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Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis
The growth in world population, climate change, and resource scarcity necessitate a sustainable increase in crop productivity. Photosynthesis in major crops is limited by the inefficiency of the key CO(2)-fixing enzyme Rubisco, owing to its low carboxylation rate and poor ability to discriminate bet...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130085/ https://www.ncbi.nlm.nih.gov/pubmed/37185249 http://dx.doi.org/10.1038/s41467-023-37490-0 |
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author | Chen, Taiyu Hojka, Marta Davey, Philip Sun, Yaqi Dykes, Gregory F. Zhou, Fei Lawson, Tracy Nixon, Peter J. Lin, Yongjun Liu, Lu-Ning |
author_facet | Chen, Taiyu Hojka, Marta Davey, Philip Sun, Yaqi Dykes, Gregory F. Zhou, Fei Lawson, Tracy Nixon, Peter J. Lin, Yongjun Liu, Lu-Ning |
author_sort | Chen, Taiyu |
collection | PubMed |
description | The growth in world population, climate change, and resource scarcity necessitate a sustainable increase in crop productivity. Photosynthesis in major crops is limited by the inefficiency of the key CO(2)-fixing enzyme Rubisco, owing to its low carboxylation rate and poor ability to discriminate between CO(2) and O(2). In cyanobacteria and proteobacteria, carboxysomes function as the central CO(2)-fixing organelles that elevate CO(2) levels around encapsulated Rubisco to enhance carboxylation. There is growing interest in engineering carboxysomes into crop chloroplasts as a potential route for improving photosynthesis and crop yields. Here, we generate morphologically correct carboxysomes in tobacco chloroplasts by transforming nine carboxysome genetic components derived from a proteobacterium. The chloroplast-expressed carboxysomes display a structural and functional integrity comparable to native carboxysomes and support autotrophic growth and photosynthesis of the transplastomic plants at elevated CO(2). Our study provides proof-of-concept for a route to engineering fully functional CO(2)-fixing modules and entire CO(2)-concentrating mechanisms into chloroplasts to improve crop photosynthesis and productivity. |
format | Online Article Text |
id | pubmed-10130085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-101300852023-04-27 Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis Chen, Taiyu Hojka, Marta Davey, Philip Sun, Yaqi Dykes, Gregory F. Zhou, Fei Lawson, Tracy Nixon, Peter J. Lin, Yongjun Liu, Lu-Ning Nat Commun Article The growth in world population, climate change, and resource scarcity necessitate a sustainable increase in crop productivity. Photosynthesis in major crops is limited by the inefficiency of the key CO(2)-fixing enzyme Rubisco, owing to its low carboxylation rate and poor ability to discriminate between CO(2) and O(2). In cyanobacteria and proteobacteria, carboxysomes function as the central CO(2)-fixing organelles that elevate CO(2) levels around encapsulated Rubisco to enhance carboxylation. There is growing interest in engineering carboxysomes into crop chloroplasts as a potential route for improving photosynthesis and crop yields. Here, we generate morphologically correct carboxysomes in tobacco chloroplasts by transforming nine carboxysome genetic components derived from a proteobacterium. The chloroplast-expressed carboxysomes display a structural and functional integrity comparable to native carboxysomes and support autotrophic growth and photosynthesis of the transplastomic plants at elevated CO(2). Our study provides proof-of-concept for a route to engineering fully functional CO(2)-fixing modules and entire CO(2)-concentrating mechanisms into chloroplasts to improve crop photosynthesis and productivity. Nature Publishing Group UK 2023-04-25 /pmc/articles/PMC10130085/ /pubmed/37185249 http://dx.doi.org/10.1038/s41467-023-37490-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Chen, Taiyu Hojka, Marta Davey, Philip Sun, Yaqi Dykes, Gregory F. Zhou, Fei Lawson, Tracy Nixon, Peter J. Lin, Yongjun Liu, Lu-Ning Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis |
title | Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis |
title_full | Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis |
title_fullStr | Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis |
title_full_unstemmed | Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis |
title_short | Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis |
title_sort | engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130085/ https://www.ncbi.nlm.nih.gov/pubmed/37185249 http://dx.doi.org/10.1038/s41467-023-37490-0 |
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