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A decline in avian cytokine expression with age revealed by commercially available cytokine array
Cytokines are secreted immunomodulators that are key regulators of the avian immune response. Currently, the most commonly used method to follow cytokine expression is qPCR, which measures cellular levels of mRNA, rather their extracellular circulating levels. Here we present a commercially availabl...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10132730/ https://www.ncbi.nlm.nih.gov/pubmed/37123272 http://dx.doi.org/10.3389/fphys.2023.1171782 |
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author | Levkovich, Guy Almagor, Dana Saibaba, Ganesan Bendikov-Bar, Inna Rusal, Mark Lokshtanov, Dmitri Shinder, Dmitri Sagi, Dror |
author_facet | Levkovich, Guy Almagor, Dana Saibaba, Ganesan Bendikov-Bar, Inna Rusal, Mark Lokshtanov, Dmitri Shinder, Dmitri Sagi, Dror |
author_sort | Levkovich, Guy |
collection | PubMed |
description | Cytokines are secreted immunomodulators that are key regulators of the avian immune response. Currently, the most commonly used method to follow cytokine expression is qPCR, which measures cellular levels of mRNA, rather their extracellular circulating levels. Here we present a commercially available cytokine array designed to assay circulating expression levels of multiple cytokines and immunomodulators simultaneously. Upon minor modifications to the manufacturer protocol, background noise was reduced, leading to a significant increase in the sensitivity of the device. Our data indicate that the array is reliable and produce consistent data between biological repeats. We tested the reproducibility of the array in a biologically relevant context by assessing age-related changes in circulating cytokines. While individual features did not show a consistent pattern, our data revealed a consistent decline in the median of all cytokine values, supporting the validity of the array in studying biological processes. |
format | Online Article Text |
id | pubmed-10132730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101327302023-04-27 A decline in avian cytokine expression with age revealed by commercially available cytokine array Levkovich, Guy Almagor, Dana Saibaba, Ganesan Bendikov-Bar, Inna Rusal, Mark Lokshtanov, Dmitri Shinder, Dmitri Sagi, Dror Front Physiol Physiology Cytokines are secreted immunomodulators that are key regulators of the avian immune response. Currently, the most commonly used method to follow cytokine expression is qPCR, which measures cellular levels of mRNA, rather their extracellular circulating levels. Here we present a commercially available cytokine array designed to assay circulating expression levels of multiple cytokines and immunomodulators simultaneously. Upon minor modifications to the manufacturer protocol, background noise was reduced, leading to a significant increase in the sensitivity of the device. Our data indicate that the array is reliable and produce consistent data between biological repeats. We tested the reproducibility of the array in a biologically relevant context by assessing age-related changes in circulating cytokines. While individual features did not show a consistent pattern, our data revealed a consistent decline in the median of all cytokine values, supporting the validity of the array in studying biological processes. Frontiers Media S.A. 2023-04-12 /pmc/articles/PMC10132730/ /pubmed/37123272 http://dx.doi.org/10.3389/fphys.2023.1171782 Text en Copyright © 2023 Levkovich, Almagor, Saibaba, Bendikov-Bar, Rusal, Lokshtanov, Shinder and Sagi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Levkovich, Guy Almagor, Dana Saibaba, Ganesan Bendikov-Bar, Inna Rusal, Mark Lokshtanov, Dmitri Shinder, Dmitri Sagi, Dror A decline in avian cytokine expression with age revealed by commercially available cytokine array |
title | A decline in avian cytokine expression with age revealed by commercially available cytokine array |
title_full | A decline in avian cytokine expression with age revealed by commercially available cytokine array |
title_fullStr | A decline in avian cytokine expression with age revealed by commercially available cytokine array |
title_full_unstemmed | A decline in avian cytokine expression with age revealed by commercially available cytokine array |
title_short | A decline in avian cytokine expression with age revealed by commercially available cytokine array |
title_sort | decline in avian cytokine expression with age revealed by commercially available cytokine array |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10132730/ https://www.ncbi.nlm.nih.gov/pubmed/37123272 http://dx.doi.org/10.3389/fphys.2023.1171782 |
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