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Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16
Salmonella enterica serovar Paratyphi A (S. Paratyphi A) is a pathogen that can cause enteric fever. According to the recent epidemic trends of typhoid fever, S. Paratyphi A has been the major important causative factor in paratyphoid fever. An effective vaccine for S. Paratyphi A has not been devel...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10132459/ https://www.ncbi.nlm.nih.gov/pubmed/37122724 http://dx.doi.org/10.3389/fimmu.2023.1118003 |
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author | Ding, Ai-Jun Zhang, Wei-Ming Tao, Jian Chen, Bing Liu, Xiao-Cao Dong, Yu Ma, Han-Jing Pan, Shao-Dong He, Jiang-Bo Zeng, Wei-Kun |
author_facet | Ding, Ai-Jun Zhang, Wei-Ming Tao, Jian Chen, Bing Liu, Xiao-Cao Dong, Yu Ma, Han-Jing Pan, Shao-Dong He, Jiang-Bo Zeng, Wei-Kun |
author_sort | Ding, Ai-Jun |
collection | PubMed |
description | Salmonella enterica serovar Paratyphi A (S. Paratyphi A) is a pathogen that can cause enteric fever. According to the recent epidemic trends of typhoid fever, S. Paratyphi A has been the major important causative factor in paratyphoid fever. An effective vaccine for S. Paratyphi A has not been developed, which made it a tricky public health concern. Until now, how S. Paratyphi A interacts with organisms remain unknown. Here using lifespan assay, we found that S. Paratyphi A could infect Caenorhabditis elegans (C. elegans) at 25°C, and attenuate thermotolerance. The immune response of C. elegans was mediated by tir-1, nsy-1, sek-1, pmk-1, mpk-1, skn-1, daf-2 and daf-16, suggesting that S. Paratyphi A could regulate the MAPK and insulin pathways. Furthermore, we observed several phenotypical changes when C. elegans were fed S. Paratyphi A, including an accelerated decline in body movement, reduced the reproductive capacity, shortened spawning cycle, strong preference for OP50, arrested pharyngeal pumping and colonization of the intestinal lumen. The virulence of S. Paratyphi A requires living bacteria and is not mediated by secreting toxin. Using hydrogen peroxide analysis and quantitative RT-PCR, we discovered that S. Paratyphi A could increase oxidative stress and regulate the immune response in C. elegans. Our results sheds light on the infection mechanisms of S. Paratyphi A and lays a foundation for drugs and vaccine development. |
format | Online Article Text |
id | pubmed-10132459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101324592023-04-27 Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16 Ding, Ai-Jun Zhang, Wei-Ming Tao, Jian Chen, Bing Liu, Xiao-Cao Dong, Yu Ma, Han-Jing Pan, Shao-Dong He, Jiang-Bo Zeng, Wei-Kun Front Immunol Immunology Salmonella enterica serovar Paratyphi A (S. Paratyphi A) is a pathogen that can cause enteric fever. According to the recent epidemic trends of typhoid fever, S. Paratyphi A has been the major important causative factor in paratyphoid fever. An effective vaccine for S. Paratyphi A has not been developed, which made it a tricky public health concern. Until now, how S. Paratyphi A interacts with organisms remain unknown. Here using lifespan assay, we found that S. Paratyphi A could infect Caenorhabditis elegans (C. elegans) at 25°C, and attenuate thermotolerance. The immune response of C. elegans was mediated by tir-1, nsy-1, sek-1, pmk-1, mpk-1, skn-1, daf-2 and daf-16, suggesting that S. Paratyphi A could regulate the MAPK and insulin pathways. Furthermore, we observed several phenotypical changes when C. elegans were fed S. Paratyphi A, including an accelerated decline in body movement, reduced the reproductive capacity, shortened spawning cycle, strong preference for OP50, arrested pharyngeal pumping and colonization of the intestinal lumen. The virulence of S. Paratyphi A requires living bacteria and is not mediated by secreting toxin. Using hydrogen peroxide analysis and quantitative RT-PCR, we discovered that S. Paratyphi A could increase oxidative stress and regulate the immune response in C. elegans. Our results sheds light on the infection mechanisms of S. Paratyphi A and lays a foundation for drugs and vaccine development. Frontiers Media S.A. 2023-04-12 /pmc/articles/PMC10132459/ /pubmed/37122724 http://dx.doi.org/10.3389/fimmu.2023.1118003 Text en Copyright © 2023 Ding, Zhang, Tao, Chen, Liu, Dong, Ma, Pan, He and Zeng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Ding, Ai-Jun Zhang, Wei-Ming Tao, Jian Chen, Bing Liu, Xiao-Cao Dong, Yu Ma, Han-Jing Pan, Shao-Dong He, Jiang-Bo Zeng, Wei-Kun Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16 |
title |
Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16 |
title_full |
Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16 |
title_fullStr |
Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16 |
title_full_unstemmed |
Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16 |
title_short |
Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16 |
title_sort | salmonella enterica serovar paratyphi a-induced immune response in caenorhabditis elegans depends on mapk pathways and daf-16 |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10132459/ https://www.ncbi.nlm.nih.gov/pubmed/37122724 http://dx.doi.org/10.3389/fimmu.2023.1118003 |
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