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Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells

Sargassum horneri (S. horneri) is a brown seaweed that contains a fucose-rich sulfated polysaccharide called fucoidan and is known to possess beneficial bioactivities, such as anti-inflammatory, antiviral, antioxidative, and antitumoral effects. This study aimed to determine the anti-inflammatory ef...

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Autores principales: Lee, Sol, Lee, Eun Jeoung, Lee, Gyu Min, Yun, Ji-Hyun, Yoo, Wonbeak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130517/
https://www.ncbi.nlm.nih.gov/pubmed/37125026
http://dx.doi.org/10.3389/fnut.2023.1162934
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author Lee, Sol
Lee, Eun Jeoung
Lee, Gyu Min
Yun, Ji-Hyun
Yoo, Wonbeak
author_facet Lee, Sol
Lee, Eun Jeoung
Lee, Gyu Min
Yun, Ji-Hyun
Yoo, Wonbeak
author_sort Lee, Sol
collection PubMed
description Sargassum horneri (S. horneri) is a brown seaweed that contains a fucose-rich sulfated polysaccharide called fucoidan and is known to possess beneficial bioactivities, such as anti-inflammatory, antiviral, antioxidative, and antitumoral effects. This study aimed to determine the anti-inflammatory effects of AB_SH (hydrothermal extracts from S. horneri) and its bioactive compound (fucoidan) against tumor necrosis factor alpha (TNF-α)-induced inflammation in human retinal pigment epithelial (RPE) cells. AB_SH did not exhibit any cytotoxicity, and it decreased the mRNA expression of interleukin (IL)-6 and IL-8 and the production of the cytokines IL-6 and TNF-α. It also suppressed the expression levels of phosphorylated nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs), including c-Jun amino-terminal kinases (JNK), p38 protein kinases (p38), and extracellular signal-regulated kinase (ERK) proteins, suggesting that AB_SH inhibits activation of the NF-kB/MAPK signaling pathway. Since fucoidan was identified in the composition analysis of AB_SH, it was additionally shown to be required for its anti-inflammatory effects in TNF-α-stimulated human RPE cells. In line with the AB_SH results, fucoidan reduced the mRNA levels of IL-6, IL-1ß, and IL-8 and production of the cytokines IL-6, TNF-α, and IL-8 through the downregulation of the NF-kB/MAPK signaling pathway in a dose-dependent manner. Collectively, the ability of AB_SH from S. horneri hydrothermal extracts to reduce inflammation indicates that it may be a good functional ingredient for managing ocular disorders.
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spelling pubmed-101305172023-04-27 Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells Lee, Sol Lee, Eun Jeoung Lee, Gyu Min Yun, Ji-Hyun Yoo, Wonbeak Front Nutr Nutrition Sargassum horneri (S. horneri) is a brown seaweed that contains a fucose-rich sulfated polysaccharide called fucoidan and is known to possess beneficial bioactivities, such as anti-inflammatory, antiviral, antioxidative, and antitumoral effects. This study aimed to determine the anti-inflammatory effects of AB_SH (hydrothermal extracts from S. horneri) and its bioactive compound (fucoidan) against tumor necrosis factor alpha (TNF-α)-induced inflammation in human retinal pigment epithelial (RPE) cells. AB_SH did not exhibit any cytotoxicity, and it decreased the mRNA expression of interleukin (IL)-6 and IL-8 and the production of the cytokines IL-6 and TNF-α. It also suppressed the expression levels of phosphorylated nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs), including c-Jun amino-terminal kinases (JNK), p38 protein kinases (p38), and extracellular signal-regulated kinase (ERK) proteins, suggesting that AB_SH inhibits activation of the NF-kB/MAPK signaling pathway. Since fucoidan was identified in the composition analysis of AB_SH, it was additionally shown to be required for its anti-inflammatory effects in TNF-α-stimulated human RPE cells. In line with the AB_SH results, fucoidan reduced the mRNA levels of IL-6, IL-1ß, and IL-8 and production of the cytokines IL-6, TNF-α, and IL-8 through the downregulation of the NF-kB/MAPK signaling pathway in a dose-dependent manner. Collectively, the ability of AB_SH from S. horneri hydrothermal extracts to reduce inflammation indicates that it may be a good functional ingredient for managing ocular disorders. Frontiers Media S.A. 2023-04-12 /pmc/articles/PMC10130517/ /pubmed/37125026 http://dx.doi.org/10.3389/fnut.2023.1162934 Text en Copyright © 2023 Lee, Lee, Lee, Yun and Yoo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Lee, Sol
Lee, Eun Jeoung
Lee, Gyu Min
Yun, Ji-Hyun
Yoo, Wonbeak
Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells
title Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells
title_full Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells
title_fullStr Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells
title_full_unstemmed Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells
title_short Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells
title_sort inhibitory effect of fucoidan on tnf-α-induced inflammation in human retinal pigment epithelium cells
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130517/
https://www.ncbi.nlm.nih.gov/pubmed/37125026
http://dx.doi.org/10.3389/fnut.2023.1162934
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