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Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells
Sargassum horneri (S. horneri) is a brown seaweed that contains a fucose-rich sulfated polysaccharide called fucoidan and is known to possess beneficial bioactivities, such as anti-inflammatory, antiviral, antioxidative, and antitumoral effects. This study aimed to determine the anti-inflammatory ef...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130517/ https://www.ncbi.nlm.nih.gov/pubmed/37125026 http://dx.doi.org/10.3389/fnut.2023.1162934 |
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author | Lee, Sol Lee, Eun Jeoung Lee, Gyu Min Yun, Ji-Hyun Yoo, Wonbeak |
author_facet | Lee, Sol Lee, Eun Jeoung Lee, Gyu Min Yun, Ji-Hyun Yoo, Wonbeak |
author_sort | Lee, Sol |
collection | PubMed |
description | Sargassum horneri (S. horneri) is a brown seaweed that contains a fucose-rich sulfated polysaccharide called fucoidan and is known to possess beneficial bioactivities, such as anti-inflammatory, antiviral, antioxidative, and antitumoral effects. This study aimed to determine the anti-inflammatory effects of AB_SH (hydrothermal extracts from S. horneri) and its bioactive compound (fucoidan) against tumor necrosis factor alpha (TNF-α)-induced inflammation in human retinal pigment epithelial (RPE) cells. AB_SH did not exhibit any cytotoxicity, and it decreased the mRNA expression of interleukin (IL)-6 and IL-8 and the production of the cytokines IL-6 and TNF-α. It also suppressed the expression levels of phosphorylated nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs), including c-Jun amino-terminal kinases (JNK), p38 protein kinases (p38), and extracellular signal-regulated kinase (ERK) proteins, suggesting that AB_SH inhibits activation of the NF-kB/MAPK signaling pathway. Since fucoidan was identified in the composition analysis of AB_SH, it was additionally shown to be required for its anti-inflammatory effects in TNF-α-stimulated human RPE cells. In line with the AB_SH results, fucoidan reduced the mRNA levels of IL-6, IL-1ß, and IL-8 and production of the cytokines IL-6, TNF-α, and IL-8 through the downregulation of the NF-kB/MAPK signaling pathway in a dose-dependent manner. Collectively, the ability of AB_SH from S. horneri hydrothermal extracts to reduce inflammation indicates that it may be a good functional ingredient for managing ocular disorders. |
format | Online Article Text |
id | pubmed-10130517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101305172023-04-27 Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells Lee, Sol Lee, Eun Jeoung Lee, Gyu Min Yun, Ji-Hyun Yoo, Wonbeak Front Nutr Nutrition Sargassum horneri (S. horneri) is a brown seaweed that contains a fucose-rich sulfated polysaccharide called fucoidan and is known to possess beneficial bioactivities, such as anti-inflammatory, antiviral, antioxidative, and antitumoral effects. This study aimed to determine the anti-inflammatory effects of AB_SH (hydrothermal extracts from S. horneri) and its bioactive compound (fucoidan) against tumor necrosis factor alpha (TNF-α)-induced inflammation in human retinal pigment epithelial (RPE) cells. AB_SH did not exhibit any cytotoxicity, and it decreased the mRNA expression of interleukin (IL)-6 and IL-8 and the production of the cytokines IL-6 and TNF-α. It also suppressed the expression levels of phosphorylated nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs), including c-Jun amino-terminal kinases (JNK), p38 protein kinases (p38), and extracellular signal-regulated kinase (ERK) proteins, suggesting that AB_SH inhibits activation of the NF-kB/MAPK signaling pathway. Since fucoidan was identified in the composition analysis of AB_SH, it was additionally shown to be required for its anti-inflammatory effects in TNF-α-stimulated human RPE cells. In line with the AB_SH results, fucoidan reduced the mRNA levels of IL-6, IL-1ß, and IL-8 and production of the cytokines IL-6, TNF-α, and IL-8 through the downregulation of the NF-kB/MAPK signaling pathway in a dose-dependent manner. Collectively, the ability of AB_SH from S. horneri hydrothermal extracts to reduce inflammation indicates that it may be a good functional ingredient for managing ocular disorders. Frontiers Media S.A. 2023-04-12 /pmc/articles/PMC10130517/ /pubmed/37125026 http://dx.doi.org/10.3389/fnut.2023.1162934 Text en Copyright © 2023 Lee, Lee, Lee, Yun and Yoo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Lee, Sol Lee, Eun Jeoung Lee, Gyu Min Yun, Ji-Hyun Yoo, Wonbeak Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells |
title | Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells |
title_full | Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells |
title_fullStr | Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells |
title_full_unstemmed | Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells |
title_short | Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells |
title_sort | inhibitory effect of fucoidan on tnf-α-induced inflammation in human retinal pigment epithelium cells |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130517/ https://www.ncbi.nlm.nih.gov/pubmed/37125026 http://dx.doi.org/10.3389/fnut.2023.1162934 |
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