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A decline in avian cytokine expression with age revealed by commercially available cytokine array

Cytokines are secreted immunomodulators that are key regulators of the avian immune response. Currently, the most commonly used method to follow cytokine expression is qPCR, which measures cellular levels of mRNA, rather their extracellular circulating levels. Here we present a commercially availabl...

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Autores principales: Levkovich, Guy, Almagor, Dana, Saibaba, Ganesan, Bendikov-Bar, Inna, Rusal, Mark, Lokshtanov, Dmitri, Shinder, Dmitri, Sagi, Dror
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10132730/
https://www.ncbi.nlm.nih.gov/pubmed/37123272
http://dx.doi.org/10.3389/fphys.2023.1171782
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author Levkovich, Guy
Almagor, Dana
Saibaba, Ganesan
Bendikov-Bar, Inna
Rusal, Mark
Lokshtanov, Dmitri
Shinder, Dmitri
Sagi, Dror
author_facet Levkovich, Guy
Almagor, Dana
Saibaba, Ganesan
Bendikov-Bar, Inna
Rusal, Mark
Lokshtanov, Dmitri
Shinder, Dmitri
Sagi, Dror
author_sort Levkovich, Guy
collection PubMed
description Cytokines are secreted immunomodulators that are key regulators of the avian immune response. Currently, the most commonly used method to follow cytokine expression is qPCR, which measures cellular levels of mRNA, rather their extracellular circulating levels. Here we present a commercially available cytokine array designed to assay circulating expression levels of multiple cytokines and immunomodulators simultaneously. Upon minor modifications to the manufacturer protocol, background noise was reduced, leading to a significant increase in the sensitivity of the device. Our data indicate that the array is reliable and produce consistent data between biological repeats. We tested the reproducibility of the array in a biologically relevant context by assessing age-related changes in circulating cytokines. While individual features did not show a consistent pattern, our data revealed a consistent decline in the median of all cytokine values, supporting the validity of the array in studying biological processes.
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spelling pubmed-101327302023-04-27 A decline in avian cytokine expression with age revealed by commercially available cytokine array Levkovich, Guy Almagor, Dana Saibaba, Ganesan Bendikov-Bar, Inna Rusal, Mark Lokshtanov, Dmitri Shinder, Dmitri Sagi, Dror Front Physiol Physiology Cytokines are secreted immunomodulators that are key regulators of the avian immune response. Currently, the most commonly used method to follow cytokine expression is qPCR, which measures cellular levels of mRNA, rather their extracellular circulating levels. Here we present a commercially available cytokine array designed to assay circulating expression levels of multiple cytokines and immunomodulators simultaneously. Upon minor modifications to the manufacturer protocol, background noise was reduced, leading to a significant increase in the sensitivity of the device. Our data indicate that the array is reliable and produce consistent data between biological repeats. We tested the reproducibility of the array in a biologically relevant context by assessing age-related changes in circulating cytokines. While individual features did not show a consistent pattern, our data revealed a consistent decline in the median of all cytokine values, supporting the validity of the array in studying biological processes. Frontiers Media S.A. 2023-04-12 /pmc/articles/PMC10132730/ /pubmed/37123272 http://dx.doi.org/10.3389/fphys.2023.1171782 Text en Copyright © 2023 Levkovich, Almagor, Saibaba, Bendikov-Bar, Rusal, Lokshtanov, Shinder and Sagi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Levkovich, Guy
Almagor, Dana
Saibaba, Ganesan
Bendikov-Bar, Inna
Rusal, Mark
Lokshtanov, Dmitri
Shinder, Dmitri
Sagi, Dror
A decline in avian cytokine expression with age revealed by commercially available cytokine array
title A decline in avian cytokine expression with age revealed by commercially available cytokine array
title_full A decline in avian cytokine expression with age revealed by commercially available cytokine array
title_fullStr A decline in avian cytokine expression with age revealed by commercially available cytokine array
title_full_unstemmed A decline in avian cytokine expression with age revealed by commercially available cytokine array
title_short A decline in avian cytokine expression with age revealed by commercially available cytokine array
title_sort decline in avian cytokine expression with age revealed by commercially available cytokine array
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10132730/
https://www.ncbi.nlm.nih.gov/pubmed/37123272
http://dx.doi.org/10.3389/fphys.2023.1171782
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