Cargando…

The cerebrospinal fluid immune cell landscape in animal models of multiple sclerosis

The fluid compartment surrounding the central nervous system (CNS) is a unique source of immune cells capable of reflecting the pathophysiology of neurologic diseases. While human clinical and experimental studies often employ cerebrospinal fluid (CSF) analysis, assessment of CSF in animal models of...

Descripción completa

Detalles Bibliográficos
Autor principal: Wu, Gregory F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130411/
https://www.ncbi.nlm.nih.gov/pubmed/37122618
http://dx.doi.org/10.3389/fnmol.2023.1143498
_version_ 1785030951238107136
author Wu, Gregory F.
author_facet Wu, Gregory F.
author_sort Wu, Gregory F.
collection PubMed
description The fluid compartment surrounding the central nervous system (CNS) is a unique source of immune cells capable of reflecting the pathophysiology of neurologic diseases. While human clinical and experimental studies often employ cerebrospinal fluid (CSF) analysis, assessment of CSF in animal models of disease are wholly uncommon, particularly in examining the cellular component. Barriers to routine assessment of CSF in animal models of multiple sclerosis (MS) include limited sample volume, blood contamination, and lack of feasible longitudinal approaches. The few studies characterizing CSF immune cells in animal models of MS are largely outdated, but recent work employing transcriptomics have been used to explore new concepts in CNS inflammation and MS. Absence of extensive CSF data from rodent and other systems has curbed the overall impact of experimental models of MS. Future approaches, including examination of CSF myeloid subsets, single cell transcriptomics incorporating antigen receptor sequencing, and use of diverse animal models, may serve to overcome current limitations and provide critical insights into the pathogenesis of, and therapeutic developments for, MS.
format Online
Article
Text
id pubmed-10130411
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-101304112023-04-27 The cerebrospinal fluid immune cell landscape in animal models of multiple sclerosis Wu, Gregory F. Front Mol Neurosci Molecular Neuroscience The fluid compartment surrounding the central nervous system (CNS) is a unique source of immune cells capable of reflecting the pathophysiology of neurologic diseases. While human clinical and experimental studies often employ cerebrospinal fluid (CSF) analysis, assessment of CSF in animal models of disease are wholly uncommon, particularly in examining the cellular component. Barriers to routine assessment of CSF in animal models of multiple sclerosis (MS) include limited sample volume, blood contamination, and lack of feasible longitudinal approaches. The few studies characterizing CSF immune cells in animal models of MS are largely outdated, but recent work employing transcriptomics have been used to explore new concepts in CNS inflammation and MS. Absence of extensive CSF data from rodent and other systems has curbed the overall impact of experimental models of MS. Future approaches, including examination of CSF myeloid subsets, single cell transcriptomics incorporating antigen receptor sequencing, and use of diverse animal models, may serve to overcome current limitations and provide critical insights into the pathogenesis of, and therapeutic developments for, MS. Frontiers Media S.A. 2023-04-12 /pmc/articles/PMC10130411/ /pubmed/37122618 http://dx.doi.org/10.3389/fnmol.2023.1143498 Text en Copyright © 2023 Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Neuroscience
Wu, Gregory F.
The cerebrospinal fluid immune cell landscape in animal models of multiple sclerosis
title The cerebrospinal fluid immune cell landscape in animal models of multiple sclerosis
title_full The cerebrospinal fluid immune cell landscape in animal models of multiple sclerosis
title_fullStr The cerebrospinal fluid immune cell landscape in animal models of multiple sclerosis
title_full_unstemmed The cerebrospinal fluid immune cell landscape in animal models of multiple sclerosis
title_short The cerebrospinal fluid immune cell landscape in animal models of multiple sclerosis
title_sort cerebrospinal fluid immune cell landscape in animal models of multiple sclerosis
topic Molecular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130411/
https://www.ncbi.nlm.nih.gov/pubmed/37122618
http://dx.doi.org/10.3389/fnmol.2023.1143498
work_keys_str_mv AT wugregoryf thecerebrospinalfluidimmunecelllandscapeinanimalmodelsofmultiplesclerosis
AT wugregoryf cerebrospinalfluidimmunecelllandscapeinanimalmodelsofmultiplesclerosis