hatton, Caitlin E., Kleine-Döpke, Stephan, Berkes, Alexander, Gore, Gargi, Leimkohl, Jan-Philipp, Schikora, Hendrik, Kollewe, Martin, Rohde, Holger, Wilmanns, Matthias, Tellkamp, Friedjof, Schulz, Eike C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130016/
https://www.ncbi.nlm.nih.gov/pubmed/37185266
http://dx.doi.org/10.1038/s41467-023-37834-w

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