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Protocol optimization and assessment of genotypic response for inbred line development through doubled haploid production in maize

BACKGROUND: Doubled haploid technology offers the fastest route of inbred line development by rapidly fixing the desirable combinations in a single year. However, the differential response of haploid induction to genetic background of maternal lines accompanied with low induction rate and high morta...

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Autores principales: Kaur, Harjot, Kyum, Mohammed, Sandhu, Surinder, Singh, Gagandeep, Sharma, Priti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10131367/
https://www.ncbi.nlm.nih.gov/pubmed/37098500
http://dx.doi.org/10.1186/s12870-023-04228-5
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author Kaur, Harjot
Kyum, Mohammed
Sandhu, Surinder
Singh, Gagandeep
Sharma, Priti
author_facet Kaur, Harjot
Kyum, Mohammed
Sandhu, Surinder
Singh, Gagandeep
Sharma, Priti
author_sort Kaur, Harjot
collection PubMed
description BACKGROUND: Doubled haploid technology offers the fastest route of inbred line development by rapidly fixing the desirable combinations in a single year. However, the differential response of haploid induction to genetic background of maternal lines accompanied with low induction rate and high mortality rate due to artificial chromosomal doubling of haploid seedlings creates hindrance in doubled haploid production on a commercial scale under tropical conditions. To speed up the hybrid breeding programme in sub-tropical maize, efforts are reported here to optimize the protocol for efficient production of fixed lines using haploid inducers. The second-generation haploid inducers i.e. CIM2GTAILs obtained from CIMMYT, Mexico were used for haploid induction in 13 F(1)s of diverse backgrounds. For standardization of chromosomal doubling protocol, various concentrations of colchicine and two seedling growth stages were used to determine the extent of chromosomal doubling and survival rate of doubled haploid plants. RESULTS: A high mean haploid induction rate is obtained from CIM2GTAIL P2 (10%) as compared to CIM2GTAIL P1 (7.46%). Out of four treatments, CIMMYT reported protocol of chromosome doubling in tropical maize comprising combination of 0.07% colchicine and 0.1% DMSO at V(2) stage is highly effective for acquiring doubled haploid plants in sub-tropical adapted maize with high survival rate of 52.7%. However, increasing the colchicine concentration from 0.07 to 0.1% led to high mortality rate. CONCLUSION: According to the findings, the haploid induction rate, survival rate and overall success rate varied depending upon the genotype of the inducer and the source population along with the concentrations of chemical used. The optimized protocol developed using CIMMYT haploid inducer CIM2GTAIL P2 for efficient doubled haploid production will not only fasten the breeding programme but will also reduce the production cost of doubled haploid with great efficiency in sub-tropical maize.
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spelling pubmed-101313672023-04-27 Protocol optimization and assessment of genotypic response for inbred line development through doubled haploid production in maize Kaur, Harjot Kyum, Mohammed Sandhu, Surinder Singh, Gagandeep Sharma, Priti BMC Plant Biol Research BACKGROUND: Doubled haploid technology offers the fastest route of inbred line development by rapidly fixing the desirable combinations in a single year. However, the differential response of haploid induction to genetic background of maternal lines accompanied with low induction rate and high mortality rate due to artificial chromosomal doubling of haploid seedlings creates hindrance in doubled haploid production on a commercial scale under tropical conditions. To speed up the hybrid breeding programme in sub-tropical maize, efforts are reported here to optimize the protocol for efficient production of fixed lines using haploid inducers. The second-generation haploid inducers i.e. CIM2GTAILs obtained from CIMMYT, Mexico were used for haploid induction in 13 F(1)s of diverse backgrounds. For standardization of chromosomal doubling protocol, various concentrations of colchicine and two seedling growth stages were used to determine the extent of chromosomal doubling and survival rate of doubled haploid plants. RESULTS: A high mean haploid induction rate is obtained from CIM2GTAIL P2 (10%) as compared to CIM2GTAIL P1 (7.46%). Out of four treatments, CIMMYT reported protocol of chromosome doubling in tropical maize comprising combination of 0.07% colchicine and 0.1% DMSO at V(2) stage is highly effective for acquiring doubled haploid plants in sub-tropical adapted maize with high survival rate of 52.7%. However, increasing the colchicine concentration from 0.07 to 0.1% led to high mortality rate. CONCLUSION: According to the findings, the haploid induction rate, survival rate and overall success rate varied depending upon the genotype of the inducer and the source population along with the concentrations of chemical used. The optimized protocol developed using CIMMYT haploid inducer CIM2GTAIL P2 for efficient doubled haploid production will not only fasten the breeding programme but will also reduce the production cost of doubled haploid with great efficiency in sub-tropical maize. BioMed Central 2023-04-26 /pmc/articles/PMC10131367/ /pubmed/37098500 http://dx.doi.org/10.1186/s12870-023-04228-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kaur, Harjot
Kyum, Mohammed
Sandhu, Surinder
Singh, Gagandeep
Sharma, Priti
Protocol optimization and assessment of genotypic response for inbred line development through doubled haploid production in maize
title Protocol optimization and assessment of genotypic response for inbred line development through doubled haploid production in maize
title_full Protocol optimization and assessment of genotypic response for inbred line development through doubled haploid production in maize
title_fullStr Protocol optimization and assessment of genotypic response for inbred line development through doubled haploid production in maize
title_full_unstemmed Protocol optimization and assessment of genotypic response for inbred line development through doubled haploid production in maize
title_short Protocol optimization and assessment of genotypic response for inbred line development through doubled haploid production in maize
title_sort protocol optimization and assessment of genotypic response for inbred line development through doubled haploid production in maize
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10131367/
https://www.ncbi.nlm.nih.gov/pubmed/37098500
http://dx.doi.org/10.1186/s12870-023-04228-5
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