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Oxylipin biosynthetic gene families of Cannabis sativa

Cannabis sativa is a global multi-billion-dollar cash crop with numerous industrial uses, including in medicine and recreation where its value is largely owed to the production of pharmacological and psychoactive metabolites known as cannabinoids. Often underappreciated in this role, the lipoxygenas...

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Autores principales: Borrego, Eli J., Robertson, Mariah, Taylor, James, Schultzhaus, Zachary, Espinoza, Elida M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10132601/
https://www.ncbi.nlm.nih.gov/pubmed/37099560
http://dx.doi.org/10.1371/journal.pone.0272893
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author Borrego, Eli J.
Robertson, Mariah
Taylor, James
Schultzhaus, Zachary
Espinoza, Elida M.
author_facet Borrego, Eli J.
Robertson, Mariah
Taylor, James
Schultzhaus, Zachary
Espinoza, Elida M.
author_sort Borrego, Eli J.
collection PubMed
description Cannabis sativa is a global multi-billion-dollar cash crop with numerous industrial uses, including in medicine and recreation where its value is largely owed to the production of pharmacological and psychoactive metabolites known as cannabinoids. Often underappreciated in this role, the lipoxygenase (LOX)-derived green leaf volatiles (GLVs), also known as the scent of cut grass, are the hypothetical origin of hexanoic acid, the initial substrate for cannabinoid biosynthesis. The LOX pathway is best known as the primary source of plant oxylipins, molecules analogous to the eicosanoids from mammalian systems. These molecules are a group of chemically and functionally diverse fatty acid-derived signals that govern nearly all biological processes including plant defense and development. The interaction between oxylipin and cannabinoid biosynthetic pathways remains to be explored. Despite their unique importance in this crop, there has not been a comprehensive investigation focusing on the genes responsible for oxylipin biosynthesis in any Cannabis species. This study documents the first genome-wide catalogue of the Cannabis sativa oxylipin biosynthetic genes and identified 21 LOX, five allene oxide synthases (AOS), three allene oxide cyclases (AOC), one hydroperoxide lyase (HPL), and five 12-oxo-phytodienoic acid reductases (OPR). Gene collinearity analysis found chromosomal regions containing several isoforms maintained across Cannabis, Arabidopsis, and tomato. Promoter, expression, weighted co-expression genetic network, and functional enrichment analysis provide evidence of tissue- and cultivar-specific transcription and roles for distinct isoforms in oxylipin and cannabinoid biosynthesis. This knowledge facilitates future targeted approaches towards Cannabis crop improvement and for the manipulation of cannabinoid metabolism.
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spelling pubmed-101326012023-04-27 Oxylipin biosynthetic gene families of Cannabis sativa Borrego, Eli J. Robertson, Mariah Taylor, James Schultzhaus, Zachary Espinoza, Elida M. PLoS One Research Article Cannabis sativa is a global multi-billion-dollar cash crop with numerous industrial uses, including in medicine and recreation where its value is largely owed to the production of pharmacological and psychoactive metabolites known as cannabinoids. Often underappreciated in this role, the lipoxygenase (LOX)-derived green leaf volatiles (GLVs), also known as the scent of cut grass, are the hypothetical origin of hexanoic acid, the initial substrate for cannabinoid biosynthesis. The LOX pathway is best known as the primary source of plant oxylipins, molecules analogous to the eicosanoids from mammalian systems. These molecules are a group of chemically and functionally diverse fatty acid-derived signals that govern nearly all biological processes including plant defense and development. The interaction between oxylipin and cannabinoid biosynthetic pathways remains to be explored. Despite their unique importance in this crop, there has not been a comprehensive investigation focusing on the genes responsible for oxylipin biosynthesis in any Cannabis species. This study documents the first genome-wide catalogue of the Cannabis sativa oxylipin biosynthetic genes and identified 21 LOX, five allene oxide synthases (AOS), three allene oxide cyclases (AOC), one hydroperoxide lyase (HPL), and five 12-oxo-phytodienoic acid reductases (OPR). Gene collinearity analysis found chromosomal regions containing several isoforms maintained across Cannabis, Arabidopsis, and tomato. Promoter, expression, weighted co-expression genetic network, and functional enrichment analysis provide evidence of tissue- and cultivar-specific transcription and roles for distinct isoforms in oxylipin and cannabinoid biosynthesis. This knowledge facilitates future targeted approaches towards Cannabis crop improvement and for the manipulation of cannabinoid metabolism. Public Library of Science 2023-04-26 /pmc/articles/PMC10132601/ /pubmed/37099560 http://dx.doi.org/10.1371/journal.pone.0272893 Text en © 2023 Borrego et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Borrego, Eli J.
Robertson, Mariah
Taylor, James
Schultzhaus, Zachary
Espinoza, Elida M.
Oxylipin biosynthetic gene families of Cannabis sativa
title Oxylipin biosynthetic gene families of Cannabis sativa
title_full Oxylipin biosynthetic gene families of Cannabis sativa
title_fullStr Oxylipin biosynthetic gene families of Cannabis sativa
title_full_unstemmed Oxylipin biosynthetic gene families of Cannabis sativa
title_short Oxylipin biosynthetic gene families of Cannabis sativa
title_sort oxylipin biosynthetic gene families of cannabis sativa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10132601/
https://www.ncbi.nlm.nih.gov/pubmed/37099560
http://dx.doi.org/10.1371/journal.pone.0272893
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