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Glutamine Metabolism Underlies the Functional Similarity of T Cells between Nile Tilapia and Tetrapod
As the lowest organisms possessing T cells, fish are instrumental for understanding T cell evolution and immune defense in early vertebrates. This study established in Nile tilapia models suggests that T cells play a critical role in resisting Edwardsiella piscicida infection via cytotoxicity and ar...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10131875/ https://www.ncbi.nlm.nih.gov/pubmed/36890649 http://dx.doi.org/10.1002/advs.202201164 |
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author | Li, Kang Wei, Xiumei Jiao, Xinying Deng, Wenhai Li, Jiaqi Liang, Wei Zhang, Yu Yang, Jialong |
author_facet | Li, Kang Wei, Xiumei Jiao, Xinying Deng, Wenhai Li, Jiaqi Liang, Wei Zhang, Yu Yang, Jialong |
author_sort | Li, Kang |
collection | PubMed |
description | As the lowest organisms possessing T cells, fish are instrumental for understanding T cell evolution and immune defense in early vertebrates. This study established in Nile tilapia models suggests that T cells play a critical role in resisting Edwardsiella piscicida infection via cytotoxicity and are essential for IgM(+) B cell response. CD3 and CD28 monoclonal antibody crosslinking reveals that full activation of tilapia T cells requires the first and secondary signals, while Ca(2+)–NFAT, MAPK/ERK, NF‐κB, and mTORC1 pathways and IgM(+) B cells collectively regulate T cell activation. Thus, despite the large evolutionary distance, tilapia and mammals such as mice and humans exhibit similar T cell functions. Furthermore, it is speculated that transcriptional networks and metabolic reprogramming, especially c‐Myc‐mediated glutamine metabolism triggered by mTORC1 and MAPK/ERK pathways, underlie the functional similarity of T cells between tilapia and mammals. Notably, tilapia, frogs, chickens, and mice utilize the same mechanisms to facilitate glutaminolysis‐regulated T cell responses, and restoration of the glutaminolysis pathway using tilapia components rescues the immunodeficiency of human Jurkat T cells. Thus, this study provides a comprehensive picture of T cell immunity in tilapia, sheds novel perspectives for understanding T cell evolution, and offers potential avenues for intervening in human immunodeficiency. |
format | Online Article Text |
id | pubmed-10131875 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101318752023-04-27 Glutamine Metabolism Underlies the Functional Similarity of T Cells between Nile Tilapia and Tetrapod Li, Kang Wei, Xiumei Jiao, Xinying Deng, Wenhai Li, Jiaqi Liang, Wei Zhang, Yu Yang, Jialong Adv Sci (Weinh) Research Articles As the lowest organisms possessing T cells, fish are instrumental for understanding T cell evolution and immune defense in early vertebrates. This study established in Nile tilapia models suggests that T cells play a critical role in resisting Edwardsiella piscicida infection via cytotoxicity and are essential for IgM(+) B cell response. CD3 and CD28 monoclonal antibody crosslinking reveals that full activation of tilapia T cells requires the first and secondary signals, while Ca(2+)–NFAT, MAPK/ERK, NF‐κB, and mTORC1 pathways and IgM(+) B cells collectively regulate T cell activation. Thus, despite the large evolutionary distance, tilapia and mammals such as mice and humans exhibit similar T cell functions. Furthermore, it is speculated that transcriptional networks and metabolic reprogramming, especially c‐Myc‐mediated glutamine metabolism triggered by mTORC1 and MAPK/ERK pathways, underlie the functional similarity of T cells between tilapia and mammals. Notably, tilapia, frogs, chickens, and mice utilize the same mechanisms to facilitate glutaminolysis‐regulated T cell responses, and restoration of the glutaminolysis pathway using tilapia components rescues the immunodeficiency of human Jurkat T cells. Thus, this study provides a comprehensive picture of T cell immunity in tilapia, sheds novel perspectives for understanding T cell evolution, and offers potential avenues for intervening in human immunodeficiency. John Wiley and Sons Inc. 2023-03-08 /pmc/articles/PMC10131875/ /pubmed/36890649 http://dx.doi.org/10.1002/advs.202201164 Text en © 2023 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Li, Kang Wei, Xiumei Jiao, Xinying Deng, Wenhai Li, Jiaqi Liang, Wei Zhang, Yu Yang, Jialong Glutamine Metabolism Underlies the Functional Similarity of T Cells between Nile Tilapia and Tetrapod |
title | Glutamine Metabolism Underlies the Functional Similarity of T Cells between Nile Tilapia and Tetrapod |
title_full | Glutamine Metabolism Underlies the Functional Similarity of T Cells between Nile Tilapia and Tetrapod |
title_fullStr | Glutamine Metabolism Underlies the Functional Similarity of T Cells between Nile Tilapia and Tetrapod |
title_full_unstemmed | Glutamine Metabolism Underlies the Functional Similarity of T Cells between Nile Tilapia and Tetrapod |
title_short | Glutamine Metabolism Underlies the Functional Similarity of T Cells between Nile Tilapia and Tetrapod |
title_sort | glutamine metabolism underlies the functional similarity of t cells between nile tilapia and tetrapod |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10131875/ https://www.ncbi.nlm.nih.gov/pubmed/36890649 http://dx.doi.org/10.1002/advs.202201164 |
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