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Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair
Crosstalk between nerves and bone is essential for bone repair, for which Schwann cells (SCs) are crucial in the regulation of the microenvironment. Considering that exosomes are critical paracrine mediators for intercellular communication that exert important effects in tissue repair, the aim of th...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10131128/ https://www.ncbi.nlm.nih.gov/pubmed/37122897 http://dx.doi.org/10.1016/j.bioactmat.2023.02.013 |
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author | Wang, Tianchang Li, Wentao Zhang, Yuxin Xu, Xiang Qiang, Lei Miao, Weiqiang Yue, Xiaokun Jiao, Xin Zhou, Xianhao Ma, Zhenjiang Li, Shuai Ding, Muliang Zhu, Junfeng Yang, Chi Wang, Hui Li, Tao Sun, Xin Wang, Jinwu |
author_facet | Wang, Tianchang Li, Wentao Zhang, Yuxin Xu, Xiang Qiang, Lei Miao, Weiqiang Yue, Xiaokun Jiao, Xin Zhou, Xianhao Ma, Zhenjiang Li, Shuai Ding, Muliang Zhu, Junfeng Yang, Chi Wang, Hui Li, Tao Sun, Xin Wang, Jinwu |
author_sort | Wang, Tianchang |
collection | PubMed |
description | Crosstalk between nerves and bone is essential for bone repair, for which Schwann cells (SCs) are crucial in the regulation of the microenvironment. Considering that exosomes are critical paracrine mediators for intercellular communication that exert important effects in tissue repair, the aim of this study is to confirm the function and molecular mechanisms of Schwann cell-derived exosomes (SC-exos) on bone regeneration and to propose engineered constructs that simulate SC-mediated nerve–bone crosstalk. SCs promoted the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs) through exosomes. Subsequent molecular mechanism studies demonstrated that SC-exos promoted BMSC osteogenesis by regulating the TGF-β signaling pathway via let-7c-5p. Interestingly, SC-exos promoted the migration and tube formation performance of endothelial progenitor cells. Furthermore, the SC-exos@G/S constructs were developed by bioprinting technology that simulated SC-mediated nerve–bone crosstalk and improved the bone regeneration microenvironment by releasing SC-exos, exerting the regulatory effect of SCs in the microenvironment to promote innervation, vascularization, and osteogenesis and thus effectively improving bone repair in a cranial defect model. This study demonstrates the important role and underlying mechanism of SCs in regulating bone regeneration through SC-exos and provides a new engineered strategy for bone repair. |
format | Online Article Text |
id | pubmed-10131128 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-101311282023-04-27 Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair Wang, Tianchang Li, Wentao Zhang, Yuxin Xu, Xiang Qiang, Lei Miao, Weiqiang Yue, Xiaokun Jiao, Xin Zhou, Xianhao Ma, Zhenjiang Li, Shuai Ding, Muliang Zhu, Junfeng Yang, Chi Wang, Hui Li, Tao Sun, Xin Wang, Jinwu Bioact Mater Article Crosstalk between nerves and bone is essential for bone repair, for which Schwann cells (SCs) are crucial in the regulation of the microenvironment. Considering that exosomes are critical paracrine mediators for intercellular communication that exert important effects in tissue repair, the aim of this study is to confirm the function and molecular mechanisms of Schwann cell-derived exosomes (SC-exos) on bone regeneration and to propose engineered constructs that simulate SC-mediated nerve–bone crosstalk. SCs promoted the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs) through exosomes. Subsequent molecular mechanism studies demonstrated that SC-exos promoted BMSC osteogenesis by regulating the TGF-β signaling pathway via let-7c-5p. Interestingly, SC-exos promoted the migration and tube formation performance of endothelial progenitor cells. Furthermore, the SC-exos@G/S constructs were developed by bioprinting technology that simulated SC-mediated nerve–bone crosstalk and improved the bone regeneration microenvironment by releasing SC-exos, exerting the regulatory effect of SCs in the microenvironment to promote innervation, vascularization, and osteogenesis and thus effectively improving bone repair in a cranial defect model. This study demonstrates the important role and underlying mechanism of SCs in regulating bone regeneration through SC-exos and provides a new engineered strategy for bone repair. KeAi Publishing 2023-04-21 /pmc/articles/PMC10131128/ /pubmed/37122897 http://dx.doi.org/10.1016/j.bioactmat.2023.02.013 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Wang, Tianchang Li, Wentao Zhang, Yuxin Xu, Xiang Qiang, Lei Miao, Weiqiang Yue, Xiaokun Jiao, Xin Zhou, Xianhao Ma, Zhenjiang Li, Shuai Ding, Muliang Zhu, Junfeng Yang, Chi Wang, Hui Li, Tao Sun, Xin Wang, Jinwu Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair |
title | Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair |
title_full | Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair |
title_fullStr | Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair |
title_full_unstemmed | Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair |
title_short | Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair |
title_sort | bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10131128/ https://www.ncbi.nlm.nih.gov/pubmed/37122897 http://dx.doi.org/10.1016/j.bioactmat.2023.02.013 |
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