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The environmental stress response regulates ribosome content in cell cycle-arrested S. cerevisiae
Prolonged cell cycle arrests occur naturally in differentiated cells and in response to various stresses such as nutrient deprivation or treatment with chemotherapeutic agents. Whether and how cells survive prolonged cell cycle arrests is not clear. Here, we used S. cerevisiae to compare physiologic...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130656/ https://www.ncbi.nlm.nih.gov/pubmed/37123399 http://dx.doi.org/10.3389/fcell.2023.1118766 |
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author | Terhorst, Allegra Sandikci, Arzu Whittaker, Charles A. Szórádi, Tamás Holt, Liam J. Neurohr, Gabriel E. Amon, Angelika |
author_facet | Terhorst, Allegra Sandikci, Arzu Whittaker, Charles A. Szórádi, Tamás Holt, Liam J. Neurohr, Gabriel E. Amon, Angelika |
author_sort | Terhorst, Allegra |
collection | PubMed |
description | Prolonged cell cycle arrests occur naturally in differentiated cells and in response to various stresses such as nutrient deprivation or treatment with chemotherapeutic agents. Whether and how cells survive prolonged cell cycle arrests is not clear. Here, we used S. cerevisiae to compare physiological cell cycle arrests and genetically induced arrests in G1-, meta- and anaphase. Prolonged cell cycle arrest led to growth attenuation in all studied conditions, coincided with activation of the Environmental Stress Response (ESR) and with a reduced ribosome content as determined by whole ribosome purification and TMT mass spectrometry. Suppression of the ESR through hyperactivation of the Ras/PKA pathway reduced cell viability during prolonged arrests, demonstrating a cytoprotective role of the ESR. Attenuation of cell growth and activation of stress induced signaling pathways also occur in arrested human cell lines, raising the possibility that the response to prolonged cell cycle arrest is conserved. |
format | Online Article Text |
id | pubmed-10130656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101306562023-04-27 The environmental stress response regulates ribosome content in cell cycle-arrested S. cerevisiae Terhorst, Allegra Sandikci, Arzu Whittaker, Charles A. Szórádi, Tamás Holt, Liam J. Neurohr, Gabriel E. Amon, Angelika Front Cell Dev Biol Cell and Developmental Biology Prolonged cell cycle arrests occur naturally in differentiated cells and in response to various stresses such as nutrient deprivation or treatment with chemotherapeutic agents. Whether and how cells survive prolonged cell cycle arrests is not clear. Here, we used S. cerevisiae to compare physiological cell cycle arrests and genetically induced arrests in G1-, meta- and anaphase. Prolonged cell cycle arrest led to growth attenuation in all studied conditions, coincided with activation of the Environmental Stress Response (ESR) and with a reduced ribosome content as determined by whole ribosome purification and TMT mass spectrometry. Suppression of the ESR through hyperactivation of the Ras/PKA pathway reduced cell viability during prolonged arrests, demonstrating a cytoprotective role of the ESR. Attenuation of cell growth and activation of stress induced signaling pathways also occur in arrested human cell lines, raising the possibility that the response to prolonged cell cycle arrest is conserved. Frontiers Media S.A. 2023-04-12 /pmc/articles/PMC10130656/ /pubmed/37123399 http://dx.doi.org/10.3389/fcell.2023.1118766 Text en Copyright © 2023 Terhorst, Sandikci, Whittaker, Szórádi, Holt, Neurohr and Amon. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Terhorst, Allegra Sandikci, Arzu Whittaker, Charles A. Szórádi, Tamás Holt, Liam J. Neurohr, Gabriel E. Amon, Angelika The environmental stress response regulates ribosome content in cell cycle-arrested S. cerevisiae |
title | The environmental stress response regulates ribosome content in cell cycle-arrested S. cerevisiae
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title_full | The environmental stress response regulates ribosome content in cell cycle-arrested S. cerevisiae
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title_fullStr | The environmental stress response regulates ribosome content in cell cycle-arrested S. cerevisiae
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title_full_unstemmed | The environmental stress response regulates ribosome content in cell cycle-arrested S. cerevisiae
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title_short | The environmental stress response regulates ribosome content in cell cycle-arrested S. cerevisiae
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title_sort | environmental stress response regulates ribosome content in cell cycle-arrested s. cerevisiae |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130656/ https://www.ncbi.nlm.nih.gov/pubmed/37123399 http://dx.doi.org/10.3389/fcell.2023.1118766 |
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