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Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16

Salmonella enterica serovar Paratyphi A (S. Paratyphi A) is a pathogen that can cause enteric fever. According to the recent epidemic trends of typhoid fever, S. Paratyphi A has been the major important causative factor in paratyphoid fever. An effective vaccine for S. Paratyphi A has not been devel...

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Autores principales: Ding, Ai-Jun, Zhang, Wei-Ming, Tao, Jian, Chen, Bing, Liu, Xiao-Cao, Dong, Yu, Ma, Han-Jing, Pan, Shao-Dong, He, Jiang-Bo, Zeng, Wei-Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10132459/
https://www.ncbi.nlm.nih.gov/pubmed/37122724
http://dx.doi.org/10.3389/fimmu.2023.1118003
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author Ding, Ai-Jun
Zhang, Wei-Ming
Tao, Jian
Chen, Bing
Liu, Xiao-Cao
Dong, Yu
Ma, Han-Jing
Pan, Shao-Dong
He, Jiang-Bo
Zeng, Wei-Kun
author_facet Ding, Ai-Jun
Zhang, Wei-Ming
Tao, Jian
Chen, Bing
Liu, Xiao-Cao
Dong, Yu
Ma, Han-Jing
Pan, Shao-Dong
He, Jiang-Bo
Zeng, Wei-Kun
author_sort Ding, Ai-Jun
collection PubMed
description Salmonella enterica serovar Paratyphi A (S. Paratyphi A) is a pathogen that can cause enteric fever. According to the recent epidemic trends of typhoid fever, S. Paratyphi A has been the major important causative factor in paratyphoid fever. An effective vaccine for S. Paratyphi A has not been developed, which made it a tricky public health concern. Until now, how S. Paratyphi A interacts with organisms remain unknown. Here using lifespan assay, we found that S. Paratyphi A could infect Caenorhabditis elegans (C. elegans) at 25°C, and attenuate thermotolerance. The immune response of C. elegans was mediated by tir-1, nsy-1, sek-1, pmk-1, mpk-1, skn-1, daf-2 and daf-16, suggesting that S. Paratyphi A could regulate the MAPK and insulin pathways. Furthermore, we observed several phenotypical changes when C. elegans were fed S. Paratyphi A, including an accelerated decline in body movement, reduced the reproductive capacity, shortened spawning cycle, strong preference for OP50, arrested pharyngeal pumping and colonization of the intestinal lumen. The virulence of S. Paratyphi A requires living bacteria and is not mediated by secreting toxin. Using hydrogen peroxide analysis and quantitative RT-PCR, we discovered that S. Paratyphi A could increase oxidative stress and regulate the immune response in C. elegans. Our results sheds light on the infection mechanisms of S. Paratyphi A and lays a foundation for drugs and vaccine development.
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spelling pubmed-101324592023-04-27 Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16 Ding, Ai-Jun Zhang, Wei-Ming Tao, Jian Chen, Bing Liu, Xiao-Cao Dong, Yu Ma, Han-Jing Pan, Shao-Dong He, Jiang-Bo Zeng, Wei-Kun Front Immunol Immunology Salmonella enterica serovar Paratyphi A (S. Paratyphi A) is a pathogen that can cause enteric fever. According to the recent epidemic trends of typhoid fever, S. Paratyphi A has been the major important causative factor in paratyphoid fever. An effective vaccine for S. Paratyphi A has not been developed, which made it a tricky public health concern. Until now, how S. Paratyphi A interacts with organisms remain unknown. Here using lifespan assay, we found that S. Paratyphi A could infect Caenorhabditis elegans (C. elegans) at 25°C, and attenuate thermotolerance. The immune response of C. elegans was mediated by tir-1, nsy-1, sek-1, pmk-1, mpk-1, skn-1, daf-2 and daf-16, suggesting that S. Paratyphi A could regulate the MAPK and insulin pathways. Furthermore, we observed several phenotypical changes when C. elegans were fed S. Paratyphi A, including an accelerated decline in body movement, reduced the reproductive capacity, shortened spawning cycle, strong preference for OP50, arrested pharyngeal pumping and colonization of the intestinal lumen. The virulence of S. Paratyphi A requires living bacteria and is not mediated by secreting toxin. Using hydrogen peroxide analysis and quantitative RT-PCR, we discovered that S. Paratyphi A could increase oxidative stress and regulate the immune response in C. elegans. Our results sheds light on the infection mechanisms of S. Paratyphi A and lays a foundation for drugs and vaccine development. Frontiers Media S.A. 2023-04-12 /pmc/articles/PMC10132459/ /pubmed/37122724 http://dx.doi.org/10.3389/fimmu.2023.1118003 Text en Copyright © 2023 Ding, Zhang, Tao, Chen, Liu, Dong, Ma, Pan, He and Zeng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Ding, Ai-Jun
Zhang, Wei-Ming
Tao, Jian
Chen, Bing
Liu, Xiao-Cao
Dong, Yu
Ma, Han-Jing
Pan, Shao-Dong
He, Jiang-Bo
Zeng, Wei-Kun
Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16
title Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16
title_full Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16
title_fullStr Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16
title_full_unstemmed Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16
title_short Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16
title_sort salmonella enterica serovar paratyphi a-induced immune response in caenorhabditis elegans depends on mapk pathways and daf-16
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10132459/
https://www.ncbi.nlm.nih.gov/pubmed/37122724
http://dx.doi.org/10.3389/fimmu.2023.1118003
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