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Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis

The growth in world population, climate change, and resource scarcity necessitate a sustainable increase in crop productivity. Photosynthesis in major crops is limited by the inefficiency of the key CO(2)-fixing enzyme Rubisco, owing to its low carboxylation rate and poor ability to discriminate bet...

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Autores principales: Chen, Taiyu, Hojka, Marta, Davey, Philip, Sun, Yaqi, Dykes, Gregory F., Zhou, Fei, Lawson, Tracy, Nixon, Peter J., Lin, Yongjun, Liu, Lu-Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130085/
https://www.ncbi.nlm.nih.gov/pubmed/37185249
http://dx.doi.org/10.1038/s41467-023-37490-0
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author Chen, Taiyu
Hojka, Marta
Davey, Philip
Sun, Yaqi
Dykes, Gregory F.
Zhou, Fei
Lawson, Tracy
Nixon, Peter J.
Lin, Yongjun
Liu, Lu-Ning
author_facet Chen, Taiyu
Hojka, Marta
Davey, Philip
Sun, Yaqi
Dykes, Gregory F.
Zhou, Fei
Lawson, Tracy
Nixon, Peter J.
Lin, Yongjun
Liu, Lu-Ning
author_sort Chen, Taiyu
collection PubMed
description The growth in world population, climate change, and resource scarcity necessitate a sustainable increase in crop productivity. Photosynthesis in major crops is limited by the inefficiency of the key CO(2)-fixing enzyme Rubisco, owing to its low carboxylation rate and poor ability to discriminate between CO(2) and O(2). In cyanobacteria and proteobacteria, carboxysomes function as the central CO(2)-fixing organelles that elevate CO(2) levels around encapsulated Rubisco to enhance carboxylation. There is growing interest in engineering carboxysomes into crop chloroplasts as a potential route for improving photosynthesis and crop yields. Here, we generate morphologically correct carboxysomes in tobacco chloroplasts by transforming nine carboxysome genetic components derived from a proteobacterium. The chloroplast-expressed carboxysomes display a structural and functional integrity comparable to native carboxysomes and support autotrophic growth and photosynthesis of the transplastomic plants at elevated CO(2). Our study provides proof-of-concept for a route to engineering fully functional CO(2)-fixing modules and entire CO(2)-concentrating mechanisms into chloroplasts to improve crop photosynthesis and productivity.
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spelling pubmed-101300852023-04-27 Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis Chen, Taiyu Hojka, Marta Davey, Philip Sun, Yaqi Dykes, Gregory F. Zhou, Fei Lawson, Tracy Nixon, Peter J. Lin, Yongjun Liu, Lu-Ning Nat Commun Article The growth in world population, climate change, and resource scarcity necessitate a sustainable increase in crop productivity. Photosynthesis in major crops is limited by the inefficiency of the key CO(2)-fixing enzyme Rubisco, owing to its low carboxylation rate and poor ability to discriminate between CO(2) and O(2). In cyanobacteria and proteobacteria, carboxysomes function as the central CO(2)-fixing organelles that elevate CO(2) levels around encapsulated Rubisco to enhance carboxylation. There is growing interest in engineering carboxysomes into crop chloroplasts as a potential route for improving photosynthesis and crop yields. Here, we generate morphologically correct carboxysomes in tobacco chloroplasts by transforming nine carboxysome genetic components derived from a proteobacterium. The chloroplast-expressed carboxysomes display a structural and functional integrity comparable to native carboxysomes and support autotrophic growth and photosynthesis of the transplastomic plants at elevated CO(2). Our study provides proof-of-concept for a route to engineering fully functional CO(2)-fixing modules and entire CO(2)-concentrating mechanisms into chloroplasts to improve crop photosynthesis and productivity. Nature Publishing Group UK 2023-04-25 /pmc/articles/PMC10130085/ /pubmed/37185249 http://dx.doi.org/10.1038/s41467-023-37490-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Chen, Taiyu
Hojka, Marta
Davey, Philip
Sun, Yaqi
Dykes, Gregory F.
Zhou, Fei
Lawson, Tracy
Nixon, Peter J.
Lin, Yongjun
Liu, Lu-Ning
Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis
title Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis
title_full Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis
title_fullStr Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis
title_full_unstemmed Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis
title_short Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis
title_sort engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130085/
https://www.ncbi.nlm.nih.gov/pubmed/37185249
http://dx.doi.org/10.1038/s41467-023-37490-0
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