Beadchip technology to detect DNA methylation in mouse faithfully recapitulates whole-genome bisulfite sequencing

AIM: To facilitate wide-scale implementation of Illumina Mouse Methylation BeadChip (MMB) technology, array-based measurement of cytosine methylation was compared with the gold-standard assessment of DNA methylation by whole-genome bisulfite sequencing (WGBS). METHODS: DNA methylation across two mou...

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Detalles Bibliográficos
Autores principales: Martin, Elizabeth M, Grimm, Sara A, Xu, Zongli, Taylor, Jack A, Wade, Paul A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Medicine Ltd 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10131490/
https://www.ncbi.nlm.nih.gov/pubmed/37020391
http://dx.doi.org/10.2217/epi-2023-0034
Descripción
Sumario:AIM: To facilitate wide-scale implementation of Illumina Mouse Methylation BeadChip (MMB) technology, array-based measurement of cytosine methylation was compared with the gold-standard assessment of DNA methylation by whole-genome bisulfite sequencing (WGBS). METHODS: DNA methylation across two mouse strains (C57B6 and C3H) and both sexes was assessed using the MMB and compared with previously existing deep-coverage WGBS of mice of the same strain and sex. RESULTS & CONCLUSION: The findings demonstrated that 93.3–99.2% of sites had similar measurements of methylation across technologies and that differentially methylated cytosines and regions identified by each technology overlap and enrich for similar biological functions, suggesting that the MMB faithfully recapitulates the findings of WGBS.

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